Effects of neuropeptide-Y and substance-P on the secretory activity of dispersed zona-glomerulosa cells of rat adrenal gland

Neuropeptides ◽  
1990 ◽  
Vol 17 (3) ◽  
pp. 121-125 ◽  
Author(s):  
G. Neri ◽  
P.G. Andries ◽  
G.G. Nussdorfer
Keyword(s):  
Substance P ◽  
Adrenal Gland ◽  
Neuropeptide Y ◽  
Secretory Activity ◽  
Zona Glomerulosa ◽  
Glomerulosa Cells ◽  
Zona Glomerulosa Cells

Effects of sodium depletion on the response of rat adrenal zona glomerulosa cells to stimulation by neuropeptides: actions of vasoactive intestinal peptide, enkephalin, substance P, neuropeptide Y and corticotrophin-releasing hormone

1995 ◽  
Vol 146 (2) ◽  
pp. 209-214 ◽  
Author(s):  
J P Hinson ◽  
S Kapas
Keyword(s):  
Angiotensin Ii ◽  
Substance P ◽  
Neuropeptide Y ◽  
Vasoactive Intestinal Peptide ◽  
Zona Glomerulosa ◽  
Angiotensin Ii Receptors ◽  
Sodium Depletion ◽  
Aldosterone Secretion ◽  
Glomerulosa Cells ◽  
Zona Glomerulosa Cells

Abstract There are several neuropeptides, present in nerves supplying the rat adrenal zona glomerulosa, which have been shown to stimulate aldosterone secretion in the intact perfused rat adrenal preparation. The purpose of the present study was twofold: first, to determine whether these peptides acted directly on adrenocortical cells by examining their effects on collagenase-dispersed rat zona glomerulosa cells, and second, to investigate the likely physiological significance of these actions, by determining whether the responses of zona glomerulosa cells to neuropeptides were changed by prior sodium depletion. Of the peptides tested, neuropeptide Y (NPY) and substance P had only a minor effect on aldosterone secretion, which was not substantially affected by sodium depletion. Corticotrophin-releasing hormone (CRH) had a significant stimulatory effect on aldosterone secretion, but neither the threshold concentration for significant stimulation nor the maximal response to stimulation were altered by prior sodium depletion. Vasoactive intestinal peptide (VIP), on the other hand, had little effect on aldosterone secretion by cells from normal animals, but was a potent stimulus to aldosterone secretion in cells obtained from sodium-depleted animals. The response to the Met-enkephalin analogue, [d-Ala2-Met2]-enkephalinamide (DALA), was also significantly enhanced by prior sodium depletion. Experiments using the angiotensin II receptor blocker, saralasin, were carried out to determine whether the enhanced actions of DALA and VIP seen in sodium depletion may be a result of activation of angiotensin II receptors, known to be increased in sodium depletion. Saralasin did not affect the response to either peptide. These data suggest that all the peptides tested may be able to stimulate aldosterone secretion. However, the data obtained with substance P, NPY and CRH do not support a major role for these peptides in the regulation of aldosterone secretion either under control conditions, or in sodium depletion. The finding that the responses to VIP and DALA were altered by sodium depletion suggests that the actions of VIP and opioid peptides may have physiological significance in the regulation of aldosterone secretion in response to sodium depletion. Furthermore, the observation that saralasin does not inhibit the responses to these peptides strongly suggests that they are not acting through angiotensin II receptors, and may indicate altered VIP- and opioid-receptor regulation in sodium depletion. Journal of Endocrinology (1995) 146, 209–214

Endothelin-1 acutely stimulates the secretory activity of rat zona glomerulosa cells

Peptides ◽  
1990 ◽  
Vol 11 (4) ◽  
pp. 763-765 ◽  
Author(s):  
Giuseppina Mazzocchi ◽  
Ludwik K. Malendowicz ◽  
Gastone G. Nussdorfer
Keyword(s):  
Secretory Activity ◽  
Zona Glomerulosa ◽  
Endothelin 1 ◽  
Glomerulosa Cells ◽  
Zona Glomerulosa Cells

Neuropeptide Y modulates the sensitivity of the rat adrenal cortex to stimulation by ACTH

1995 ◽  
Vol 145 (2) ◽  
pp. 283-289 ◽  
Author(s):  
J P Hinson ◽  
L A Cameron ◽  
S Kapas
Keyword(s):  
Angiotensin Ii ◽  
Neuropeptide Y ◽  
Adrenal Cortex ◽  
Mammalian Species ◽  
Zona Glomerulosa ◽  
Zona Fasciculata ◽  
Steroid Secretion ◽  
Glomerulosa Cells ◽  
Zona Glomerulosa Cells

