Identification of several proteins involved in the messenger RNA binding site of the 30 S ribosome by inactivation with 2-methoxy-5-nitrotropone

1977 ◽  
Vol 117 (2) ◽  
pp. 401-418 ◽  
Author(s):  
Chu Chang ◽  
Gary R. Craven
Keyword(s):  
Binding Site ◽  
Messenger Rna ◽  
Rna Binding

scholarly journals The proteins of the messenger RNA binding site of Escherichia coli ribosomes

1981 ◽  
Vol 9 (14) ◽  
pp. 3465-3481 ◽  
Author(s):  
O.I. Gimautdinova ◽  
G.G. Karpova ◽  
D.G. Knorre ◽  
N.D. Kobetz
Keyword(s):  
Escherichia Coli ◽  
Binding Site ◽  
Messenger Rna ◽  
Rna Binding

scholarly journals Asymmetric reconstruction of mammalian reovirus reveals interactions among RNA, transcriptional factor µ2 and capsid proteins

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Muchen Pan ◽  
Ana L. Alvarez-Cabrera ◽  
Joon S. Kang ◽  
Lihua Wang ◽  
Chunhai Fan ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Molecular Interactions ◽  
Messenger Rna ◽  
Rna Binding ◽  
Capsid Proteins ◽  
Rna Transcription ◽  
Infectious Particle ◽  
Virion Assembly ◽  
Rna Capping

AbstractMammalian reovirus (MRV) is the prototypical member of genus Orthoreovirus of family Reoviridae. However, lacking high-resolution structures of its RNA polymerase cofactor μ2 and infectious particle, limits understanding of molecular interactions among proteins and RNA, and their contributions to virion assembly and RNA transcription. Here, we report the 3.3 Å-resolution asymmetric reconstruction of transcribing MRV and in situ atomic models of its capsid proteins, the asymmetrically attached RNA-dependent RNA polymerase (RdRp) λ3, and RdRp-bound nucleoside triphosphatase μ2 with a unique RNA-binding domain. We reveal molecular interactions among virion proteins and genomic and messenger RNA. Polymerase complexes in three Spinoreovirinae subfamily members are organized with different pseudo-D3d symmetries to engage their highly diversified genomes. The above interactions and those between symmetry-mismatched receptor-binding σ1 trimers and RNA-capping λ2 pentamers balance competing needs of capsid assembly, external protein removal, and allosteric triggering of endogenous RNA transcription, before, during and after infection, respectively.

Messenger-RNA-binding proteins and the messages they carry

2002 ◽  
Vol 3 (3) ◽  
pp. 195-205 ◽  
Author(s):  
Gideon Dreyfuss ◽  
V. Narry Kim ◽  
Naoyuki Kataoka
Keyword(s):  
Messenger Rna ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins

Mutational and Structural Analysis of the RNA Binding Site for Escherichia coli Ribosomal Protein S7

1994 ◽  
Vol 244 (1) ◽  
pp. 74-85 ◽  
Author(s):  
François Dragon ◽  
Catherine Payant ◽  
Léa Brakier-Gingras
Keyword(s):  
Escherichia Coli ◽  
Structural Analysis ◽  
Ribosomal Protein ◽  
Binding Site ◽  
Rna Binding

Mutant Ribosomal Protein with Defective RNA Binding Site

1971 ◽  
Vol 234 (52) ◽  
pp. 273-275 ◽  
Author(s):  
M. GREEN ◽  
C. G. KURLAND
Keyword(s):  
Ribosomal Protein ◽  
Binding Site ◽  
Rna Binding ◽  
Defective Rna
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