In vitro splicing of the ribosomal RNA precursor of tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequence

Cell ◽  
1981 ◽  
Vol 27 (3) ◽  
pp. 487-496 ◽  
Author(s):  
Thomas R. Cech ◽  
Arthur J. Zaug ◽  
Paula J. Grabowski
Keyword(s):  
Ribosomal Rna ◽  
In Vitro Splicing ◽  
Rna Precursor

scholarly journals In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena

1980 ◽  
Vol 8 (23) ◽  
pp. 5551-5566 ◽  
Author(s):  
M. Carin ◽  
B.F. Jensen ◽  
K.D. Jentsch ◽  
J.C. Leer ◽  
O.F. Nielsen ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
In Vitro Splicing ◽  
Rna Precursor

In vitro splicing of the ribosomal RNA precursor in nuclei of tetrahymena

Cell ◽  
1980 ◽  
Vol 19 (2) ◽  
pp. 331-338 ◽  
Author(s):  
Arthur J. Zaug ◽  
Thomas R. Cech
Keyword(s):  
Ribosomal Rna ◽  
In Vitro Splicing ◽  
Rna Precursor

Synthesis of RNA in oocytes of Xenopus laevis during culture in vitro

Development ◽  
1974 ◽  
Vol 32 (2) ◽  
pp. 515-532
Author(s):  
A. Colman
Keyword(s):  
Xenopus Laevis ◽  
In Vitro Culture ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Salt Medium ◽  
Complex Media ◽  
18S Ribosomal Rna ◽  
Rna Precursor

RNA synthesis can be maintained in large oocytes of Xenopus laevis during periods of in vitro culture of at least 10 days. A simple salt medium, modified Barth's solution, is found to be as effective a culture medium for these oocytes as several other complex media. The newly synthesized RNA is characterized electrophoretically and shown to consist predominantly of ribosomal RNA precursor, 28S and 18S ribosomal RNA, and 4S RNA. The distribution of this RNA within the oocyte is detected autoradiographically, where it is found to be greatly concentrated over the nucleoli. No qualitative alterations in either of these parameters are found during culture, within the limits of sensitivity of the assay procedures.

scholarly journals Processing of Naked 45-S Ribosomal RNA Precursor in vitro by an RNA-Associated Endoribonuclease

1981 ◽  
Vol 115 (2) ◽  
pp. 375-383 ◽  
Author(s):  
Claudio DENOYA ◽  
Patricia COSTA GIOMI ◽  
Eduardo A. SCODELLER ◽  
Cesar VASQUEZ ◽  
Jose L. TORRE
Keyword(s):  
Ribosomal Rna ◽  
Rna Precursor

Design and synthesis of herboxidiene derivatives that potently inhibit in vitro splicing

2021 ◽  
Vol 19 (6) ◽  
pp. 1365-1377
Author(s):  
Arun K. Ghosh ◽  
Srinivasa Rao Allu ◽  
Guddeti Chandrashekar Reddy ◽  
Adriana Gamboa Lopez ◽  
Patricia Mendez ◽  
...  
Keyword(s):  
Biological Evaluation ◽  
Design And Synthesis ◽  
In Vitro Splicing ◽  
Enantioselective Syntheses ◽  
Derivatives Of

Enantioselective syntheses of C-6 modified derivatives of herboxidiene and their biological evaluation in splicing inhibitory assay.

scholarly journals DIFFERENTIATION OF DROSOPHILA CELLS LACKING RIBOSOMAL DNA, IN VITRO

Genetics ◽  
1973 ◽  
Vol 73 (3) ◽  
pp. 429-434
Author(s):  
J James Donady ◽  
R L Seecof ◽  
M A Fox
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Ribosomal Dna ◽  
Rna Synthesis ◽  
Wild Type ◽  
Drosophila Cells

ABSTRACT Drosophila melanogaster embryos that lacked ribosomal DNA were obtained from appropriate crosses. Cells were taken from such embryos before overt differentiation took place and were cultured in vitro. These cells differentiated into neurons and myocytes with the same success as did wild-type controls. Therefore, ribosomal RNA synthesis is not necessary for the differentiation of neurons and myocytes in vitro.

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