Monoclonal antibodies demonstrate similarity of surface antigens on different clones of Schistosoma mansoni schistosomula

1984 ◽  
Vol 78 (2) ◽  
pp. 187-189 ◽  
Author(s):  
M.A. Smith ◽  
J.A. Clegg ◽  
D. Snary
Keyword(s):  
Monoclonal Antibodies ◽  
Schistosoma Mansoni ◽  
Surface Antigens

The identification of Schistosoma mansoni surface antigens recognized by protective monoclonal antibodies

1986 ◽  
Vol 8 (2) ◽  
pp. 193-198 ◽  
Author(s):  
C. KELLY ◽  
A. J. G. SIMPSON ◽  
E. FOX ◽  
S. M. PHILLIPS ◽  
S. R. SMITHERS
Keyword(s):  
Monoclonal Antibodies ◽  
Schistosoma Mansoni ◽  
Surface Antigens

scholarly journals Visual detection of granulocyte surface antigens using the avidin-biotin complex.

1984 ◽  
Vol 32 (7) ◽  
pp. 712-716 ◽  
Author(s):  
M Henke ◽  
L M Yonemoto ◽  
G S Lazar ◽  
L Gaidulis ◽  
T Hecht ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Indirect Immunofluorescence ◽  
Visual Detection ◽  
Hla Antigens ◽  
Surface Antigens ◽  
Cell Populations ◽  
Surface Markers ◽  
Visual Test ◽  
Avidin Biotin Complex

A visual test for detection of granulocyte surface markers using the avidin-biotin complex (ABC) has been developed. That this assay is highly specific, reproducible, and sensitive was determined by studying the expression of HLA antigens on granulocytes with monoclonal antibodies. Further, using granulocyte specific alloantisera, the results of the ABC test compared well to data from leukoagglutination assays and indirect immunofluorescence tests. The assay is particularly advantageous in that granulocytes can be stored, only small amounts of cells and sera are needed, and heterogeneous cell populations can easily be studied.

Monoclonal antibodies against surface antigens of schistosomula of Schistosoma mansoni

Parasitology ◽  
1982 ◽  
Vol 84 (1) ◽  
pp. 65-82 ◽  
Author(s):  
D. W. Taylor ◽  
A. F. Butterworth
Keyword(s):  
Monoclonal Antibodies ◽  
Gel Electrophoresis ◽  
Primary Infection ◽  
Polyacrylamide Gel Electrophoresis ◽  
Apparent Molecular Weight ◽  
Surface Antigens ◽  
Soft Agar ◽  
Surface Proteins ◽  
Spleen Cells

SUMMARYMonoclonal antibodies have been produced after fusion of NS-1 murine myeloma cells with spleen cells from mice immunized either by chronic primary infection or with irradiated cercariae: in both cases, animals were challenged with live cercariae 7 days before fusion. The initial cultures were screened for anti-schistosomular antibodies both by a radioimmunoassay with whole schistosomulum extracts and by immunofluorescence. There was no correlation between the two techniques and subsequent screening was carried out by immunofluorescence. Cloning was carried out in soft agar and 7 cloned cell lines, from 5 initial cultures, were selected for detailed study. Products of 6 of these 7 lines were monoclonal, as judged by isoelectricfocusing of [35S]methionine-labelled supernatant fluids, and their binding to live schistosomula was specific. None of the antibodies showed detectable activity in mediating eosinophil- or complement-dependent damage to schistosomula in vitro. However, 2 antibodies were successfully used to isolate surface proteins with an apparent molecular weight of 24000 on SDS-polyacrylamide gel electrophoresis.

Analysis of cell surface antigens on glucocorticoid-treated rat thymocytes with monoclonal antibodies

1994 ◽  
Vol 39 (3) ◽  
pp. 209-217 ◽  
Author(s):  
M. Tsuchida ◽  
M. Konishi ◽  
K. Jojima ◽  
K. Naito ◽  
Y. Fujikura ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Surface Antigens ◽  
Cell Surface Antigens

Protective antigens of bloodstage Plasmodium knowlesi parasites

1984 ◽  
Vol 307 (1131) ◽  
pp. 159-169 ◽  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Plasmodium Knowlesi ◽  
Surface Antigens ◽  
Red Cell ◽  
Cell Surface Antigens ◽  
Susceptible Host ◽  
Protective Antigens ◽  
Stage Of Development

The simian malaria Plasmodium knowlesi provides many favourable features as an experimental model; it can be grown in vivo or in vitro . Parasites of defined variant specificity and stage of development are readily obtained and both the natural host and a highly susceptible host are available for experimental infection and vaccination trials. Proteins synthesized by erythrocytic P. knowlesi parasites are characteristic of the developmental stage, as are the alterations that the parasite induces in the red cell surface. Erythrocytic merozoites are anatomically and biochemically complex, their surface alone is covered by at least eight distinct polypeptides. Immune serum from merozoite-immunized rhesus recognizes many parasite components, especially those synthesized by schizonts. All of the merozoite surface components and some of the schizont-infected red cell surface antigens are recognized by such immune sera. Rhesus monkeys rendered immune by repeated infection may by contrast recognize comparatively few antigens; a positive correlation was established for these ‘ naturally ’ immunized monkeys between protection and antibody directed against a 74000 molecular mass antigen. Im m unization with this purified antigen confers partial protection. O ther putative protective antigens have been identified by monoclonal antibodies that inhibit merozoite invasion of red cells in vitro . The antigens recognized by inhibitory monoclonal antibodies are synthesized exclusively by schizonts and are processed, at the time ofschizont rupture and merozoite release, to smaller molecules that are present on the merozoite surface. The multiplicity of protective antigens is clearly demonstrated by the fact that seven distinct merozoite surface antigens are recognized by three different inhibitory monoclonals. None of the protective antigens identified are variant or strain specific.

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