Abstract Neuropeptide Y (NPY) has been identified in nerves supplying the adrenal cortex of several mammalian species, although its function in this tissue is unknown. The present studies, employing adrenocortical cells prepared by collagenase digestion, have shown that NPY, in the absence of other stimulants, has no effect on steroid secretion by the rat adrenal over a range of peptide concentrations (10−11 to 10 −6 mol/l). However, in the presence of physiological concentrations of ACTH, which are submaximal for the stimulation of aldosterone secretion, NPY (10−6 mol/l) significantly enhanced the secretion rate of aldosterone by rat zona glomerulosa cells in response to ACTH. This effect was specific to the rat zona glomerulosa as NPY had no effect on the response to ACTH in rat zona fasciculata cells. The effect of NPY appears to be biphasic, however, as NPY significantly attenuated the steroidogenic response to supramaximal ACTH concentrations: in rat zona glomerulosa cells the aldosterone response to 10 −8 mol ACTH/l was significantly inhibited by NPY. The effect of NPY on the ACTH response appeared to be mediated by changes in the cAMP response. NPY had no effect on the steroidogenic response to potassium ions (K+), but enhanced the response to angiotensin II. NPY (10 −6 mol/l) significantly stimulated inositol 1,4,5-trisphosphate (InsP3) production although this concentration of peptide had no effect on steroid secretion. The effects of NPY on InsP3 production were additive with those of angiotensin II. These results suggest that the role of NPY in the adrenal cortex may be to regulate the sensitivity of the zona glomerulosa to peptide stimulation. Journal of Endocrinology (1995) 145, 283–289

scholarly journals EFFECTS OF BRADYKININ ON THE SECRETORY ACTIVITY OF DISPERSED ZONA GLOMERULOSA CELLS OF THE RAT ADRENAL CORTEX

1995 ◽  
Vol 16 (4) ◽  
pp. 251-254 ◽  
Author(s):  
LUDWIK K. MALENDOWICZ ◽  
PAOLA G. ANDREIS ◽  
GASTONE G. NUSSDORFER ◽  
JERZY B. WARCHOL ◽  
ANNA MARKOWSKA
Keyword(s):  
Secretory Activity ◽  
Zona Glomerulosa ◽  
Glomerulosa Cells ◽  
Zona Glomerulosa Cells

Electron Probe Microanalysis Of Spironolactone Bodies

1999 ◽  
Vol 5 (S2) ◽  
pp. 1288-1289
Author(s):  
J.P. McNeil ◽  
J.E. Carter ◽  
C.W. Boudreaux ◽  
F. McDonald ◽  
J.A. Tucker ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Adrenal Gland ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Zona Glomerulosa ◽  
Sem Analysis ◽  
Transmission Electron ◽  
Excess Production ◽  
Glomerulosa Cells ◽  
Zona Glomerulosa Cells

Spironolactone bodies (SB) were first described in 1963 by Janigan. These laminated, whorled structures are seen in cells of the adrenal zona glomerulosa in patients treated with the drug spironolactone. Spironolactone is an aldosterone antagonist. Hyperaldosteronism may result from excess production by the adrenal cortex. By both light microscopy and transmission electron microscopy (TEM), SB have a distinctive, laminated appearance. Kovacs, et al. observed that SB are composed of cellular constituents. To our knowledge, SB have not been analyzed using scanning electron microscopy (SEM) and electron probe microanalysis technology.An adrenal gland with a 1 cm cortical mass was removed from a 39 year old female and received in 10% buffered formalin. Histologic examination of the mass showed a monotonous population of cells recapitulating zona glomerulosa cells. Intracytoplasmic structures compatible with SB were identified. Portions of the adrenal gland were processed for TEM and SEM analysis.

scholarly journals Endothelium-Derived Steroidogenic Factor Enhances Angiotensin II-Stimulated Aldosterone Release by Bovine Zona Glomerulosa Cells

