scholarly journals Crystallization of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10

1988 ◽  
Vol 200 (3) ◽  
pp. 611-612 ◽  
Author(s):  
Henriëtte J. Rozeboom ◽  
Jaap Kingma ◽  
Dick B. Janssen ◽  
Bauke W. Dijkstra
Keyword(s):  
Haloalkane Dehalogenase ◽  
Xanthobacter Autotrophicus

Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 refined at 1.15 Å resolution

1999 ◽  
Vol 55 (7) ◽  
pp. 1273-1290 ◽  
Author(s):  
Ivo S. Ridder ◽  
Henriëtte J. Rozeboom ◽  
Bauke W. Dijkstra
Keyword(s):  
Main Chain ◽  
Chloride Ions ◽  
Water Molecules ◽  
Haloalkane Dehalogenase ◽  
Hydrogen Bond Acceptor ◽  
Peptide Bonds ◽  
Mean Values ◽  
Xanthobacter Autotrophicus ◽  
Covalently Linked ◽  
Multiple Conformations

Crystals of the 35 kDa protein haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 diffract to 1.15 Å resolution at cryogenic temperature using synchrotron radiation. Blocked anisotropic least-squares refinement with SHELXL gave a final conventional R factor of 10.51% for all reflections in the 15–1.15 Å resolution range. The estimated r.m.s. errors of the model are 0.026 and 0.038 Å for protein atoms and all atoms, respectively. The structure comprises all 310 amino acids, with 28 side chains and two peptide bonds in multiple conformations, two covalently linked Pb atoms, 601 water molecules, seven glycerol molecules, one sulfate ion and two chloride ions. Water molecules accounting for alternative solvent structure are modelled with a fixed occupancy of 0.5. The structure is described in detail and compared with previously reported dehalogenase structures refined at 1.9–2.3 Å resolution. An analysis of the protein's geometry and stereochemistry reveals eight mean values of bond lengths and angles which deviate significantly from the Engh & Huber parameters, a wide spread in the main-chain ω torsion angle around its ideal value of 180 (6)° and a role for C—H\cdotsO interactions in satisfying the hydrogen-bond acceptor capacity of main-chain carbonyl O atoms in the central β-sheet.

Expression of glycosylated haloalkane dehalogenase LinB in Pichia pastoris

2006 ◽  
Vol 46 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Takashi Nakamura ◽  
Marcel Zámocký ◽  
Zbyněk Zdráhal ◽  
Radka Chaloupková ◽  
Marta Monincová ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Haloalkane Dehalogenase

scholarly journals Histidine 289 Is Essential for Hydrolysis of the Alkyl-enzyme Intermediate of Haloalkane Dehalogenase

1995 ◽  
Vol 270 (18) ◽  
pp. 10405-10411 ◽  
Author(s):  
Frens Pries ◽  
Jaap Kingma ◽  
Geja H. Krooshof ◽  
C. Margot Jeronimus-Stratingh ◽  
Andries P. Bruins ◽  
...  
Keyword(s):  
Haloalkane Dehalogenase ◽  
Hydrolysis Of ◽  
Enzyme Intermediate

scholarly journals Sequence similarity of mammalian epoxide hydrolases to the bacterial haloalkane dehalogenase and other related proteins

FEBS Letters ◽  
1994 ◽  
Vol 338 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Michael Arand ◽  
David F. Grant ◽  
Jeffrey K. Beetham ◽  
Thomas Friedberg ◽  
Franz Oesch ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Haloalkane Dehalogenase ◽  
Epoxide Hydrolases ◽  
Related Proteins

X-ray crystal structure of Mycobacterium tuberculosis haloalkane dehalogenase Rv2579

2008 ◽  
Vol 1784 (2) ◽  
pp. 351-362 ◽  
Author(s):  
Pooja A. Mazumdar ◽  
Jordan C. Hulecki ◽  
Maia M. Cherney ◽  
Craig R. Garen ◽  
Michael N.G. James
Keyword(s):  
Crystal Structure ◽  
Mycobacterium Tuberculosis ◽  
Haloalkane Dehalogenase ◽  
X Ray

The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: Reaction mechanism and molecular evolution

2007 ◽  
Vol 157 (2) ◽  
pp. 384-392 ◽  
Author(s):  
Martina Pavlová ◽  
Martin Klvaňa ◽  
Andrea Jesenská ◽  
Zbyněk Prokop ◽  
Hana Konečná ◽  
...  
Keyword(s):  
Reaction Mechanism ◽  
Molecular Evolution ◽  
Mycobacterium Avium ◽  
Haloalkane Dehalogenase
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