An enzyme-linked immunosorbent assay for cow's milk protein-specific IgE using biotinylated antigen

1986 ◽  
Vol 90 (2) ◽  
pp. 241-246 ◽  
Author(s):  
Alessandro Plebani ◽  
Alberto G. Ugazio ◽  
Antonietta M. Avanzini ◽  
Virginia Monafo ◽  
G. Roberto Burgio
Keyword(s):  
Immunosorbent Assay ◽  
Milk Protein ◽  
Specific Ige ◽  
Cow’S Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cow's Milk ◽  
Cow’S Milk Protein

scholarly journals Immunological biomarkers changes during diet therapy in children with food allergy

2015 ◽  
Vol 12 (1) ◽  
pp. 73-78
Author(s):  
I V Vorozhko ◽  
T B Sentsova ◽  
V A Revyakina ◽  
O O Chernyak ◽  
S N Denisova
Keyword(s):  
Food Allergy ◽  
Milk Protein ◽  
Goat Milk ◽  
Specific Ige ◽  
Diet Therapy ◽  
Cow’S Milk ◽  
Igg Antibodies ◽  
Cow's Milk ◽  
Cow’S Milk Protein ◽  
Different Sources

Immunological estimating of different diet therapy types became currently very important. The aim of this work was to study the changes of immunological biomarkers during usage of formulas based on different sources of protein in children with food allergy and to assess and predict the effectiveness of diet therapy. The study included 66 children with food allergy in age from 1,5 months to 1,5 years. Observed children were divided into three groups depending on the type of formulas used. The dynamics of immunoregulatory substances (IL-5, TGF β 1, IL-10, IL-4, IL-2, IL-13, Eotaxin3), and allergenspecific IgE and IgG antibodies to cow’s milk protein, α-lactoglobulin, β-laktoglobulin, casein, soy and goat’s milk during diet therapy were evaluated. Observation duration was 28-30 days. The differences in the dynamics of the investigated parameters depending on the type of formula were revealed. Thus, the use of formula based on cow’s milk hydrolysates revealed their primarily effect on cytokines IL-2, IL-5 and growth factor TGF β ; the use of formula based on soy protein revealed combined effect on humoral immunity (allergen specific IgE and IgG antibodies) and IL-5; the use of formula based on goat milk characterized decreased levels of allergenspecific IgG antibodies to the cow’s milk protein and its fractions, reduced allergen specific IgE antibody concentrations to cow’s milk protein and α-lactoglobulin, decreased levels of IL-5, and, exclusive for this formula - reduction of IL-4 level. Accordingly, the assessment of immunological biomarkers can be used to personalize diet therapy in children with food allergy.

scholarly journals Algorithm of conducting oral provocation tests with milk in 1–3-year-old children: analysis of existing standards and own experience

2021 ◽  
Keyword(s):  
Milk Protein ◽  
Specific Ige ◽  
Physical Development ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Oral Provocation ◽  
Provocation Tests ◽  
Cow’S Milk Protein

Introduction. Food allergy is an urgent problem in modern pediatrics. Cow’s milk is the main food allergen in young children. By the age of one year, from 0.5 % to 3 % of children suffer from cow’s milk protein allergy. Aim. To analyze the existing standards of conducting oral provocation tests with milk in young children and suggest own algorithm of diagnostics. Materials and methods. Algorithm of diagnosing cow’s milk allergy was probated in 107 children aged from one to three years in Communal Municipal Children’s Clinical Hospital. To diagnose cow’s milk allergy, compilation of allergological anamnesis, examination, assessment of physical development, and determination of specific IgE to cow’s milk are performed. Then, different oral provocation tests are conducted based on the obtained results: at the allergist’s office on an outpatient basis, in a specialized inpatient department, in an intensive care unit. Results. Among 107 children, who were subjected to oral provocation test with milk, cow’s milk protein allergy was diagnosed in 67 (62.62 %). In children, who were diagnosed cow’s milk protein allergy (n = 67), skin (41.79 %) and gastrointestinal (28.36 %) symptoms prevailed. In the group of children with predictable low risk of allergy, diameter of a papule equaled 3.86 ± 0.62 mm2, the level of specific IgE to milk in blood serum was 0.58 [0.37–0.68], and in the group with medium risk – 5.51 ± 1.38 mm2 and 3.62 [0.86–9.66] kU/L, respectively. All 40 children, in whom cow’s milk protein allergy was not confirmed, constituted the group with predictable low risk of reaction development. Diameter of a papule during prick-test equaled 3.13 ± 0.24 mm2, the level of specific IgE to milk in blood serum was 0.36 [0.35–0.67] kU/L. In this group of children, complaints of gastrointestinal symptoms (82.5 %) prevailed. Conclusions. A complex diagnostic approach, in particular, study of anamnesis, examination, assessment of physical development, record of food diary, determination of specific IgE to milk, oral provocation tests enable to optimize diagnostics of food allergy and choose further therapeutic tactics. Algorithm of diagnosing cow’s milk protein allergy in 1–3-year-old children has been elaborated, which can be recommended for common application in clinical practice.

