scholarly journals Use of hydrophilic extra-viral domain of canine distemper virus H protein for enzyme-linked immunosorbent assay development

2014 ◽  
Vol 15 (4) ◽  
pp. 503 ◽  
Author(s):  
Ki-hyun Cho ◽  
Jeongmi Kim ◽  
Hyun-ah Yoo ◽  
Dae-hee Kim ◽  
Seung-yong Park ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Canine Distemper Virus ◽  
Assay Development ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Distemper ◽  
H Protein

scholarly journals Development of a double monoclonal antibody–based sandwich enzyme-linked immunosorbent assay for detecting canine distemper virus

2020 ◽  
Vol 104 (24) ◽  
pp. 10725-10735
Author(s):  
Yuan Zhang ◽  
Gang Xu ◽  
Lu Zhang ◽  
Jiakai Zhao ◽  
Pinpin Ji ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Canine Distemper Virus ◽  
Sandwich Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
Canine Distemper ◽  
Serum Samples ◽  
Ideal Method

Abstract Canine distemper virus (CDV) infection causes mass mortality in diverse carnivore species. For effective virus surveillance, rapid and sensitive assays are needed to detect CDV in field samples. In this study, after BABL/c mice were immunized with recombinant CDV-fusion (F) protein, monoclonal antibodies (mAbs) against recombinant CDV-F protein (designated 1A5, 1A6, and 7D5) were produced using traditional hybridoma cell technology. Next, capture antibody (1A6, 800 ng/well) and horseradish peroxidase (HRP)–conjugated detection antibody (HRP-7D5, 1:100, 500 ng/well) were used in a double monoclonal antibody–based sandwich enzyme-linked immunosorbent assay (ELISA) for CDV detection after optimization of both mAb amounts per well using a checkerboard titration test. Based on sandwich ELISA test results for 120 known CDV-negative samples, the cutoff value for a positive result was set to an OD450 nm value ≥ 0.196. As compared with test results obtained from commercial immune colloidal gold test strips, the low limits of detection for the two assays were revealed to be 100 TCID50 per 100 μL. In addition, the sandwich ELISA agreed 100% and 96.4% with commercial immune colloidal gold test strips when testing serum and stool samples. The sandwich ELISA assay provided statistically similar CDV detection. Thus, the sandwich ELISA developed here to detect CDV in fecal and serum samples provided good sensitivity, high specificity, and good reproducibility and should serve as an ideal method for large-scale surveillance of CDV infections in carnivores. Key points • Three CDV mAbs that recognized different epitopes and bound to virion were generated. • The sandwich ELISA based mAbs to detect CDV in fecal and serum samples was developed. • The sandwich ELISA is an ideal method for detecting CDV infections in the field.

scholarly journals Serological Survey of Canine Distemper Virus Infection Using Enzyme-Linked Immunosorbent Assay.

1995 ◽  
Vol 57 (4) ◽  
pp. 761-763 ◽  
Author(s):  
Tsuyoshi GEMMA ◽  
Naoko MIYASHITA ◽  
Yeon-Sil SHIN ◽  
Masatsugu OKITA ◽  
Takeshi MORI ◽  
...  
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Canine Distemper Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Survey ◽  
Canine Distemper

scholarly journals Genetic diversity of the attachment (H) protein gene of current field isolates of canine distemper virus.

1997 ◽  
Vol 78 (2) ◽  
pp. 367-372 ◽  
Author(s):  
G Bolt ◽  
P Arctander ◽  
T D Jensen ◽  
M J Appel ◽  
E Gottschalck ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Canine Distemper Virus ◽  
Protein Gene ◽  
Canine Distemper ◽  
Current Field ◽  
Field Isolates ◽  
H Protein

scholarly journals Disease Duration Determines Canine Distemper Virus Neurovirulence

2007 ◽  
Vol 81 (21) ◽  
pp. 12066-12070 ◽  
Author(s):  
François Bonami ◽  
Penny A. Rudd ◽  
Veronika von Messling
Keyword(s):  
Canine Distemper Virus ◽  
Disease Duration ◽  
Subsequent Development ◽  
Neurological Involvement ◽  
Disease Course ◽  
Canine Distemper ◽  
Wild Type ◽  
Neurological Signs ◽  
H Protein ◽  
Type Strains

