Endothelium-derived relaxing factor from cultured human endothelial cells inhibits aggregation of human platelets

1987 ◽  
Vol 47 (5) ◽  
pp. 561-571 ◽  
Author(s):  
Ute Alheid ◽  
Jürgen C. Frölich ◽  
Ulrich Förstermann
Keyword(s):  
Endothelial Cells ◽  
Human Platelets ◽  
Human Endothelial Cells ◽  
Relaxing Factor ◽  
Endothelium Derived Relaxing Factor

scholarly journals Inhibition by l-glutamine of the release of endothelium-derived relaxing factor from cultured endothelial cells

1990 ◽  
Vol 101 (2) ◽  
pp. 237-239 ◽  
Author(s):  
Markus Hecker ◽  
Jane A. Mitchell ◽  
Tomasz A. Swierkosz ◽  
William C. Sessa ◽  
John R. Vane
Keyword(s):  
Endothelial Cells ◽  
Relaxing Factor ◽  
Cultured Endothelial Cells ◽  
Endothelium Derived Relaxing Factor

Canine arteries release two different endothelium-derived relaxing factors

1989 ◽  
Vol 257 (1) ◽  
pp. H330-H333 ◽  
Author(s):  
U. Hoeffner ◽  
M. Feletou ◽  
N. A. Flavahan ◽  
P. M. Vanhoutte
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Coronary Artery ◽  
Vascular Smooth Muscle ◽  
Coronary Arteries ◽  
Relaxing Factor ◽  
Prostaglandin F2 Alpha ◽  
Donor Artery ◽  
Canine Coronary Artery ◽  
Endothelium Derived Relaxing Factor

Experiments were designed to analyze the effects of ouabain on the response of vascular smooth muscle to endothelium-derived relaxing factors released under basal conditions and on stimulation with acetylcholine or bradykinin. Bioassay rings of canine coronary artery (without endothelium) were superfused with perfusate from canine left circumflex coronary arteries with endothelium (donor arteries). During contractions of the bioassay ring evoked by prostaglandin F2 alpha, the relaxations caused by endothelium-derived relaxing factor(s), released under basal conditions or on exposure of the endothelial cells of the donor artery to maximally effective concentrations of acetylcholine, were reduced by incubation of the bioassay ring with ouabain. However, the relaxations evoked by infusion of bradykinin were not altered by incubation of the bioassay rings with ouabain. These experiments demonstrate the release of two endothelium-derived relaxing factors that can be distinguished using ouabain.

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