Immunocytochemical analysis of mitogen responses of carp (Cyprinus carpio L.) peripheral blood leucocytes

1994 ◽  
Vol 42 (2) ◽  
pp. 209-219 ◽  
Author(s):  
J.C.E. Koumans-van Diepen ◽  
E.G.M. Harmsen ◽  
J.H.W.M. Rombout
Keyword(s):  
Cyprinus Carpio ◽  
Peripheral Blood ◽  
Immunocytochemical Analysis ◽  
Carp Cyprinus Carpio ◽  
Peripheral Blood Leucocytes

In vitro effect of lentinan on the activation of immunological cells in Cyprinus carpio

2009 ◽  
Vol 6 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Zhang Liu ◽  
Cao Li-Ping ◽  
Ding Wei-Dong ◽  
Galina Jeney ◽  
Xu Pao ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Peripheral Blood ◽  
Head Kidney ◽  
Burst Activity ◽  
Griess Reaction ◽  
Nitroblue Tetrazolium Reduction ◽  
Diphenyl Tetrazolium Bromide ◽  
Vitro Effect ◽  
Peripheral Blood Leucocytes

AbstractMacrophages from the head kidney (HK) and peripheral blood leucocytes were isolated fromCyprinus carpio by Percoll gradient density centrifugation, cultured in vitro and exposed to different concentrations of the immunomodulator lentinan. To evaluate the immunostimulating effects of lentinan, proliferation of the peripheral blood leucocytes, respiratory burst of macrophages and interleukin-1β (IL-1β) gene mRNA expression of macrophages were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], NBT (nitroblue tetrazolium) reduction, Griess reaction and real-time polymerase chain reaction (PCR). Results showed that proliferation of peripheral blood leucocytes after 24 h incubation by the induction of lentinan at 100 and 1000 μg/ml was markedly stimulated. Lentinan at 1, 100 and 1000 μg/ml could significantly induce superoxide anions in macrophages. Production of nitric oxide by HK macrophages after 96 h incubation by lentinan showed that it had no conspicuous effect on nitrogen burst activity of macrophages. Moreover, it inhibited the nitrogen burst activity at higher doses. The expression of IL-1β in the HK macrophages after 24 h of polysaccharide stimulation showed that lentinan stimulated IL-1β expression in the head kidney in carp. Lentinan can modulate the immune response of C. carpio.

RP1 Granulocytes in carp (Cyprinus carpio L.) peripheral blood are responsible for natural cytotoxicity

1997 ◽  
Vol 21 (2) ◽  
pp. 222
Author(s):  
Chihaya Nakayasu ◽  
Satoshi Hasegawa ◽  
Tomoyasu Yoshitomi ◽  
Nobuaki Okamoto
Keyword(s):  
Cyprinus Carpio ◽  
Peripheral Blood ◽  
Natural Cytotoxicity ◽  
Carp Cyprinus Carpio

Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)

2019 ◽  
pp. 339-349
Author(s):  
Armando Elizalde-Velázquez ◽  
Martínez-Rodríguez Héctor ◽  
Marcela Galar-Martínez ◽  
Luis Gerardo Bernadac Villegas ◽  
Octavio Dublán-García ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Peripheral Blood ◽  
Genotoxic Effect ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

The Growth of Herpesvirus mulatto in Human Fibroblast

1974 ◽  
Vol 32 ◽  
pp. 244-245
Author(s):  
Glennelle Washington ◽  
Philip P. McGrath ◽  
Peter R. Graze ◽  
Ivor Royston
Keyword(s):  
Rhesus Monkey ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Peripheral Blood ◽  
Human Fibroblast ◽  
Rhesus Monkeys ◽  
Human Fibroblasts ◽  
Electron Microscopic ◽  
Specific Antisera ◽  
Peripheral Blood Leucocytes

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

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