Incidence of chromosome aberrations in mammalian sperm stained with Hoechst 33342 and UV-laser irradiated during flow sorting

Development of flow cytometry for sorting mammalian sperm according to their sex chromosomes began in the late 1970s and early 1980s. This technology, which has recently been commercialized for bovine sperm, is based on the differences in DNA content between X- and Y-chromosome-bearing sperm. Under ideal conditions, 5000 live bovine sperm of each sex can be sorted per second at 90% accuracy. Pregnancy rates of 50% have been achieved routinely in well-managed heifers with sex-sorted, cryopreserved bovine sperm compared to 60–80% with unsexed control sperm. About 90% of offspring have been of the selected sex. Sorting sperm according to sex chromosome content is similarly successful in many other mammals including exotic species, but sorting efficiencies are somewhat less for sperm from some species. Key words: Mammals, sex chromosomes, flow cytometer, cell sorter, DNA content, X and Y sperm, Hoechst 33342

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Hoechst 33342: The dye that enabled differentiation of living X-and Y-chromosome bearing mammalian sperm

Theriogenology ◽

10.1016/j.theriogenology.2008.09.023 ◽

2009 ◽

Vol 71 (1) ◽

pp. 11-21 ◽

Cited By ~ 36

Author(s):

D.L. Garner

Keyword(s):

Y Chromosome ◽

Hoechst 33342 ◽

Mammalian Sperm

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Studies on the mechanism of printing film-coated tablets containing titanium dioxide in the film by using UV laser irradiation

Drug Development and Industrial Pharmacy ◽

10.1080/03639040903213725 ◽

2009 ◽

Vol 00 (00) ◽

pp. 090915102728058-8

Author(s):

Yoshiteru Kato ◽

Yasuhiko Nakashima ◽

Naoki Shino ◽

Koichi Sasaki ◽

Akihiro Hosokawa ◽

...

Keyword(s):

Titanium Dioxide ◽

Laser Irradiation ◽

Uv Laser

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UV LASER-DRIVEN SHOCK-WAVE EXPERIMENTS AT ULTRAHIGH-PRESSURES UP TO 5 TPa

Le Journal de Physique Colloques ◽

10.1051/jphyscol:1984856 ◽

1984 ◽

Vol 45 (C8) ◽

pp. C8-305-C8-308 ◽

Cited By ~ 1

Author(s):

F. Cottet ◽

M. Hallouin ◽

J. P. Romain ◽

R. Fabbro ◽

B. Faral

Keyword(s):

Shock Wave ◽

Uv Laser

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Bestimmung der DNA-Schädigung in vitro

Nuklearmedizin ◽

10.1055/s-0038-1626526 ◽

2010 ◽

Vol 49 (S 01) ◽

pp. S64-S68

Author(s):

E. Dikomey

Keyword(s):

Dna Damage ◽

Cell Lines ◽

Chromosome Aberrations ◽

Genetic Factors ◽

Double Strand Breaks ◽

Strand Breaks ◽

Cell Inactivation ◽

Repair Mechanisms ◽

Break Repair

SummaryIonising irradiation acts primarily via induction of DNA damage, among which doublestrand breaks are the most important lesions. These lesions may lead to lethal chromosome aberrations, which are the main reason for cell inactivation. Double-strand breaks can be repaired by several different mechanisms. The regulation of these mechanisms appears be fairly different for normal and tumour cells. Among different cell lines capacity of doublestrand break repair varies by only few percents and is known to be determined mostly by genetic factors. Knowledge about doublestrand break repair mechanisms and their regulation is important for the optimal application of ionising irradiation in medicine.

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