scholarly journals DIAGNOSTIC PREDICTORS OF TOLERANCE DEVELOPMENT IN THE ELIMINATION DIET IN CHILDREN WITH COW'S MILK PROTEIN ALLERGY

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Oksana Matsyura ◽  
◽  
Lesya Besh ◽  
Natalia Lukyanenko ◽  
Oksana Kovalska ◽  
...  
Keyword(s):  
Milk Protein ◽  
Tolerance Induction ◽  
Milk Proteins ◽  
Specific Ige ◽  
Elimination Diet ◽  
Cow’S Milk ◽  
Molecular Profile ◽  
Tolerance Development ◽  
Cow's Milk ◽  
Cow’S Milk Protein

Background. Cow's milk protein allergy is a topical issue in pediatrics. This disease affects 2-3% of young children. Methods. The study included 30 children with a confirmed cow’s milk protein allergy and a prescribed milk elimination diet. The children were observed for 36 months. The molecular profile was evaluated by detecting the major components of milk (Bos d 8, Bos d 5, Bos d 4), minor (Bos d 6), and cross-reactive with serum albumin (Fel d 2, Can f 3). Results. The study group included 46.7% of boys. The mean age of the subjects was 14.5±3.18 months. Nine of the 25 analyzed factors were identified in the course of our work by the method of logistic regression with progressive selection, which in combined action affect the formation of tolerance to cow's milk proteins in allergic children. There are three clinical examples of calculating the prediction of the complete tolerance development in the elimination diet in children with cow's milk protein allergy. Conclusions. Independent predictors that reduce the likelihood of the tolerance development in the elimination diet, have identified: skin test to milk, Bos d 8, Bos d 5, Bos d 4, Bos d 6. Independent predictors of the tolerance development in the elimination diet are: milk-specific IgE, total IgE, Fel d 2, Can f 3. Low probability of tolerance development by elimination therapy is a recommendation for prescribing treatment to a patient by specific oral tolerance induction.

scholarly journals Assessment of IgE-Mediated and Non-IgE-Mediated Cow’s Milk Protein Allergy in Children

2019 ◽  
Vol 25 (3) ◽  
pp. 129-131
Author(s):  
Balasa Adriana ◽  
Mihai Cristina ◽  
Pruna Irina ◽  
Chisnoiu Tatiana ◽  
Daineanu Diana ◽  
...  
Keyword(s):  
Primary Care Physicians ◽  
Milk Protein ◽  
Immunoglobulin E ◽  
Clinical Manifestations ◽  
Specific Ige ◽  
Cow Milk ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Ige Mediated ◽  
Cow’S Milk Protein

Abstract Cow’s milk protein allergy (CMPA) is the most common food allergy found in children under 3 years of age. In most cases, it occurs in infancy. Early diagnosis and appropriate treatment can decrease the risk of impaired growth. In our study, we evaluated 40 children, with ages between 1 month and 3 years, diagnosed with IgE-mediated or non-IgE-mediated CMPA, from january to december 2017, in the Department of Pediatrics of the Clinical Emergency County Hospital of Constanta. The inclusion criteria consisted of: age, natural or artificial feeding, specific IgE levels, CoMiSS score, and clinical manifestations. The Cow’s Milk-related-Symptom-Score (CoMiSS) was developed as a screening and diagnostic tool for CMPA prediction, and can guide pediatricians and primary care physicians to make an early diagnostic, as it can be easily missed. We observed a higher number of cases of CMPA registered among children who were artificially fed (57,5%), followed by those with mixed nutrition (25%), the remaining (17,5%) being represented by exclusively breastfed infants. The most frequent clinical manifestations were rashes (87,5%), failure to thrive (82,5%), regurgitation (50%) and diarrhea (35%). Further data should be collected to prove if the association between a CoMiSS score higher than 12 and specific IgE-mediated CMPA is clinically relevant, and can predict, based on clinical examination and anamnesis, high serum levels of specific immunoglobulin E. The prognosis can depend on the titre of specific IgE at the time of diagnosis, as they are more likely to develop several crossed allergies and less prone to become tolerant to cow milk proteins than those with non-IgE-mediated CMPA.

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