Single channel recordings from calcium-activated potassium channels in cultured rat muscle

Cell Calcium ◽  
1983 ◽  
Vol 4 (5-6) ◽  
pp. 359-370 ◽  
Author(s):  
Barry S. Pallotta
Keyword(s):  
Potassium Channels ◽  
Single Channel ◽  
Single Channel Recordings ◽  
Rat Muscle ◽  
Calcium Activated Potassium Channels

scholarly journals How Does the W434F Mutation Block Current in Shaker Potassium Channels?

1997 ◽  
Vol 109 (6) ◽  
pp. 779-789 ◽  
Author(s):  
Youshan Yang ◽  
Yangyang Yan ◽  
Fred J. Sigworth
Keyword(s):  
Potassium Channels ◽  
Potassium Current ◽  
Xenopus Oocytes ◽  
Single Channel ◽  
Ion Selectivity ◽  
Independent Component ◽  
Single Channel Recordings ◽  
Shaker Channels ◽  
Rapid Inactivation ◽  
Shaker Potassium Channels

The mutation W434F produces an apparently complete block of potassium current in Shaker channels expressed in Xenopus oocytes. Tandem tetrameric constructs containing one or two subunits with this mutation showed rapid inactivation, although the NH2-terminal inactivation domain was absent from these constructs. The inactivation showed a selective dependence on external cations and was slowed by external TEA; these properties are characteristic of C-type inactivation. Inactivation was, however, incompletely relieved by hyperpolarization, suggesting the presence of a voltage-independent component. The hybrid channels had near-normal conductance and ion selectivity. Single-channel recordings from patches containing many W434F channels showed occasional channel openings, consistent with open probabilities of 10−5 or less. We conclude that the W434F mutation produces a channel that is predominantly found in an inactivated state.

Single channel recordings of Ca2+-activated K+ currents in rat muscle cell culture

Nature ◽  
1981 ◽  
Vol 293 (5832) ◽  
pp. 471-474 ◽  
Author(s):  
Barry S. Pallotta ◽  
Karl L. Magleby ◽  
John N. Barrett
Keyword(s):  
Cell Culture ◽  
Muscle Cell ◽  
Single Channel ◽  
Muscle Cell Culture ◽  
Single Channel Recordings ◽  
Rat Muscle ◽  
K Currents
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