Schistosoma Mansoni: the Identification of Some Highly Immunogenic Surface Antigens of the Lung Stage Larvae as Promising Vaccine Candidates Against Schistosomiasis

ABSTRACT Nontypeable Haemophilus influenzae (NTHi) is a common cause of otitis media and respiratory tract infections. Outer membrane proteins (OMP) and lipooligosaccharide (LOS) are major surface antigens of NTHi and potential vaccine candidates. De-O-acylated LOS (dLOS) or oligosaccharide (OS) was coupled to total OMP to form dLOS-OMP and OS-OMP conjugates, while a dLOS-tetanus toxoid (TT) was synthesized for comparison. These conjugates were evaluated in mice and rabbits for immunogenicity. dLOS-OMP elicited a better boostable antibody response against LOS than did dLOS-TT, while OS-OMP was not immunogenic. Formulation of the conjugates with Ribi adjuvant significantly enhanced the immunogenicity of dLOS-OMP and dLOS-TT but not that of OS-OMP. In addition, rabbit antisera elicited by dLOS-OMP but not dLOS-TT (or OMP alone) demonstrated bactericidal activity against 40% of the NTHi strains tested. These results indicate that dLOS is a better derivative of LOS than OS and that OMP is a good carrier for NTHi LOS-based conjugate vaccines.

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Temporal differences in praziquantel- and oxamniquine-induced tegumental damage to adult Schistosoma mansoni: implications for drug-antibody synergy

Parasitology ◽

10.1017/s0031182000065069 ◽

1996 ◽

Vol 112 (1) ◽

pp. 47-58 ◽

Cited By ~ 33

Author(s):

P. G. Fallon ◽

R. E. Fookes ◽

G. A. Wharton

Keyword(s):

Drug Treatment ◽

Schistosoma Mansoni ◽

Adult Worm ◽

Surface Antigens ◽

Drug Induced ◽

Temporal Differences ◽

Temporal Study ◽

Praziquantel Treatment

SUMMARYA temporal study of the effects on the tegument of Schistosoma mansoni adult worm following in vivo praziquantel and oxamniquine treatment was performed. Drug-induced damage to the tegument, exposure of surface antigens and attachment of host antibody occurred rapidly, within 1 h, following praziquantel treatment. Oxamniquine-treated worms required 4–8 days for these effects to be apparent. The 2 drugs differed in the degree and sites of damage on the worm surface. The administration of 2 different polyspecific rabbit sera with drug significantly increased the efficacy of praziquantel when administered with the drug, but not when given 6–9 days after drug treatment. In contrast, only 1 serum was synergistic with oxamniquine when administered with drug and both sera were synergistic when given 6–9 days after drug treatment. The effect of immune killing of drug-treated worms is discussed.

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Isolation and characterization of surface antigens from Schistosoma mansoni schistosomula

Molecular and Biochemical Parasitology ◽

10.1016/0166-6851(81)90053-0 ◽

1981 ◽

Vol 3 (4) ◽

pp. 215-225 ◽

Cited By ~ 60

Author(s):

Colette Dissous ◽

Claude Dissous ◽

André Capron

Keyword(s):

Schistosoma Mansoni ◽

Surface Antigens ◽

Isolation And Characterization

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