Organotypic slice culture of the suprachiasmatic nucleus

1994 ◽  
Vol 18 (4) ◽  
pp. 601-604 ◽  
Author(s):  
Keiko Tominaga ◽  
Hitoshi Okamura ◽  
Shin-Ichi T. Inouye
Keyword(s):  
Suprachiasmatic Nucleus ◽  
Slice Culture ◽  
Organotypic Slice Culture

Survival and death of mature avian motoneurons in organotypic slice culture: Trophic requirements for survival and different types of degeneration

2007 ◽  
Vol 501 (5) ◽  
pp. 669-690 ◽  
Author(s):  
Núria Brunet ◽  
Olga Tarabal ◽  
Manel Portero-Otín ◽  
Ronald W. Oppenheim ◽  
Josep E. Esquerda ◽  
...  
Keyword(s):  
Slice Culture ◽  
Organotypic Slice Culture ◽  
Different Types

Synaptic Organization of an organotypic slice culture of the mammalian retina

1996 ◽  
Vol 13 (4) ◽  
pp. 759-771 ◽  
Author(s):  
Marco Sassoè-Pognetto ◽  
Andreas Feigenspan ◽  
Joachim Bormann ◽  
Heinz Wässle
Keyword(s):  
Hot Spots ◽  
Plexiform Layer ◽  
Slice Culture ◽  
Inner Plexiform Layer ◽  
Synaptic Organization ◽  
Glycine Receptors ◽  
Ribbon Synapses ◽  
Organotypic Slice Culture ◽  
Transmitter Receptors

AbstractVertical Slices of postnatal day 6 (P6) rat retina were cut and cultured using the roller-tube technique. The organotypic differentiation during a culture period of up to 30 days has been described in a previous study (Feigenspan et al., 1993a). Here we concentrated on the synaptic organization in the retinal slice culture. Electron microscopy revealed the presence of ribbon synapses in the outer plexiform layer and conventional and ribbon syanpses in the inner plexiform layer. Immunofluroscence with antibodies that recognize specific subunits of GABAA or glycine receptors revealed a punctuate distribution of the receptors. They were aggregated in “hot spots” that correspond to a concentration of receptors at postsynaptic sites. Different isoforms of GABAA and glycine receptors occured in the slice cultures. The experiments show that there is a differentiation of synapses and a diversity of transmitter receptors in the slice cultures that is comparable to the in vivo retina.

scholarly journals Heme oxygenase-1 contributes to pathology associated with thrombin-induced striatal and cortical injury in organotypic slice culture

2010 ◽  
Vol 1347 ◽  
pp. 170-178 ◽  
Author(s):  
Masatoshi Ohnishi ◽  
Hiroshi Katsuki ◽  
Kazuhiro Unemura ◽  
Yasuhiko Izumi ◽  
Toshiaki Kume ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Slice Culture ◽  
Cortical Injury ◽  
Organotypic Slice Culture
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