DNA synthesis in bacteriophage T4-infected Escherichia coli: Evidence supporting a stoichiometric role for gene 32-product

1971 ◽  
Vol 62 (1) ◽  
pp. 267-271 ◽  
Author(s):  
Navin K. Sinha ◽  
D.Peter Snustad
Keyword(s):  
Escherichia Coli ◽  
Dna Synthesis ◽  
Bacteriophage T4 ◽  
Gene 32

Molecular Aspects of Gene 32 Expression in Escherichia coli Infected with the Bacteriophage T4

1977 ◽  
pp. 91-113 ◽  
Author(s):  
LARRY GOLD ◽  
GENEVIEVE LEMAIRE ◽  
CHRISTOPHER MARTIN ◽  
HOPE MORRISSETT ◽  
PAMELA O'CONNER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage T4 ◽  
Expression In Escherichia Coli ◽  
Molecular Aspects ◽  
Gene 32

Host DNA synthesis after infection of Escherichia coli with mutants of bacteriophage T4

Virology ◽  
1971 ◽  
Vol 46 (2) ◽  
pp. 437-452 ◽  
Author(s):  
A. Cascino ◽  
S. Riva ◽  
E.P. Geiduschek
Keyword(s):  
Escherichia Coli ◽  
Dna Synthesis ◽  
Bacteriophage T4

scholarly journals Studies on the Recombination Genes of Bacteriophage T4: Suppression of uvsX and uvsY Mutations by uvsW Mutations

Genetics ◽  
1987 ◽  
Vol 115 (2) ◽  
pp. 219-227
Author(s):  
Tetsuro Yonesaki ◽  
Teiichi Minagawa
Keyword(s):  
Molecular Weight ◽  
Dna Synthesis ◽  
Burst Size ◽  
Bacteriophage T4 ◽  
Uv Resistance ◽  
Small Plaque ◽  
New Mutation ◽  
Single Stranded Dna ◽  
Large Plaque ◽  
Gene 32

ABSTRACT Genes uvsW, uvsX and uvsY are dispensable for T4 growth but are implicated in recombination and in the repair of damaged DNA. We found that large-plaque mutants arose efficiently from small-plaque uvsX and uvsY mutants at 42° and were pseudorevertants containing a new mutation in uvsW. Using reconstructed double mutants, we confirmed that a mutation in uvsW partially increases the burst size and UV resistance of uvsX and uvsY mutants. At 41° the uvsW mutation completely restores the arrest in DNA synthesis caused by mutations in genes uvsX, uvsY and 46, but at 30° it only partially restores DNA synthesis in a gene 46 mutant and does not restore DNA synthesis in uvsX and uvsY mutants. Restored DNA synthesis at 41° was paralleled by the overproduction of single-stranded DNA and gene 32 protein. Based on these findings, we propose that the uvsW gene regulates the production of single-stranded DNA and we discuss the phenotype of uvsW mutants and their suppression of some uvsX and uvsY phenotypes. Infection of restrictive cells with am uvsW mutants revealed a defect in the synthesis of a protein of molecular weight 53,000 daltons, suggesting that this protein is the uvsW gene product.

Sign in / Sign up

Export Citation Format

Share Document