Detection by Competitive Enzyme-Linked Immunosorbent Assay of a Bovine Serum Albumin Peptide (ABBOS) in Infant Formulas Based on Hydrolyzed Cow's Milk Protein

Diabetes Care ◽  
1996 ◽  
Vol 19 (12) ◽  
pp. 1364-1369 ◽  
Author(s):  
E. C. H. Van Beresteijn ◽  
R. J. G. M. Meijer
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Immunosorbent Assay ◽  
Bovine Serum ◽  
Milk Protein ◽  
Cow’S Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infant Formulas ◽  
Cow's Milk ◽  
Cow’S Milk Protein

An enzyme-linked immunosorbent assay for cow's milk protein-specific IgE using biotinylated antigen

1986 ◽  
Vol 90 (2) ◽  
pp. 241-246 ◽  
Author(s):  
Alessandro Plebani ◽  
Alberto G. Ugazio ◽  
Antonietta M. Avanzini ◽  
Virginia Monafo ◽  
G. Roberto Burgio
Keyword(s):  
Immunosorbent Assay ◽  
Milk Protein ◽  
Specific Ige ◽  
Cow’S Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cow's Milk ◽  
Cow’S Milk Protein

scholarly journals Easy Enzyme-Linked Immunosorbent Assay for Spectinomycin in Chicken Plasma

1996 ◽  
Vol 79 (2) ◽  
pp. 426-430 ◽  
Author(s):  
Touichi Tanaka ◽  
Hideharu Ikebuchi ◽  
Jun-Ichi Sawada ◽  
Mariko Okada ◽  
Yasumasa Kido
Keyword(s):  
Detection Limit ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Immunosorbent Assay ◽  
Bovine Serum ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Carrier Protein ◽  
Bovine Serum Albumin Conjugate

Abstract An easy, sensitive, competitive indirect enzyme- linked immunosorbent assay (CI-ELISA) for specti nomycin in chicken plasma was developed. Preparation of a spectinomycin-bovine serum albumin conjugate in which the hapten is linked to the carrier protein through the C-4 position is described. Antibodies raised against antigens in rabbits had excellent specificity for spectinomycin, exhibiting a cross-reactivity of 44.0% with dihydrospectinomy-cin and 13.8% with tetrahydrospectinomycin. No cross-reactivity was observed with other antibiotics. The detection limit of the CI-ELISA was 2 ng/mL (equivalent into 40 ng/mL undiluted chicken plasma) spectinomycin. Known amounts (0.1-100 μg/mL) of spectinomycin were added to chicken plasma and then analyzed. Average recoveries were 97-110%. This procedure may be used without prior extraction of samples.

scholarly journals KONJUGASI ANHIDRAT KlORAMFENIKOL-PROTEIN (CAP-BSA DAN CAP-KLH) UNTUK PRODUKSI IgG DARI SERUM KELINCI

2011 ◽  
Vol 1 (2) ◽  
pp. 23-31
Author(s):  
Musyirna Rahmah Nst ◽  
Tri Budhi Murdiati
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Immunosorbent Assay ◽  
Bovine Serum ◽  
Keyhole Limpet Hemocyanin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Bovine Serum Albumin

 Dalam pengembangan metoda analisis residu antibiotik pada produk pangan hewani secara immunokimia telah dilakukan sintesis imunogen kloramfenikol (CAP) dengan protein Bovine Serum Albumin (BSA) dan Keyhole Limpet Hemocyanin (KLH) dengan metoda konjugasi mixed anhidrad. Hasil sintesis digunakan untuk produksi antibodi.Antigen dan antibodi yang dihasilkan dijadikan prangkat analisis residu antibiotik dengan Competitive Enzyme Linked Immunosorbent Assay (ELISA kompetitif). Hasil penelitian menunjukkan bahwa metoda konjugasi mixed anhidrad pada preparasi imunogen dengan jumlah CAP yang terkonjugasi ke grup amino bebas dari molekul protein BSA (CAP-BSA) adalah 18 unit dan ke protein KLH (CAP-KLH) adalah 782 unit. Antibodi poliklonal CAP-BSA dan CAP-KLH yang diproduksi dengan rata-rata kandungan IgG CAP-BSA 2,07 mg/ml dan IgG CAP-KLH 2,21 mg/ml.

scholarly journals Milk and cow’s meat allergy in a child: A clinical case

2021 ◽  
Vol 29 (4) ◽  
Author(s):  
Maria Marques ◽  
◽  
Inês Falcão ◽  
Moisés Labrador-Horrillo ◽  
Helena Falcão ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Milk Proteins ◽  
Food Challenge ◽  
Specific Ige ◽  
Oral Food Challenge ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Cow’S Milk Proteins

Allergy to bovine serum albumin is the main predictor of beef allergy associated with cow’s milk proteins allergy. We report a case of a 3-year-old child with cow’s milk proteins allergy since the age of 6 months who, after some ingestions of beef, developed episodes of irritability, urticaria and syncope. Specific IgE to beef, oral food challenge with medium rare cooked beef and specific IgE to bovine serum albumin were all positive, but an oral food challenge with well cooked beef was tolerated. Allergy to bovine serum albumin is not usually associated with severe reactions, since it is a thermolabile protein, however, the process of cooking meat may be insufficient to have an effect on the complex matrix of meat and associated serum albumins. The irregular pattern of the episodes and the previous diagnosis of cow’s milk proteins allergy may act as confounding factors leading to a delayed diagnosis.

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