scholarly journals Serial sodium depletion and NaCl solution intake

1966 ◽  
Vol 1 (1) ◽  
pp. 75-77 ◽  
Author(s):  
John L. Falk
2002 ◽  
Vol 282 (1) ◽  
pp. R235-R243 ◽  
Author(s):  
Lucia F. Franchini ◽  
Alan Kim Johnson ◽  
José de Olmos ◽  
Laura Vivas

We evaluated serotonergic hindbrain groups of cells for their involvement in the generation and inhibition of sodium appetite. For that purpose, we analyzed the number of Fos-immunoreactive (Fos-ir) cells and double-labeled Fos-serotonin (5-HT)-ir neurons within different nuclei of the hindbrain raphe system and the area postrema (AP). Sodium depletion and sodium appetite were induced by peritoneal dialysis. Twenty-four hours after peritoneal dialysis, a 2% NaCl solution intake test was given to peritoneal dialyzed animals [PD-with access (PD-A) group] and to control dialyzed animals [CD-with access (CD-A) group]. Two additional groups of animals received either peritoneal dialysis or control dialysis but were not given access to the 2% NaCl [CD-no access (CD-NA) group or PD-no access (PD-NA) group]. The number of Fos-ir neurons within different nuclei of the raphe system was increased in spontaneous and induced sodium ingestion of CD-A and PD-A groups compared with the CD-NA and PD-NA groups. The PD-NA group had significantly fewer double-labeled cells along the raphe system compared with the animals in near-normal sodium balance (CD-NA and CD-A) or in the process of restoring sodium balance by consuming NaCl (PD-A). The AP of the PD-A group showed a significant increase in the number of Fos-ir and Fos-5-HT-ir cells compared with the PD-NA and CD groups. Our results suggest that serotonergic pathways with cell bodies in the AP and the raphe system are involved in the control of sodium appetite.


1987 ◽  
Vol 28 (2) ◽  
pp. 209-211 ◽  
Author(s):  
Hisashi Kuribara ◽  
John L. Falk ◽  
Maisy Tang

2007 ◽  
Vol 92 (5) ◽  
pp. 781-789 ◽  
Author(s):  
Michael R. Bykowski ◽  
James C. Smith ◽  
Edward M. Stricker

1995 ◽  
Vol 57 (1) ◽  
pp. 113-116 ◽  
Author(s):  
Roser A. Nadal ◽  
Marc A. Pallares ◽  
Núria S. Ferre

1965 ◽  
Vol 16 (1) ◽  
pp. 315-325 ◽  
Author(s):  
John L. Falk

Rats were sodium depleted by intraperitoneal dialysis (IPD) to determine: (a) whether there is a thirst mechanism not dependent on increases in effective osmotic pressure and (b) whether a sodium chloride appetite could be induced by this rapid means of sodium depletion. IPD resulted in increased intakes of water and 3% NaCl solution when these were tested independently (single-stimulus method) A second IPD performed after 2 days of recovery produced even greater increases in intake. When water and 3% NaCl were available simultaneously after both dialyses, the increased salt intake was shown to be the predominant effect. The increased water intake occurred in spite of cellular overhydration and decreased serum Na level.


2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Michael R Bykowski ◽  
Myriam L Hoffmann ◽  
James C. Smith ◽  
Edward M. Stricker

1983 ◽  
Vol 18 (6) ◽  
pp. 983-984 ◽  
Author(s):  
M TANG ◽  
C BROWN ◽  
D MAIER ◽  
J FALK

Author(s):  
W. Liebrich

HeLa cells were grown for 2-3 days in EAGLE'S minimum essential medium with 10% calf serum (S-MEM; Seromed, München) and then incubated for 24 hours in serum free medium (MEM). After detaching the cells with a solution of 0. 14 % EDTA and 0. 07 % trypsin (Difco, 1 : 250) they were suspended in various solutions (S-MEM = control, MEM, buffered salt solutions with or without Me++ions, 0. 9 % NaCl solution) and allowed to settle on glass tube slips (Leighton-tubes). After 5, 10, 15, 20, 25, 30, 1 45, 60 minutes 2, 3, 4, 5 hours cells were prepared for scanning electron microscopy as described by Paweletz and Schroeter. The preparations were examined in a Jeol SEM (JSM-U3) at 25 KV without tilting.The suspended spherical HeLa cells are able to adhere to the glass support in all solutions. The rate of attachment, however, is faster in solutions without serum than in the control. The latter is in agreement with the findings of other authors.


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