Negative ion ammonia chemical ionization and electron impact ionization mass spectrometric analysis of steryl fatty acyl esters

Steroids ◽  
1989 ◽  
Vol 53 (3-5) ◽  
pp. 285-309 ◽  
Author(s):  
Richard P. Evershed ◽  
Mark C. Prescott ◽  
Neil Spooner ◽  
L.John Goad
Keyword(s):  
Electron Impact ◽  
Chemical Ionization ◽  
Impact Ionization ◽  
Electron Impact Ionization ◽  
Fatty Acyl ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Negative Ion ◽  
Spectrometric Analysis ◽  
Ionization Mass

Gas-Liquid Chromatographic/Mass Spectrometric Screening Method for T-2 Toxin in Milk

1979 ◽  
Vol 62 (6) ◽  
pp. 1274-1280
Author(s):  
George J Collins ◽  
Joseph D Rosen
Keyword(s):  
Mass Spectrometry ◽  
Electron Impact ◽  
Chemical Ionization ◽  
Impact Ionization ◽  
Trimethylsilyl Ether ◽  
Screening Method ◽  
Internal Standard ◽  
Electron Impact Ionization ◽  
Gas Liquid Chromatography ◽  
Ionization Mass

Abstract A method for the analysis of T-2 toxin in milk is presented. Ethyl acetate extracts of milk samples which had been spiked with T-2 toxin were purified by thin layer chromatography and derivatized with N,O-bis(trimethylsilyl)acetamide to produce the T-2 toxin trimethylsilyl ether (T-2 toxin-TMS). N,O-bis(trimethylsilyl-d9)acetamide was used to make T-2 toxin d9-trimethylsilyl ether (T-2 toxin-d9TMS) which was added to the derivatized milk extract as an internal standard. Samples were analyzed by combined gas-liquid chromatography/mass spectrometry using either electron impact ionization or chemical ionization mass spectrometry. In electron impact ionization analyses, simultaneous monitoring of the T-2 toxin-TMS fragment ion at m/z 436 and the T-2 toxin-d9TMS fragment ion at m/z 445 gave a T-2 toxin-TMS detectability estimated at 6 μg/kg. In chemical ionization analyses, the T-2 toxin-TMS fragment ion at m/z 377 and the T-2 toxin-d9TMS fragment ion at m/z 386 were simultaneously monitored to give a T-2 toxin-TMS detectability estimated at 3 μg/kg. Average recovery was 85% at 200 μg/kg and 65% at 20 μg/kg.

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