Cloning and sequencing of cDNA clones encoding chicken lamins A and B1 and comparison of the primary structures of vertebrate A- and B-type lamins

1989 ◽  
Vol 208 (3) ◽  
pp. 393-404 ◽  
Author(s):  
M. Peter ◽  
G.T. Kitten ◽  
C.F. Lehner ◽  
K. Vorburger ◽  
S.M. Bailer ◽  
...  
Keyword(s):  
Cdna Clones ◽  
Cloning And Sequencing ◽  
Primary Structures

scholarly journals Different molecular forms of rat kidney gp330, the dominant autoantigen of active Heymann nephritis

1995 ◽  
Vol 305 (3) ◽  
pp. 711-713 ◽  
Author(s):  
G G Jokhadze ◽  
A V Oleinikov ◽  
J J Kanalas ◽  
S P Makker
Keyword(s):  
Amino Acid ◽  
Direct Evidence ◽  
Rat Kidney ◽  
Single Amino Acid ◽  
Molecular Forms ◽  
Cdna Clones ◽  
Amino Acid Residues ◽  
Cloning And Sequencing ◽  
Heymann Nephritis ◽  
Primary Structures

The primary structure, consisting of 1650 amino acid residues, of the C-terminal end of the dominant autoantigen of active Heymann Nephritis, gp330, from rat kidney was obtained by cloning and sequencing of cDNA clones. Comparison of this sequence with the previously published sequences of fragments of the C-terminal end of gp330 [Raychowdhury, Niles, McCluskey and Smith (1989) Science 244, 1163-1165] revealed certain differences in their primary structures. These differences included several single amino acid substitutions, replacement of a stretch of 15 amino acid residues by a different stretch of six amino acid residues, and different lengths of cytoplasmic domain (188 versus 213 amino acid residues). These findings of two different primary structures of gp330 provide direct evidence for the existence of two molecular forms of gp330.

scholarly journals Cloning and sequencing of protochlorophyllide reductase

1990 ◽  
Vol 265 (3) ◽  
pp. 789-798 ◽  
Author(s):  
P M Darrah ◽  
S A Kay ◽  
G R Teakle ◽  
W T Griffiths
Keyword(s):  
Amino Acid ◽  
Chemical Analysis ◽  
Amino Acid Sequence ◽  
Cdna Library ◽  
Avena Sativa ◽  
Cdna Clones ◽  
Lambda Phage ◽  
Cloning And Sequencing ◽  
Protochlorophyllide Reductase ◽  
Cnbr Cleavage

Putative protochlorophyllide reductase cDNA clones (252 and 113) were isolated from an etiolated-oat (Avena sativa) cDNA library. These were used to indirectly characterize a further clone, p127, isolated from a lambda-phage gt11 cDNA library. The latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a CNBr-cleavage fragment of the purified reductase, p127 codes for more than 95% of the reductase protein.

scholarly journals Primary Structures of Arabidopsis Calmodulin Isoforms Deduced from the Sequences of cDNA Clones

1991 ◽  
Vol 96 (4) ◽  
pp. 1196-1202 ◽  
Author(s):  
Vincent Ling ◽  
Imara Perera ◽  
Raymond E. Zielinski
Keyword(s):  
Cdna Clones ◽  
Primary Structures

scholarly journals Barley arabinoxylan arabinofuranohydrolases: purification, characterization and determination of primary structures from cDNA clones

2001 ◽  
Vol 356 (1) ◽  
pp. 181 ◽  
Author(s):  
Robert C. LEE ◽  
Rachel A. BURTON ◽  
Maria HRMOVA ◽  
Geoffrey B. FINCHER
Keyword(s):  
Cdna Clones ◽  
Primary Structures

Monitoring the marine environment for small round structured viruses (SRSVS): a new approach to combating the transmission of these viruses by molluscan shellfish

1998 ◽  
Vol 38 (12) ◽  
pp. 51-56 ◽  
Author(s):  
K. Henshilwood ◽  
J. Green ◽  
D. N. Lees
Keyword(s):  
Enteric Virus ◽  
Winter Period ◽  
Rt Pcr ◽  
Cloning And Sequencing ◽  
New Approach ◽  
Human Enteric Virus ◽  
Different Strains ◽  
The Uk ◽  
Public Health Protection

This study investigates human enteric virus contamination of a shellfish harvesting area. Samples were analysed over a 14-month period for Small Round Structured Viruses (SRSVs) using a previously developed nested RT-PCR. A clear seasonal difference was observed with the largest numbers of positive samples obtained during the winter period (October to March). This data concurs with the known winter association of gastroenteric illness due to oyster consumption in the UK and also with the majority of the outbreaks associated with shellfish harvested from this area during the study period. RT-PCR positive amplicons were further characterised by cloning and sequencing. Sequence analysis of the positive samples identified eleven SRSV strains, of both Genogroup I and Genogroup II, occurring throughout the study period. Many shellfish samples contained a mixture of strains with a few samples containing up to three different strains with both Genogroups represented. The observed common occurrence of strain mixtures may have implications for the role of shellfish as a vector for dissemination of SRSV strains. These results show that nested RT-PCR can identify SRSV contamination in shellfish harvesting areas. Virus monitoring of shellfish harvesting areas by specialist laboratories using RT-PCR is a possible approach to combating the transmission of SRSVs by molluscan shellfish and could potentially offer significantly enhanced levels of public health protection.

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