Properties of R-plasmid pEB017, which confers both enhanced UV-radiation resistance and mutability to wild-type, recA and umuC strains of Escherichia coli K12

1992 ◽  
Vol 267 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Emmanuel E. Obaseiki-Ebor ◽  
Kendric C. Smith
Keyword(s):  
Escherichia Coli ◽  
Uv Radiation ◽  
Radiation Resistance ◽  
Wild Type ◽  
Escherichia Coli K12

scholarly journals Modulation of DNA Repair and Recombination by the Bacteriophage λ Orf Function in Escherichia coli K-12

2004 ◽  
Vol 186 (9) ◽  
pp. 2699-2707 ◽  
Author(s):  
Anthony R. Poteete
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Uv Radiation ◽  
Bacteriophage Λ ◽  
Wild Type ◽  
Uv Sensitivity ◽  
Mutant Phenotypes ◽  
K 12 ◽  
Related Plasmid

ABSTRACT The orf gene of bacteriophage λ, fused to a promoter, was placed in the galK locus of Escherichia coli K-12. Orf was found to suppress the recombination deficiency and sensitivity to UV radiation of mutants, in a Δ(recC ptr recB recD)::P tac gam bet exo pae cI ΔrecG background, lacking recF, recO, recR, ruvAB, and ruvC functions. It also suppressed defects of these mutants in establishing replication of a pSC101-related plasmid. Compared to orf, the recA803 allele had only small effects on recF, recO, and recR mutant phenotypes and no effect on a ruvAB mutant. In a fully wild-type background with respect to known recombination and repair functions, orf partially suppressed the UV sensitivity of ruvAB and ruvC mutants.

Sequence of the mglB gene from Escherichia coli K12: Comparison of wild-type and mutant galactose chemoreceptors

1987 ◽  
Vol 208 (1-2) ◽  
pp. 247-253 ◽  
Author(s):  
Annette Scholle ◽  
Jörg Vreemann ◽  
Volker Blank ◽  
Annette Nold ◽  
Winfried Boos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Wild Type ◽  
Escherichia Coli K12

scholarly journals Galactose-specific messenger ribonucleic acid contents in Escherichia coli

1971 ◽  
Vol 121 (1) ◽  
pp. 109-116 ◽  
Author(s):  
J. R. Gosden ◽  
M. I. Irving ◽  
J. O. Bishop
Keyword(s):  
Escherichia Coli ◽  
Ribonucleic Acid ◽  
Gene Frequency ◽  
Wild Type ◽  
Total Rna ◽  
Messenger Ribonucleic Acid ◽  
E Coli ◽  
Escherichia Coli K12 ◽  
Different Cultures ◽  
Specific Mrna

A method is described for measuring the proportion of galactose-specific mRNA (gal-mRNA) in the total RNA extracted from pulse-labelled cells of Escherichia coli K12, by DNA–RNA hybridization with DNA prepared from bacteriophage λdg. RNA from wild-type E. coli was compared with RNA from a homogenote carrying the gal operon both in the chromosome and in a substituted sex-factor, and with RNA from a deletion strain that carried the galactose operon only in the exogenote. In each case the cultures were induced with fucose. Under these conditions the amount of gal-mRNA was found to be proportional to the content of galactokinase in the different cultures, and to the gene frequency. The amounts of gal-mRNA in an Oc mutant and an R− mutant were also proportional to the observed contents of galactokinase. In cultures repressed for the enzymes of the galactose operon with thiomethylgalactoside, the content of gal-mRNA was higher than expected from the content of galactokinase. Possible explanations of this finding are discussed.

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