ABSTRACT The Morbillivirus hemagglutinin (H) protein mediates attachment to the target cell. To evaluate its contribution to canine distemper virus neurovirulence, we exchanged the H proteins of the wild-type strains 5804P and A75 and assessed the pathogenesis of the chimeric viruses in ferrets. Both strains are lethal to ferrets; however, 5804P causes a 2-week disease without neurological signs, whereas A75 is associated with a longer disease course and neurological involvement. We observed that both H proteins supported neuroinvasion and the subsequent development of clinical neurological signs if given enough time, demonstrating that disease duration is the main neurovirulence determinant.

scholarly journals Protective Immunity against Canine Distemper Virus in Dogs Induced by Intranasal Immunization with a Recombinant Probiotic Expressing the Viral H Protein

Vaccines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 213
Author(s):  
Yanping Jiang ◽  
Shuo Jia ◽  
Dianzhong Zheng ◽  
Fengsai Li ◽  
Shengwen Wang ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Immunoglobulin A ◽  
Canine Distemper ◽  
Intranasal Route ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Protection Efficacy ◽  
Specific Igg ◽  
High Level ◽  
H Protein

Canine distemper virus (CDV) elicits a severe contagious disease in a broad range of hosts. CDV mortality rates are 50% in domestic dogs and 100% in ferrets. Its primary infection sites are respiratory and intestinal mucosa. This study aimed to develop an effective mucosal CDV vaccine using a non-antibiotic marked probiotic pPGΔCm-T7g10-EGFP-H/L. casei 393 strain expressing the CDV H protein. Its immunogenicity in BALB/c mice was evaluated using intranasal and oral vaccinations, whereas in dogs the intranasal route was used for vaccination. Our results indicate that this probiotic vaccine can stimulate a high level of secretory immunoglobulin A (sIgA)-based mucosal and IgG-based humoral immune responses in mice. SIgA levels in the nasal lavage and lungs were significantly higher in intranasally vaccinated mice than those in orally vaccinated mice. Both antigen-specific IgG and sIgA antibodies were effectively elicited in dogs through the intranasal route and demonstrated superior immunogenicity. The immune protection efficacy of the probiotic vaccine was evaluated by challenging the immunized dogs with virulent CDV 42 days after primary immunization. Dogs of the pPGΔCm-T7g10-EGFP-H/L. casei 393 group were completely protected against CDV. The proposed probiotic vaccine could be promising for protection against CDV infection in dogs.

scholarly journals Serological detection of infection with canine distemper virus, canine parvovirus and canine adenovirus in communal dogs from Zimbabwe

2014 ◽  
Vol 85 (1) ◽  
Author(s):  
Anna McRee ◽  
Rebecca P. Wilkes ◽  
Jessica Dawson ◽  
Roger Parry ◽  
Chris Foggin ◽  
...  
Keyword(s):  
Disease Transmission ◽  
Canine Distemper Virus ◽  
Wildlife Conservation ◽  
Canine Parvovirus ◽  
Control Measures ◽  
Domestic Dogs ◽  
Sub Saharan Africa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Distemper ◽  
The Impact

Domestic dogs are common amongst communities in sub-Saharan Africa and may serve as important reservoirs for infectious agents that may cause diseases in wildlife. Two agents of concern are canine parvovirus (CPV) and canine distemper virus (CDV), which may infect and cause disease in large carnivore species such as African wild dogs and African lions, respectively. The impact of domestic dogs and their diseases on wildlife conservation is increasing in Zimbabwe, necessitating thorough assessment and implementation of control measures. In this study, domestic dogs in north-western Zimbabwe were evaluated for antibodies to CDV, CPV, and canine adenovirus (CAV). These dogs were communal and had no vaccination history. Two hundred and twenty-five blood samples were collected and tested using a commercial enzyme-linked immunosorbent assay (ELISA) for antibodies to CPV, CDV, and CAV. Of these dogs, 75 (34%) had detectable antibodies to CDV, whilst 191 (84%) had antibodies to CPV. Antibodies to canine adenovirus were present in 28 (13%) dogs. Canine parvovirus had high prevalence in all six geographic areas tested. These results indicate that CPV is circulating widely amongst domestic dogs in the region. In addition, CDV is present at high levels. Both pathogens can infect wildlife species. Efforts for conservation of large carnivores in Zimbabwe must address the role of domestic dogs in disease transmission.

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