Effects of oxidatively modified low density lipoprotein (LDL) cholesterol on cultured smooth muscle cells *1Kanzhi Liu, Bram Ramjiawan, Michael J.B. Kutryk and Grant N. Pierce. Division of Cardiovascular Sciences, St. Boniface Research Centre, Winnipeg, Canad R2H 2A6

1990 ◽  
Vol 22 ◽  
pp. S49
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Ldl Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Muscle Cells ◽  
Research Centre ◽  
Low Density ◽  
Cultured Smooth Muscle Cells ◽  
Oxidatively Modified

Oxidized low-density lipoprotein induces cytoskeletal disorganization in smooth muscle cells

1999 ◽  
Vol 277 (5) ◽  
pp. H2017-H2025 ◽  
Author(s):  
Hamid Massaeli ◽  
Cecilia Hurtado ◽  
J. Alejandro Austria ◽  
Grant N. Pierce
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Oxidized Ldl ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Muscle Cells ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein ◽  
Cultured Smooth Muscle Cells ◽  
Actin Protein

Vascular smooth muscle cells in atherosclerotic vessels proliferate and change from a contractile to a synthetic phenotype. To determine whether oxidized low-density lipoprotein (oxLDL) is involved in this transformation, we chronically incubated cultured smooth muscle cells with native and oxidized LDL. Western blot analysis detected a decrease in actin and myosin content in treated cells. This was dependent on the time and concentration of oxLDL employed. Confocal microscopic images of cells immunostained for smooth muscle-specific α-actin and myosin showed a normal, elongated alignment of myofilaments in cells after incubation with native LDL. Surprisingly, when the cells were treated with oxLDL, actin and myosin filaments underwent a striking process of disorganization and accumulation into ball-shaped aggregates. These changes were dependent on the duration and concentration of oxLDL employed. Our results demonstrate that oxLDL has the capacity to decrease the content of myofilaments in smooth muscle cells. The loss in myosin and actin protein may be associated with an unusual formation of large cellular aggregates that appear to be in the process of being expelled from the cell.

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