The cross-reactivity of IgE antibodies with pollen allergens

The cross-reactivity of antibodies employed within immunochemistry-based analytical methods may lead to overestimation of the results. Under certain conditions, specifically when controlling mycotoxin maximum limits serious problems can be encountered. Not only the structurally related mycotoxins, such as their masked (conjugated) forms, but also the unidentified matrix components are responsible for concentration overestimation of respective target analytes. The cross-reactivity phenomenon may also pose a risk of miss-interpretation of the proficiency tests results, when the assigned value becomes influenced by over-estimated results reported by users of immunochemical tests. In this paper, the current state of the knowledge on trueness problems associated with the rapid screening immunochemical methods have been reviewed. Special attention is focused on discussion of cross-reactivity in the ELISA tests, because this rapid test dominates the routine screening practice. However, the cross-reactions reported in lateral flow test strips, fluorescence polarisation immunoassay, or immunosensors have also been addressed.

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Immunity to tapeworms: intraspecific cross-protective interactions betweenHymenolepis citelli, H. diminutaandH. microstomain mice

Parasitology ◽

10.1017/s0031182000065525 ◽

1986 ◽

Vol 92 (3) ◽

pp. 665-674 ◽

Cited By ~ 6

Author(s):

Sidi T. O. Alghali ◽

R. K. Grencis

Keyword(s):

Primary Infection ◽

Cross Reactivity ◽

Significant Protection ◽

Cyst Infection ◽

Single Host ◽

Concurrent Infections ◽

Rejection Mechanism ◽

The Cross

SUMMARYInteractions between tapeworm species in a single host offer intriguing opportunities for immunological studies that attempt to identify the mechanism(s) underlying protection against cestode infections. Mice that are immunized againstHymenolepis citelliinfections were shown to be refractory to subsequentH. diminutachallenge infections. The reciprocity of the response was also demonstrated, although the protection recorded forH. diminutawhen mice are sensitized withH. citelliis weaker than that observed when mice are primed withH. diminutaagainstH. citellichallenge.H. citelliwas also shown to be expelled simultaneously during the rejection phase ofH. diminutain concurrent infections, indicating the susceptibility of the former tapeworm to the rejection mechanism initiated by the latter.H. microstomaimmunized mice were shown to be strongly protected against heterologousH. citellichallenge. However, mice primed againstH. citelliwere not as strongly protected againstH. microstomachallenge infections: a statistically significant protection was obtained only after a 12-cysticercoidH. citelliprimary infection, although a 6-cyst infection did stunt the growth ofH. microstomachallenge worms. It is presently suggested that the cross-protective responses observed in the study betweenH. citelli, H. diminutaandH. microstomamay have emanated from a specific immunological cross-reactivity due to the sharing of similar immunogens.

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Ceftaroline Desensitization Procedure in a Pregnant Patient With Multiple Drug Allergies

Open Forum Infectious Diseases ◽

10.1093/ofid/ofv027 ◽

2015 ◽

Vol 2 (1) ◽

Cited By ~ 3

Author(s):

James L. Kuhlen ◽

Kimberly G. Blumenthal ◽

Caroline L. Sokol ◽

Diana S. Balekian ◽

Ana A. Weil ◽

...

Keyword(s):

Cystic Fibrosis ◽

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Skin Testing ◽

Pregnant Patient ◽

Cross Reactivity ◽

Multiple Drug ◽

Methicillin Resistant ◽

The Cross ◽

Staphylococcus Aureus Pneumonia

Abstract Validated skin testing is lacking for many drugs, including ceftaroline. The cross-reactivity between ceftaroline and other β-lactam antibiotics is unknown. We report a case of a pregnant patient with cystic fibrosis and multiple drug allergies who required ceftaroline for methicillin-resistant Staphylococcus aureus pneumonia and underwent an uncomplicated empiric desensitization procedure.