Endocrinology ◽  
2007 ◽  
Vol 148 (1) ◽  
pp. 317-323 ◽  
Author(s):  
Craig J. Hanke ◽  
Blythe B. Holmes ◽  
Yafei Xu ◽  
Kasem Nithipatikom ◽  
William B. Campbell
Keyword(s):  
Mass Spectrometry ◽  
Endothelial Cells ◽  
Angiotensin Ii ◽  
Adrenal Gland ◽  
Synergistic Effect ◽  
Zona Glomerulosa ◽  
Angiotensin Iv ◽  
Anatomical Relationship ◽  
Glomerulosa Cells ◽  
Zona Glomerulosa Cells

Endothelium-derived steroidogenic factor (EDSF) is an endothelial peptide that stimulates aldosterone release from bovine adrenal zona glomerulosa (ZG) cells. The regulation of aldosterone release by combinations of EDSF and angiotensin II (AII) or EDSF and ACTH was investigated. Endothelial cells (ECs) and EC-conditioned media (ECCM) increased aldosterone release from ZG cells, an activity attributed to EDSF. AII (10−12 to 10−8m) and ACTH (10−12 to 10−9m) also stimulated the release of aldosterone from ZG cells. The stimulation by AII, but not ACTH, was greatly enhanced when ZG cells were coincubated with ECs. AII was metabolized by ECs to peptides identified by mass spectrometry as angiotensin (1-7) and angiotensin IV. There was very little metabolism of AII by ZG cells. Neither of these two AII metabolites altered aldosterone release from ZG cells, so they could not account for the enhanced response with ECs. AII-induced aldosterone release from ZG cells was enhanced by ECCM but not cell-free conditioned medium. This enhanced response was not due to increased EDSF release from ECs by AII. The synergistic effect of EDSF and AII was apparent when AII was added during or after the generation of ECCM and not observed when the AII component of the enhancement was blocked by the AII antagonist, losartan. These studies indicate that EDSF enhances the steroidogenic effect of AII. In the adrenal gland, ECs are in close anatomical relationship with ZG cells and may sensitize ZG cells to the steroidogenic action of AII by releasing EDSF.

scholarly journals Histological characteristics of accessory adrenal glands of rabbits with different types of autonomous tonus

2019 ◽  
Vol 21 (93) ◽  
pp. 125-130 ◽  
Author(s):  
M. V. Zakrevska ◽  
A. M. Tybinka
Keyword(s):  
Adrenal Gland ◽  
Adrenal Glands ◽  
Zona Glomerulosa ◽  
Zona Fasciculata ◽  
White Breed ◽  
Histological Characteristics ◽  
Glomerulosa Cells ◽  
Morphological Research ◽  
Zona Glomerulosa Cells ◽  
Historical Indicators

For the purposes of the adrenal gland’s study, 27 four months old male rabbits (Oryctolagus cuniculus) of Termond White breed were selected. Based on electrocardiography and variation pulsometry results, three types of autonomous tonus were determined in animals: sympathicotonia (ST), normotonia (NT) and parasympathicotonia (PS), which formed the basis for the division of animals into three groups. All rabbits were subjected to euthanasia and histological preparations were made from their adrenal glands. Apart from the main adrenal gland, accessory adrenal gland was detected in five purposes animals, including three ST rabbits, one NT rabbit and one PS rabbit. Also, four accessory adrenal glands were revealed in one ST rabbit simultaneously. In fact, these accessory adrenal glands became the main object of the further morphological research. The obtained historical indicators accessory adrenal glands were compared to the ones from various animal groups (CT with NT and CT with PS), as well as to the indicators of the main adrenal gland in each group. Statistical analysis of the received data was performed only in the group of ST animals. Accessory adrenal gland of ST rabbits is represented by two zones: zona glomerulosa and zona fasciculata, the area of which is 52% and 48% respectively. In PS and NT rabbits, the glands are formed by zona glomerulosa only. Investigating the accessory adrenal gland’s cellular component, it was found that NT rabbits have the largest cell area in zona glomerulosa, while PS rabbits have the smallest one. At the same time, the area of nucleus has the largest values in ST animals and the smallest ones in PS animals likewise. Сomparing zona glomerulosa and zona fasciculata in ST rabbits, it was found that cell size differs significantly, while the area of nucleus is almost identical. The nuclear-cytoplasmic ratio in zona glomerulosa cells is the largest for PS rabbits. The smallest ratio values were detected among the NT rabbits. Obviously, ST rabbits occupy an intermediate position. Unlike the main adrenal gland, the cells of accessory adrenal gland are of a smaller size and а denser location of cells in all groups of animals. The conducted studies allow concluding that the typological features of the autonomous tonus affect the morphology of accessory adrenal gland.

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