Cloning, Expression and Effects of P. americana Thymosin on Wound Healing

The American cockroach (Periplaneta americana) is a medicinal insect. Its extract is used clinically to promote wound healing and tissue regeneration, but the effective medicinal components and mechanisms are not yet clear. It has been reported that human thymosin beta 4 (Tβ4) may accelerate skin wound healing, however, the role of P. americana thymosin (Pa-THYs) is still poorly understood. In the present study, we identify and analyze the DNA sequences of Pa-THYs by bioinformatics analysis. Then we clone, express, and purify the Pa-THYs proteins and evaluate the activity of recombinant Pa-THYs proteins by cell migration and proliferation assays in NIH/3T3 cells. To elucidate the role of Pa-THYs in wound healing, a mouse model is established, and we evaluate wound contraction, histopathological parameters, and the expressions of several key growth factors after Pa-THYs treatment. Our results showed that three THY variants were formed by skipping splicing of exons. Pa-THYs could promote fibroblast migration, but have no effect on fibroblast proliferation. In wound repair, Pa-THYs proteins could effectively promote wound healing through stimulating dermal tissue regeneration, angiogenesis, and collagen deposition. On the molecular mechanism, Pa-THYs also stimulated the expression of several key growth factors to promote wound healing. The data suggest that Pa-THYs could be a potential drug for promoting wound repair.

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The Role of the Extracellular Matrix Components in Cutaneous Wound Healing

BioMed Research International ◽

10.1155/2014/747584 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 113

Author(s):

Pawel Olczyk ◽

Łukasz Mencner ◽

Katarzyna Komosinska-Vassev

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Growth Factors ◽

Wound Repair ◽

Structural Integrity ◽

Healing Process ◽

Cellular Functions ◽

Extracellular Matrix Components ◽

Essential Components ◽

Matrix Components

Wound healing is the physiologic response to tissue trauma proceeding as a complex pathway of biochemical reactions and cellular events, secreted growth factors, and cytokines. Extracellular matrix constituents are essential components of the wound repair phenomenon. Firstly, they create a provisional matrix, providing a structural integrity of matrix during each stage of healing process. Secondly, matrix molecules regulate cellular functions, mediate the cell-cell and cell-matrix interactions, and serve as a reservoir and modulator of cytokines and growth factors’ action. Currently known mechanisms, by which extracellular matrix components modulate each stage of the process of soft tissue remodeling after injury, have been discussed.

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The role of growth factors in extraction wound healing

Acta stomatologica Naissi ◽

10.5937/asn1572524s ◽

2015 ◽

Vol 31 (72) ◽

pp. 1524-1537

Author(s):

Simona Stojanovic ◽

Milos Tijanic ◽

Goran Jovanovic ◽

Milan Spasic ◽

Branislava Stojkovic ◽

...

Keyword(s):

Wound Healing ◽

Growth Factors

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Complements and the Wound Healing Cascade: An Updated Review

Plastic Surgery International ◽

10.1155/2013/146764 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 45

Author(s):

Hani Sinno ◽

Satya Prakash

Keyword(s):

Health Care ◽

Wound Healing ◽

Growth Factors ◽

Health Care System ◽

Complement Cascade ◽

Patient Morbidity ◽

Multistep Process ◽

The Usa ◽

Skin Healing

Wound healing is a complex pathway of regulated reactions and cellular infiltrates. The mechanisms at play have been thoroughly studied but there is much still to learn. The health care system in the USA alone spends on average 9 billion dollars annually on treating of wounds. To help reduce patient morbidity and mortality related to abnormal or prolonged skin healing, an updated review and understanding of wound healing is essential. Recent works have helped shape the multistep process in wound healing and introduced various growth factors that can augment this process. The complement cascade has been shown to have a role in inflammation and has only recently been shown to augment wound healing. In this review, we have outlined the biology of wound healing and discussed the use of growth factors and the role of complements in this intricate pathway.

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The Role of Growth Factors in the Regulation of Wound Repair

Host Defense Dysfunction in Trauma, Shock and Sepsis ◽

10.1007/978-3-642-77405-8_141 ◽

1993 ◽

pp. 1051-1064

Author(s):

D. R. Knighton ◽

V. D. Fiegel ◽

G. D. Phillips

Keyword(s):

Growth Factors ◽

Wound Repair

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Sequential Delivery of Cryogel Released Growth Factors and Cytokines Accelerates Wound Healing and Improves Tissue Regeneration

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2020.00345 ◽

2020 ◽

Vol 8 ◽

Cited By ~ 6

Author(s):

Shiro Jimi ◽

Alexandr Jaguparov ◽

Ayan Nurkesh ◽

Bolat Sultankulov ◽

Arman Saparov

Keyword(s):

Wound Healing ◽

Growth Factors ◽

Tissue Regeneration

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Abnormal Cell Responses and Role of TNF-αin Impaired Diabetic Wound Healing

BioMed Research International ◽

10.1155/2013/754802 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 53

Author(s):

Fanxing Xu ◽

Chenying Zhang ◽

Dana T. Graves

Keyword(s):

Wound Healing ◽

Macrophage Polarization ◽

Diabetic Wound ◽

Major Health Problem ◽

Abnormal Cell ◽

Fibroblast Migration ◽

Cell Responses ◽

Impaired Healing ◽

Diabetic Wound Healing

Impaired diabetic wound healing constitutes a major health problem. The impaired healing is caused by complex factors such as abnormal keratinocyte and fibroblast migration, proliferation, differentiation, and apoptosis, abnormal macrophage polarization, impaired recruitment of mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs), and decreased vascularization. Diabetes-enhanced and prolonged expression of TNF-αalso contributes to impaired healing. In this paper, we discuss the abnormal cell responses in diabetic wound healing and the contribution of TNF-α.

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