Dosage-mortality studies of a granulosis and a nuclear polyhedrosis virus of a laboratory strain of Heliothis armigera

1978 ◽  
Vol 32 (3) ◽  
pp. 386-387 ◽  
Author(s):  
V.H. Whitlock
Keyword(s):  
Nuclear Polyhedrosis Virus ◽  
Laboratory Strain ◽  
Heliothis Armigera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

In vivo Production of a Nuclear Polyhedrosis Virus Utilizing Tobacco Budworm and a Multicellular Larval Rearing Container2

1991 ◽  
Vol 26 (1) ◽  
pp. 69-75 ◽  
Author(s):  
M. R. Bell
Keyword(s):  
Heliothis Virescens ◽  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Heliothis Armigera ◽  
Tobacco Budworm ◽  
Larval Rearing ◽  
Average Production ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The effects of viral inoculum and incubation period on virus yield in a method of in vivo production of the multicapsid nuclear polyhedrosis virus of Heliothis armigera (Hübner) were examined utilizing Heliothis virescens larvae reared in multicellular containers. Trays of diet were surface contaminated with virus levels ranging from 54 to 2708 polyhedral inclusion bodies (PIB) per mm2 of diet at 7 days after egg infestation. The virus was harvested either at 6 or 7 days after treatment. Within the ranges of this study, the greatest quantity virus was obtained when the diet was inoculated with the 54 PIB level, and the virus was harvested at 7 days. This combination resulted in an average production of 2919 × 109 PIB per tray, with 5.7 × 109 PIB per larvae and 523 collectable larvae per tray. It also represented an increase in polyhedra of 2.43 × 105× compared to the inoculum. The costs of the production were estimated based on prevailing wages and current costs of materials.

scholarly journals Control of Heliothis armigera in Maize with a Nuclear Polyhedrosis Virus

1989 ◽  
Vol 24 (2) ◽  
pp. 186-192 ◽  
Author(s):  
Shu-Jen TUAN ◽  
Li-Cheng TANG ◽  
Roger Feng-Nan HOU
Keyword(s):  
Nuclear Polyhedrosis Virus ◽  
Heliothis Armigera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Field trials with a nuclear polyhedrosis virus and Bacillus thuringiensis against larvae of Heliothis armigera (Hb.) (Lepidoptera, Noctuidae) on sorghum and cotton in Botswana

1975 ◽  
Vol 65 (3) ◽  
pp. 507-514 ◽  
Author(s):  
R. E. Roome
Keyword(s):  
Bacillus Thuringiensis ◽  
Nuclear Polyhedrosis Virus ◽  
Heliothis Armigera ◽  
Field Trials ◽  
Natural Spread ◽  
Low Concentrations ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Lepidoptera Noctuidae ◽  
Very High

AbstractSix trials with an unpurified suspension of Heliothis armigera nuclear polyhedrosis virus (NPV) against Heliothis armigera (Hb.) larvae on sorghum (five trials) and on cotton (one trial) during 1971–73 are described. Commercial preparations of H. zea NPV and of Bacillus thuringiensis were included in two of the trials. H. armigera NPV was as successful as a standard insecticide in preventing losses of sorghum in one trial. Lowering the rate of NPV reduced the control of H. armigera larvae. There was no difference in control achieved by spraying in the morning or in the evening. Spraying every second or every third row (relying on natural spread of the virus) reduced control. Low concentrations of molasses (0·6%) made control by 100 larval equivalents (L.E.) per hectare as good as that by 200 L.E. per hectare without molasses. Neither the commercial H. zea NPV nor the Bacillus preparation was as effective as the local virus. The local virus was reasonably effective against H. armigera on cotton at very high rates and with molasses. It was not as damaging as carbaryl to populations of egg parasites and predators, but the predators did not control damaging populations of aphid and Jassid on the virus-sprayed plots.

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