Hormonal control of tanning in the American cockroach—II. Assay for the hormone and the effect of wound healing

1965 ◽  
Vol 11 (9) ◽  
pp. 1269-1275 ◽  
Author(s):  
Richard R. Mills
Keyword(s):  
Wound Healing ◽  
Hormonal Control ◽  
American Cockroach

scholarly journals Cloning, Expression and Effects of P. americana Thymosin on Wound Healing

2019 ◽  
Vol 20 (19) ◽  
pp. 4932 ◽  
Author(s):  
Jie Jing ◽  
Xiaohong Sun ◽  
Chuang Zhou ◽  
Yifan Zhang ◽  
Yongmei Shen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Tissue Regeneration ◽  
Dna Sequences ◽  
Wound Repair ◽  
Periplaneta Americana ◽  
American Cockroach ◽  
Promote Wound Healing ◽  
Fibroblast Migration

The American cockroach (Periplaneta americana) is a medicinal insect. Its extract is used clinically to promote wound healing and tissue regeneration, but the effective medicinal components and mechanisms are not yet clear. It has been reported that human thymosin beta 4 (Tβ4) may accelerate skin wound healing, however, the role of P. americana thymosin (Pa-THYs) is still poorly understood. In the present study, we identify and analyze the DNA sequences of Pa-THYs by bioinformatics analysis. Then we clone, express, and purify the Pa-THYs proteins and evaluate the activity of recombinant Pa-THYs proteins by cell migration and proliferation assays in NIH/3T3 cells. To elucidate the role of Pa-THYs in wound healing, a mouse model is established, and we evaluate wound contraction, histopathological parameters, and the expressions of several key growth factors after Pa-THYs treatment. Our results showed that three THY variants were formed by skipping splicing of exons. Pa-THYs could promote fibroblast migration, but have no effect on fibroblast proliferation. In wound repair, Pa-THYs proteins could effectively promote wound healing through stimulating dermal tissue regeneration, angiogenesis, and collagen deposition. On the molecular mechanism, Pa-THYs also stimulated the expression of several key growth factors to promote wound healing. The data suggest that Pa-THYs could be a potential drug for promoting wound repair.

scholarly journals Bioactivity-Guided Screening of Wound-Healing Active Constituents from American Cockroach (Periplaneta americana)

Molecules ◽  
2018 ◽  
Vol 23 (1) ◽  
pp. 101 ◽  
Author(s):  
Juan-Juan Zhu ◽  
Shun Yao ◽  
Xin Guo ◽  
Bi-Song Yue ◽  
Xiu-Ying Ma ◽  
...  
Keyword(s):  
Wound Healing ◽  
Periplaneta Americana ◽  
American Cockroach ◽  
Active Constituents
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