Bioassay of a nuclear-polyhedrosis virus and Bacillus thuringiensis against the American bollworm, Heliothis armigera, in Botswana

1974 ◽  
Vol 23 (3) ◽  
pp. 318-324 ◽  
Author(s):  
R.A. Daoust ◽  
R.E. Roome
Keyword(s):  
Bacillus Thuringiensis ◽  
Nuclear Polyhedrosis Virus ◽  
Heliothis Armigera ◽  
American Bollworm ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Field trials with a nuclear polyhedrosis virus and Bacillus thuringiensis against larvae of Heliothis armigera (Hb.) (Lepidoptera, Noctuidae) on sorghum and cotton in Botswana

1975 ◽  
Vol 65 (3) ◽  
pp. 507-514 ◽  
Author(s):  
R. E. Roome
Keyword(s):  
Bacillus Thuringiensis ◽  
Nuclear Polyhedrosis Virus ◽  
Heliothis Armigera ◽  
Field Trials ◽  
Natural Spread ◽  
Low Concentrations ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Lepidoptera Noctuidae ◽  
Very High

AbstractSix trials with an unpurified suspension of Heliothis armigera nuclear polyhedrosis virus (NPV) against Heliothis armigera (Hb.) larvae on sorghum (five trials) and on cotton (one trial) during 1971–73 are described. Commercial preparations of H. zea NPV and of Bacillus thuringiensis were included in two of the trials. H. armigera NPV was as successful as a standard insecticide in preventing losses of sorghum in one trial. Lowering the rate of NPV reduced the control of H. armigera larvae. There was no difference in control achieved by spraying in the morning or in the evening. Spraying every second or every third row (relying on natural spread of the virus) reduced control. Low concentrations of molasses (0·6%) made control by 100 larval equivalents (L.E.) per hectare as good as that by 200 L.E. per hectare without molasses. Neither the commercial H. zea NPV nor the Bacillus preparation was as effective as the local virus. The local virus was reasonably effective against H. armigera on cotton at very high rates and with molasses. It was not as damaging as carbaryl to populations of egg parasites and predators, but the predators did not control damaging populations of aphid and Jassid on the virus-sprayed plots.

scholarly journals Laboratorium Sederhana Perbanyakan Agensia Hayati Pengendali Hama dan Penyakit Tanaman Padi di PP Gapsera Sejahtera Madiri Lampung Tengah

2020 ◽  
Vol 1 (1) ◽  
pp. 73-79
Author(s):  
- Ivayani
Keyword(s):  
Bacillus Thuringiensis ◽  
Nuclear Polyhedrosis Virus ◽  
Air Flow ◽  
Polyhedrosis Virus ◽  
Laminar Air Flow ◽  
Nuclear Polyhedrosis

Kecamatan Seputih Raman Kabupaten Lampung Tengah merupakan salah satu daerah penghasil komoditi padi yang memasok persediaan beras di Provinsi Lampung. Gapoktan Gapsera sejahtera Mandiri merupakan salah satu Gapoktan di Kecamatan Seputih Raman yang sudah menerapkan pertanian organik dalam budidaya tanamana padi. Salah satu produk yang dihasilkan oleh Gapoktan Subur Asri adalah beras organik “Berasera”. Dalam proses budidaya dan pengendalian hama peyakit Gapoktan Subur Asri sudah tidak menggunakan bahan kimia sintetik melainkan dengan menggunakan agensia hayati. Akan tetapi dalam perbanyakan agensia hayati (mikroorganisme) belum maksimal karena kurangnya pengetahuan mengenai teknik perbanyakan agensia hayati, selain itu juga tidak tersedianya tempat kerja perbanyakan yang tidak aseptik (steril) sehingga terjadinya kontaminasi dengan mikroorganisme lainnya. Oleh karena itu untuk menciptakan lingkungan perbanyakan agensia hayati yang aseptik maka diperlukan suatu Laboratorium sederhana yang terdiri dari alat-alat standar Laboratorium. Metode yang digunakan adalah pembimbingan melalui sosialisasi teknik perbanyakan agensia hayati, pendampingan dalam mencari agensia hayati di lapangan dan pendampingan pembuatan laboratorium sederhana. Kegiatan yang telah dilaksanakan di PP Gapsera Sejahtera Mandiri adalah Penyuluhan mengenai cara isolasi agensia hayati NPV (Nuclear Polyhedrosis Virus) dan isolasi Bt (Bacillus thuringiensis) serta teknik perbanyakannya, Teknik bekerja secara aseptik dan Pembuatan Laboratorium sederhana yang salah satu alatnya ialah Enkas (Lemari kerja aseptik) / Laminar air flow. Berdasarkan kegiatan yang telah dilakukan, petani mampu melakukan isolasi NPV dan Bt, serta mampu bekerja secara aseptik guna mendapatkan/ memperbanyak agensia hayati pengendali hama dan penyakit tumbuhan. Luaran dari kegiatan ini adalah tersedianya laboratorium sederhana yang operasioanal untuk perbanyakan agensia hayati pengendali hama dan penyakit tanaman.

In vivo Production of a Nuclear Polyhedrosis Virus Utilizing Tobacco Budworm and a Multicellular Larval Rearing Container2

1991 ◽  
Vol 26 (1) ◽  
pp. 69-75 ◽  
Author(s):  
M. R. Bell
Keyword(s):  
Heliothis Virescens ◽  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Heliothis Armigera ◽  
Tobacco Budworm ◽  
Larval Rearing ◽  
Average Production ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The effects of viral inoculum and incubation period on virus yield in a method of in vivo production of the multicapsid nuclear polyhedrosis virus of Heliothis armigera (Hübner) were examined utilizing Heliothis virescens larvae reared in multicellular containers. Trays of diet were surface contaminated with virus levels ranging from 54 to 2708 polyhedral inclusion bodies (PIB) per mm2 of diet at 7 days after egg infestation. The virus was harvested either at 6 or 7 days after treatment. Within the ranges of this study, the greatest quantity virus was obtained when the diet was inoculated with the 54 PIB level, and the virus was harvested at 7 days. This combination resulted in an average production of 2919 × 109 PIB per tray, with 5.7 × 109 PIB per larvae and 523 collectable larvae per tray. It also represented an increase in polyhedra of 2.43 × 105× compared to the inoculum. The costs of the production were estimated based on prevailing wages and current costs of materials.

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