Visualization of the outer and interblastomeric surface of early embryos of Xenopus laevis by scanning electron microscopy

Ceratophrys ornata, the Argentinean horned frog, has a significantly different pattern of early morphogenesis than does the most studied amphibian, Xenopus laevis. Time-lapse videomicroscopy, scanning electron microscopy, histological sections and lineage tracers have shown that, in C. ornata, some prospective notochord, somite and tailbud mesoderm cells leave the surface epithelium of the archenteron by ingression. After gastrulation, SEM reveals cells with constricted apices and a bottle shape in three zones on the archenteron roof and in a fourth zone around the blastopore. Prospective somitic tissue ingresses first from two lateral zones, followed by ingression of prospective notochord from the medial zone and tailbud mesoderm from the circumblastoporal zone. This is unlike X. laevis, in which no cells with constricted apices are present on the dorsal surface of the archenteron, nor do any cells ingress into the deep mesodermal layers from the surface layer.

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Microvascular Anatomy of Extrahepatic Bile Ducts in Adult South African Clawed Toad, Xenopus laevis Daudin: Scanning Electron Microscopy of Vascular Corrosion Casts and Correlative Light Microscopy

Microscopy and Microanalysis ◽

10.1017/s1431927609093970 ◽

2009 ◽

Vol 15 (S2) ◽

pp. 986-987

Author(s):

A Lametschwandtner ◽

U Lametschwandtner ◽

H Bartel ◽

C Radner ◽

B Minnich

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Xenopus Laevis ◽

Light Microscopy ◽

South African ◽

Bile Ducts ◽

Corrosion Casts ◽

Extrahepatic Bile Ducts ◽

Scanning Electron ◽

Vascular Corrosion Casts

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

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Microvascularization of the interhyoid muscle in larval Xenopus laevis (Daudin): Scanning electron microscopy of vascular corrosion casts and correlative light microscopy

Journal of Morphology ◽

10.1002/jmor.10918 ◽

2010 ◽

Vol 272 (3) ◽

pp. 342-353 ◽

Cited By ~ 1

Author(s):

J. Rattey ◽

A. Lametschwandtner

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Xenopus Laevis ◽

Light Microscopy ◽

Corrosion Casts ◽

Scanning Electron ◽

Vascular Corrosion Casts

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Scanning electron microscopy of cells isolated from amphibian early embryos

Development ◽

10.1242/dev.48.1.215 ◽

1978 ◽

Vol 48 (1) ◽

pp. 215-223

Author(s):

Martin Stanisstreet ◽

James L. Smith

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Early Embryos ◽

Scanning Electron

Cells have been dissociated from Xenopus and Ambystoma late blastulae, allowed to adhere to glass coverslips, and studied by scanning electron microscopy. Xenopus ectoderm cells initially show filopodia; later larger single pseudopodia are formed. Ambystoma ectoderm cells show fewer filopodia than Xenopus ectoderm, but later form pseudopodia. Ectoderm cells of both Xenopus and Ambystoma show links between adjacent cells. Xenopus endoderm cells do not show filopodia initially, but later show large pseudopodia.

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