scholarly journals Adaptation of Xanthobacter autotrophicus GJ10 to bromoacetate due to activation and mobilization of the haloacetate dehalogenase gene by insertion element IS1247.

1995 ◽  
Vol 177 (5) ◽  
pp. 1348-1356 ◽  
Author(s):  
J van der Ploeg ◽  
M Willemsen ◽  
G van Hall ◽  
D B Janssen
Keyword(s):  
Insertion Element ◽  
Xanthobacter Autotrophicus ◽  
Dehalogenase Gene

Insertion sequence IST3091 of Thiobacillus ferrooxidans

1997 ◽  
Vol 43 (6) ◽  
pp. 503-508 ◽  
Author(s):  
Leena Chakravarty ◽  
Joseph D. Kittle Jr. ◽  
Olli H. Tuovinen
Keyword(s):  
Insertion Sequence ◽  
Thiobacillus Ferrooxidans ◽  
Insertion Element ◽  
Transposase Gene ◽  
Integrase Gene ◽  
Spiroplasma Citri ◽  
E Coli ◽  
Putative Origin ◽  
Replication Region ◽  
Polymerase Chain

An insertion sequence, designated as IST3091, was located adjacent to the putative origin of replication region of plasmid pTFI91 of Thiobacillus ferrooxidans TFI-91. The DNA sequence of the transposase gene of IST3091 revealed similarity with that of IS30, IS1086, IS4351, and the integrase gene of SpV1-R8A2 B (a bacteriophage of Spiroplasma citri). The sequence of IST3091 is 1063 bp long with partially matched 30-bp terminal inverted repeats. Several restriction fragments of plasmid pTFI91 of T. ferrooxidans containing the IST3091 element were cloned into the vector pHSG398. The hybrid plasmids (pBTL) were transformed into Escherichia coli NK7379 containing a miniF plasmid, which was devoid of transposable elements. The transposition function of the IST3091 element was confirmed by mobilizing hybrid plasmids via conjugation from transformed E. coli NK7379 (donor) to E. coli M8820 (recipient). The presence of the transposed element in transconjugants was detected by polymerase chain reaction amplification.Key words: insertion element, Thiobacillus ferrooxidans, transformation, transposase.

scholarly journals Genomic distribution of a novel Pyrenophora tritici-repentis ToxA insertion element

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206586 ◽  
Author(s):  
Paula M. Moolhuijzen ◽  
Pao Theen See ◽  
Richard P. Oliver ◽  
Caroline S. Moffat
Keyword(s):  
Insertion Element ◽  
Genomic Distribution ◽  
Pyrenophora Tritici Repentis

Mapping of insertion element IS30 in theEscherichia coli K12 chromosome

1990 ◽  
Vol 222 (2-3) ◽  
pp. 317-322 ◽  
Author(s):  
Masaaki Umeda ◽  
Efchi Ohtsubo
Keyword(s):  
Insertion Element
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