The use of monoclonal antibodies specific for seal immunoglobulins in an enzyme-linked immunosorbent assay to detect canine distemper virus-specific immunoglobulin in seal plasma samples

1993 ◽  
Vol 160 (2) ◽  
pp. 163-171 ◽  
Author(s):  
D.P. King ◽  
A.W.M. Hay ◽  
I. Robinson ◽  
S.W. Evans
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Canine Distemper Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Canine Distemper ◽  
Specific Immunoglobulin

scholarly journals Development of a double monoclonal antibody–based sandwich enzyme-linked immunosorbent assay for detecting canine distemper virus

2020 ◽  
Vol 104 (24) ◽  
pp. 10725-10735
Author(s):  
Yuan Zhang ◽  
Gang Xu ◽  
Lu Zhang ◽  
Jiakai Zhao ◽  
Pinpin Ji ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Canine Distemper Virus ◽  
Sandwich Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
Canine Distemper ◽  
Serum Samples ◽  
Ideal Method

Abstract Canine distemper virus (CDV) infection causes mass mortality in diverse carnivore species. For effective virus surveillance, rapid and sensitive assays are needed to detect CDV in field samples. In this study, after BABL/c mice were immunized with recombinant CDV-fusion (F) protein, monoclonal antibodies (mAbs) against recombinant CDV-F protein (designated 1A5, 1A6, and 7D5) were produced using traditional hybridoma cell technology. Next, capture antibody (1A6, 800 ng/well) and horseradish peroxidase (HRP)–conjugated detection antibody (HRP-7D5, 1:100, 500 ng/well) were used in a double monoclonal antibody–based sandwich enzyme-linked immunosorbent assay (ELISA) for CDV detection after optimization of both mAb amounts per well using a checkerboard titration test. Based on sandwich ELISA test results for 120 known CDV-negative samples, the cutoff value for a positive result was set to an OD450 nm value ≥ 0.196. As compared with test results obtained from commercial immune colloidal gold test strips, the low limits of detection for the two assays were revealed to be 100 TCID50 per 100 μL. In addition, the sandwich ELISA agreed 100% and 96.4% with commercial immune colloidal gold test strips when testing serum and stool samples. The sandwich ELISA assay provided statistically similar CDV detection. Thus, the sandwich ELISA developed here to detect CDV in fecal and serum samples provided good sensitivity, high specificity, and good reproducibility and should serve as an ideal method for large-scale surveillance of CDV infections in carnivores. Key points • Three CDV mAbs that recognized different epitopes and bound to virion were generated. • The sandwich ELISA based mAbs to detect CDV in fecal and serum samples was developed. • The sandwich ELISA is an ideal method for detecting CDV infections in the field.

scholarly journals Serological Survey of Canine Distemper Virus Infection Using Enzyme-Linked Immunosorbent Assay.

1995 ◽  
Vol 57 (4) ◽  
pp. 761-763 ◽  
Author(s):  
Tsuyoshi GEMMA ◽  
Naoko MIYASHITA ◽  
Yeon-Sil SHIN ◽  
Masatsugu OKITA ◽  
Takeshi MORI ◽  
...  
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Canine Distemper Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Survey ◽  
Canine Distemper

scholarly journals Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibodies for Direct Serotyping of Enteric Adenoviruses in Feces

1991 ◽  
Vol 65 (5) ◽  
pp. 552-558 ◽  
Author(s):  
Kenji TAKAGI ◽  
Yasutaka YAMASHITA ◽  
Hiroo INOUYE ◽  
Mitsuaki OHSETO ◽  
Hiroko KUWABARA ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enteric Adenoviruses

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Rapid detection of human rotavirus strains in stools by single-sandwich enzyme-linked immunosorbent assay systems using monoclonal antibodies

1989 ◽  
Vol 24 (1-2) ◽  
pp. 43-56 ◽  
Author(s):  
Giuseppe Gerna ◽  
Antonella Sarasini ◽  
Angela Di Matteo ◽  
Maurizio Parea ◽  
Maria Torseilini ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Rotavirus
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