7. Polymerase chain reaction analysis for specific HTLV-1 sequences from cerebrospinal fluid and peripheral blood cells in multiple sclerosis patients from Sardinia

1991 ◽  
Vol 34 (2-3) ◽  
pp. 244
Author(s):  
Maria Giovanna Marrosu ◽  
Anna Paola Mazzoleni ◽  
Silvana Galantuomo ◽  
Antonello Melis ◽  
Francesco Muntoni ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Polymerase Chain Reaction Analysis ◽  
Peripheral Blood Cells ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Multiple Sclerosis Patients

Failure to Detect Measles Virus Rna, by Reverse Transcription-Polymerase Chain Reaction, in Peripheral Blood Leucocytes of Patients with Multiple Sclerosis

1996 ◽  
Vol 1 (4) ◽  
pp. 204-206 ◽  
Author(s):  
B. Brankin ◽  
M. Osman ◽  
L. Herlihy ◽  
S.A. Hawkins ◽  
S.L. Cosby
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Measles Virus ◽  
Chain Reaction ◽  
Specific Primers ◽  
Polymerase Chain ◽  
Multiple Sclerosis Patients ◽  
Peripheral Blood Leucocytes

We have examined peripheral blood leucocytes (PBLs) from 17 multiple sclerosis patients, two patients with rheumatoid arthritis, one case of acute childhood measles and one case of subacute sclerosing panencephalitis, as well as 19 healthy adult controls for measles virus (MV) RNA, by the technique of reverse transcription-polymerase chain reaction. MV nucleocapsid gene specific primers were used to amplify all PBL-derived cDNA samples. These proved to be negative with the exception of the sample derived from the acute measles case. Selected cases were examined further, using fusion gene and matrix gene specific primers. MV RNA could not be detected.

scholarly journals New sensitive method for the detection of the A3243G mutation of human mitochondrial deoxyribonucleic acid in diabetes mellitus patients by ligation-mediated polymerase chain reaction

1998 ◽  
Vol 44 (10) ◽  
pp. 2088-2093 ◽  
Author(s):  
Michiyo Urata ◽  
Machiko Wakiyama ◽  
Masanori Iwase ◽  
Makoto Yoneda ◽  
Sachiko Kinoshita ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Polymerase Chain Reaction ◽  
Conventional Method ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Nucleotide Position ◽  
Peripheral Blood Cells ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Sensitive Method

Abstract An adenine-to-guanine mutation at nucleotide position (np) 3243 in the mitochondrial tRNALeu(UUR) gene is closely associated with various clinical phenotypes of diabetes mellitus. Because the mutation creates a new restriction site for the restriction enzyme ApaI, the mutation is usually detected and quantified by ApaI cleavage of the PCR products including np 3243. The sensitivity of the conventional method is, however, 5–10% heteroplasmy. The percentage of heteroplasmy of the mutation is usually highest in the affected tissues and is much lower in peripheral blood cells, which are used most frequently for the analysis. The sensitivity of the conventional method, however, is not sufficient to detect the mutation from peripheral blood cells. Utilizing ligation-mediated polymerase chain reaction, we have developed a feasible and sensitive method to detect 0.01% heteroplasmy of the 3243 mutation in peripheral leukocytes.

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