Studies on the utilization of a plant SCE test in detecting potential mutagenic agents

The aim of this study is to investigate the effects of the storax balsam, which is a kind of sweet gum obtained from the Liquidambar orientalis Mill trees, on cell viability, cytotoxicity and genotoxicity in human lymphocyte in vitro. We studied the genotoxic effects of the extract of storax balsam (SE) using sister chromatid exchange (SCE) test system. Also the cytotoxic and inhibitory effects on cell proliferation of SE were evaluated using lactate dehydrogenase (LDH) assay and cell proliferation (WST-1) assay. The SCE frequency was increased when the cells were treated with 1.6 and 4.0 µg/mL SE concentrations ( p < 0.05). Moreover, treatment of the cells with the same concentrations significantly depleted the cell number at 24th and 48th hours and elevated the LDH levels ( p < 0.05) at 48th hour. These results suggest that SE can be used as an alternative antibacterial and antipathogenic agent due to its cytotoxic and genotoxic effects.

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In VivoCytogenetic Studies on Aspartame

Comparative and Functional Genomics ◽

10.1155/2010/605921 ◽

2010 ◽

Vol 2010 ◽

pp. 1-4 ◽

Cited By ~ 8

Author(s):

Entissar S. AlSuhaibani

Keyword(s):

Bone Marrow ◽

Sister Chromatid ◽

Chromosome Aberrations ◽

Bone Marrow Cells ◽

Sister Chromatid Exchanges ◽

Artificial Sweetener ◽

Chromatid Exchanges ◽

Sce Test ◽

Marrow Cells

Aspartame (a-Laspartyl-L-phenylalanine 1-methylester) is a dipeptide low-calorie artificial sweetener that is widely used as a nonnutritive sweetener in foods and drinks. The safety of aspartame and its metabolic breakdown products (phenylalanine, aspartic acid and methanol) was investigatedin vivousing chromosomal aberration (CA) test and sister chromatid exchange (SCE) test in the bone marrow cells of mice. Swiss Albino male mice were exposed to aspartame (3.5, 35, 350 mg/kg body weight). Bone marrow cells isolated from femora were analyzed for chromosome aberrations and sister chromatid exchanges. Treatment with aspartame induced dose dependently chromosome aberrations at all concentrations while it did not induce sister chromatid exchanges. On the other hand, aspartame did not decrease the mitotic index (MI). However, statistical analysis of the results show that aspartame is not significantly genotoxic at low concentration.

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Evaluation of experimental parameters in an S9/human leukocyte SCE test with cyclophosphamide

Mutation Research/Environmental Mutagenesis and Related Subjects ◽

10.1016/0165-1161(81)90261-2 ◽

1981 ◽

Vol 85 (6) ◽

pp. 447-448

Author(s):

S. Madle

Keyword(s):

Human Leukocyte ◽

Experimental Parameters ◽

Sce Test

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Sister chromatid exchange in selected horse breeds (<i>Equus caballus</i>)

Archives Animal Breeding ◽

10.5194/aab-54-107-2011 ◽

2011 ◽

Vol 54 (2) ◽

pp. 107-114 ◽

Cited By ~ 1

Author(s):

E. Wójcik ◽

E. Smalec ◽

A. Danielewicz

Keyword(s):

Sister Chromatid ◽

Sister Chromatid Exchange ◽

Chromosome Instability ◽

Age Groups ◽

Peripheral Blood Lymphocytes ◽

Mean Frequency ◽

Males And Females ◽

The Mean ◽

Sce Test

Abstract. In studies of chromosome instability, the sister chromatid exchange (SCE) test is a particularly sensitive cytogenetic assay for detecting DNA damage. SCE tests of chromosome instability were performed in the group of 6 horse breeds (Pure-bred Arabian, Malapolski horse, Polish noble half-bred, Polish cold-blooded, Hucul and Polish Konik). The chromosome preparations were obtained from our in vitro culture of peripheral blood lymphocytes stained using the FPG technique. The mean number of SCEs/cell in the analysed population of horses was 5.14±1.44. The mean frequency of SCEs in the 6 analysed horse breeds varied depending on the breed. Statistically significant differences were observed between the horse breeds (P<0.01). No statistically significant differences in the number of SCEs per cell were found between the males and females (5.10±1.34 and 5.20±1.52, respectively). The horses were also assessed for the number of SCEs/cell in relation to the age of the animals. The differences between the age groups were statistically significant (P<0.01).

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