Demyelinating, non-demyelinating and attenuated canine distemper virus strains induce oligodendroglial cytolysis in vitro

Virulence of canine distemper virus (CDV) adapted to in vitro growth in Vero or bovine cells was determined by inoculation into CDV-susceptible neonatal gnotobiotic dogs. When compared with dogs given virulent R252-CDV, Vero R252-CDV was attenuated at passage level 14. In contrast, dogs inoculated with bovine R252-CDV at the same passage level experienced rapid fatal neurological disease. Virulence was not linked to ability to infect or replicate in canine pulmonary macrophage cultures. Retention of virulence by bovine R252-CDV is unique and worthy of further study.

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Virulent and attenuated canine distemper virus infects multiple dog brain cell types in vitro

Glia ◽

10.1002/glia.440040409 ◽

1991 ◽

Vol 4 (4) ◽

pp. 408-416 ◽

Cited By ~ 14

Author(s):

Susan Pearce-Kelling ◽

William J. Mitchell ◽

Brian A. Summers ◽

Max J. G. Appel

Keyword(s):

Canine Distemper Virus ◽

Cell Types ◽

Brain Cell ◽

Canine Distemper ◽

Dog Brain ◽

Brain Cell Types

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Canine distemper virus-induced glial cell changes in vitro

Acta Neuropathologica ◽

10.1007/bf00684920 ◽

1983 ◽

Vol 62 (1-2) ◽

pp. 51-58 ◽

Cited By ~ 12

Author(s):

A. Zurbriggen ◽

M. Vandevelde

Keyword(s):

Glial Cell ◽

Canine Distemper Virus ◽

Canine Distemper ◽

Cell Changes

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Complement-Mediated Neutralization of Canine Distemper Virus In Vitro: Cross-Reaction between Vaccine Onderstepoort and Field KDK-1 Strains with Different Hemagglutinin Gene Characteristics

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.4.921-924.2002 ◽

2002 ◽

Vol 9 (4) ◽

pp. 921-924 ◽

Cited By ~ 2

Author(s):

Masami Mochizuki ◽

Megumi Motoyoshi ◽

Ken Maeda ◽

Kazunari Kai

Keyword(s):

Guinea Pig ◽

Canine Distemper Virus ◽

Field Isolate ◽

Neutralization Assay ◽

Cross Reaction ◽

Canine Distemper ◽

Hemagglutinin Gene

ABSTRACT The properties of neutralization of antigens of canine distemper virus Onderstepoort and a recent field isolate, KDK-1, were investigated with strain-specific dog sera. A conventional neutralization assay indicated antigenic dissimilarity between the strains; however, when guinea pig complement was included in the reaction mixture, the strains were neutralized with not only the homologous but also the heterologous antibodies.

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Recovery of NanoLuc Luciferase-Tagged Canine Distemper Virus for Facilitating Rapid Screening of Antivirals in vitro

Frontiers in Veterinary Science ◽

10.3389/fvets.2020.600796 ◽

2020 ◽

Vol 7 ◽

Author(s):

Fuxiao Liu ◽

Qianqian Wang ◽

Yilan Huang ◽

Ning Wang ◽

Youming Zhang ◽

...

Keyword(s):

High Throughput ◽

High Throughput Screening ◽

Canine Distemper Virus ◽

Reverse Genetics ◽

Rapid Screening ◽

Canine Distemper ◽

Virus Propagation ◽

The Family ◽

Conventional Methods

Canine distemper virus (CDV), belonging to the genus Morbillivirus in the family Paramyxoviridae, is a highly contagious pathogen, affecting various domestic, and wild carnivores. Conventional methods are too cumbersome to be used for high-throughput screening of anti-CDV drugs. In this study, a recombinant CDV was rescued using reverse genetics for facilitating screening of anti-CDV drug in vitro. The recombinant CDV could stably express the NanoLuc® luciferase (NLuc), a novel enzyme that was smaller and “brighter” than others. The intensity of NLuc-catalyzed luminescence reaction indirectly reflected the anti-CDV effect of a certain drug, due to a positive correlation between NLuc expression and virus propagation in vitro. Based on such a characteristic feature, the recombinant CDV was used for anti-CDV assays on four drugs (ribavirin, moroxydine hydrochloride, 1-adamantylamine hydrochloride, and tea polyphenol) via analysis of luciferase activity, instead of via conventional methods. The result showed that out of these four drugs, only the ribavirin exhibited a detectable anti-CDV effect. The NLuc-tagged CDV would be a rapid tool for high-throughput screening of anti-CDV drugs.

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