Demethylation of tertiary amines by a reconstituted cytochrome P-450 enzyme system: Kinetics of oxygen consumption and hydrogen peroxide formation

1989 ◽  
Vol 37 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Otto Ristau ◽  
Horst Rein ◽  
Klaus Ruckpaul ◽  
Dana M. Wagnerova
Keyword(s):  
Hydrogen Peroxide ◽  
Oxygen Consumption ◽  
Enzyme System ◽  
Tertiary Amines ◽  
Peroxide Formation ◽  
Hydrogen Peroxide Formation ◽  
Kinetics Of ◽  
Cytochrome P 450

scholarly journals Hydrogen Peroxide Formation by Reaction of Peroxynitrite with HEPES and Related Tertiary Amines

1998 ◽  
Vol 273 (21) ◽  
pp. 12716-12724 ◽  
Author(s):  
Michael Kirsch ◽  
Elena E. Lomonosova ◽  
Hans-Gert Korth ◽  
Reiner Sustmann ◽  
Herbert de Groot
Keyword(s):  
Hydrogen Peroxide ◽  
Tertiary Amines ◽  
Peroxide Formation ◽  
Hydrogen Peroxide Formation

A New and Rapid Method for the Determination of Glucose by Measurement of Rate of Oxygen Consumption

1968 ◽  
Vol 14 (2) ◽  
pp. 116-131 ◽  
Author(s):  
Arnold Henry Kadish ◽  
Robert L Litle ◽  
James C Sternberg
Keyword(s):  
Hydrogen Peroxide ◽  
Oxygen Consumption ◽  
Glucose Oxidase ◽  
Oxygen Sensor ◽  
Direct Measure ◽  
Peroxide Formation ◽  
Glucose Levels ◽  
Rate Of Oxygen Consumption ◽  
Hydrogen Peroxide Formation

Abstract Glucose levels in serum, plasma, and urine are determined rapidly and conveniently by a new glucose oxidase method employing a polarographic oxygen sensor with a circuit modified to record the rate of oxygen consumption. The maximum apparent rate of oxygen consumption relative to the rate obtained with a glucose standard provides a direct measure of the glucose level in the sample; results are obtainable within 20 sec. after sample (100 µl.) addition and within 3 min. after a blood sample is withdrawn from a patient. Interferences associated with prior colorimetric glucose oxidase methods are avoided by measuring oxygen consumption instead of hydrogen peroxide formation. The method is described and results are presented showing a standard deviation of less than 1.5% on replicate determinations and a bias of 1% with respect to data obtained on the same samples by the automated ferricyanide method.

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