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Allergy to cephalosporin antibiotics in children

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh0304127a ◽

2003 ◽

Vol 131 (3-4) ◽

pp. 127-130 ◽

Cited By ~ 1

Author(s):

Marina Atanaskovic-Markovic ◽

Branimir Nestorovic

Keyword(s):

Allergic Reaction ◽

Cross Reactivity ◽

Skin Tests ◽

Allergic Reactions ◽

First Line ◽

Positive Skin ◽

Cephalosporin Antibiotics ◽

The Cross ◽

Single Blind ◽

Common Infections

A particular problem is the safety of administering cephalosporins to penicillin-allergic children, because cephalosporin allergenic determinants have not been properly identified. Cephalosporin antibiotics are widely used to treat common infections and are often the first-line prophylaxis before many types of surgery. So the arm of this study is to determine the frequency of allergic reactions of anaphylactic type to cephalosporins and their cross-reactivity with penicillins. At University Children?s Hospital in Belgrade a group of 1,170 children with suspected anaphylactic allergic reaction to penicillins and/or cephalosporins were tested for the last eight years. Skin tests were performed with standard concentration of penicillins and cephalosporins. In children where skin tests were negative single-blind placebo-controlled challenges were performed. In case of positive skin tests further examinations were interrupted and the children were considered allergic to that drug. The frequency of anaphylactic allergic reactions to cephalosporins is 0.2 % to 17 %, and depends on cephalosporins generation. The cross-reactivity between cephalosporins and penicillins is 0.1 % to 14.5 %, and among cephalosporins is 0 % to 11.7 %.

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Cross-Reactivity between IgE-Binding Proteins from Anisakis Simplex and Dermatophagoides Pteronyssinus

International Journal of Immunopathology and Pharmacology ◽

10.1177/039463200501800408 ◽

2005 ◽

Vol 18 (4) ◽

pp. 671-675 ◽

Cited By ~ 25

Author(s):

R. Bernardini ◽

G. Mistrello ◽

E. Novembre ◽

D. Roncarolo ◽

S. Zanotta ◽

...

Keyword(s):

Molecular Weight ◽

Binding Proteins ◽

Specific Ige ◽

Dermatophagoides Pteronyssinus ◽

Cross Reactivity ◽

Anisakis Simplex ◽

Cross Reaction ◽

Ige Binding ◽

Sds Page ◽

The Cross

An association was found between Anisakis simplex (As) and Dermatophagoides pteronyssinus (Dp) sensitization. One recent study shows a cross-reactivity between As and Dp and tropomyosin (tr) is suspected as being one of the proteins responsible of this cross-reaction. The aim of our study was: 1) to confirm the cross-reactivity between Dp and As; 2) to determine the importance of tr in this cross reaction. SDS-PAGE analysis of Dp and As (metabolic and somatic) extracts was carried out. Then an IgE immunoblotting test using serum from a patient who had specific IgE only to Dp and As and immunoblotting inhibition experiments using Dp extract and tr as inhibitors were performed. We found that patient's serum reacted: 1) against larval As antigens with a molecular weight (mw) of 25 kilodalton (kD) and a mw > 100 kD, 2) against various metabolic As antigens with a mw > 100 kD, a mw ranging approximately from 35 to 50 kD, and a mw around 20 kD, and 3) against Dp proteins with mw between 35 and 55 kD. Preincubation of patient's serum with Dp extract caused the disappearance of reactivity against antigens with a mw > 100 kD in both larval and metabolic As extracts and against proteins with mw ranging approximately from 35 to 50 kD in the metabolic As extract. Preincubation of patient's serum with As extract caused the disappearance of reactivity against antigens with mw between 35 and 55 kD in the Dp extract. Pre-incubation of patient's serum with tr did not induce any change in the immunoblotting profile. The results show that 1) cross-reactive components between Dp and As are some proteins with a mw ranging approximately from 35 to 50 kD and with a mw > 100 kD, and 2) tr is not involved in cross-reactivity between As and Dp.

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