A direct viable count method for the enumeration of attached bacteria and assessment of biofilm disinfection

1993 ◽  
Vol 17 (3) ◽  
pp. 167-180 ◽  
Author(s):  
Feipeng P. Yu ◽  
Barry H. Pyle ◽  
Gordon A. McFeters
Keyword(s):  
Viable Count ◽  
Count Method ◽  
Attached Bacteria

Distribution of heterotrophic bacteria in relation to the concentration of particulate organic matter in seawater

1983 ◽  
Vol 29 (5) ◽  
pp. 570-575 ◽  
Author(s):  
Kimio Fukami ◽  
Usio Simidu ◽  
Nobuo Taga
Keyword(s):  
Organic Matter ◽  
Particulate Organic Matter ◽  
Heterotrophic Bacteria ◽  
Viable Count ◽  
Plate Count ◽  
Free Living ◽  
Count Method ◽  
Attached Bacteria ◽  
Geographical Scale ◽  
Living Bacteria

The relationship between the number of bacteria and the concentration of particulate organic carbon (POC) in seawater was investigated. In coastal seawater in summer, the POC concentration showed better correlation to the density of bacteria obtained by the viable plate count method (viable count, V.C.) than by the total direct count method (total count, T.C.). The number of attached bacteria (A) showed significant fluctuation, both laterally on a geographical scale and vertically in the water column; on the other hand, the number of free-living bacteria (F) was relatively constant. The POC concentration had a much higher correlation with A (r = 0.8795) than with T.C. (r = 0.7339), and had a low correlation with F (r = 0.6935). Moreover, a very good correlation was observed between the density of bacteria obtained by V.C. and A (r = 0.9153). These results indicate that when the concentration of particulate organic matter (POM) increases, some free-living bacteria become attached to POM, and grow on the surface of POM. These communities of attached bacteria have the ability to make colonies on plate media and can be counted as the "viable plate count."

The optimization and application of two direct viable count methods for bacteria in distributed drinking water

1994 ◽  
Vol 40 (10) ◽  
pp. 830-836 ◽  
Author(s):  
Josée Coallier ◽  
Michèle Prévost ◽  
Annie Rompré ◽  
Daniel Duchesne
Keyword(s):  
Drinking Water ◽  
Nalidixic Acid ◽  
Bacterial Population ◽  
Laboratory Strain ◽  
Viable Count ◽  
Tetrazolium Chloride ◽  
Count Method ◽  
Exogenous Substrate ◽  
In Situ Conditions

The optimal incubation conditions for the direct viable count method with nalidixic acid were determined. They do not differ from those proposed in the literature for a laboratory strain and a mixed bacterial population isolated from drinking water. The direct viable count method with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was performed under in situ conditions. The bacteria were incubated with CTC at a concentration of 1 mM for 4–6 h at the temperature of the water in the pipes and without the addition of an exogenous substrate. The results obtained for a laboratory strain using the two direct count methods were similar. However, for a mixed bacterial population, the counts were always higher with the CTC method than with the nalidixic acid method.Key words: drinking water, CTC, nalidixic acid, direct viable count.

An improved direct viable count method for aquatic bacteria

1984 ◽  
Vol 102 (1) ◽  
pp. 117-122
Author(s):  
Kazuhiro Kogure ◽  
Usio Simidu ◽  
Nobuo Taga
Keyword(s):  
Viable Count ◽  
Aquatic Bacteria ◽  
Count Method

scholarly journals A most probable number method for estimating small numbers of campylobacters in water

1982 ◽  
Vol 89 (2) ◽  
pp. 185-190 ◽  
Author(s):  
F. J. Bolton ◽  
P. M. Hinchliffe ◽  
D. Coates ◽  
L. Robertson
Keyword(s):  
Campylobacter Jejuni ◽  
Water Samples ◽  
Enrichment Culture ◽  
Viable Count ◽  
Most Probable Number ◽  
Raw Water ◽  
Culture Methods ◽  
Probable Number ◽  
Count Method ◽  
Epidemiological Tool

SUMMARYA most probable number (MPN) method capable of estimating as few as ten campylobacters per 100 ml of water is described. The method gave results close to those obtained by the viable count method of Miles, Misra & Irwin (1938) with graded suspensions of Campylobacter jejuni. The method was used to test raw water samples: counts were obtained ranging from 10 to 230 campylobacters per 100 ml for 11 of 49 coastal and estuary water samples, and from 10 to 36 campylobacters per 100 ml for 7 of 44 river samples. Campylobacters were isolated from an additional 24 of the ‘negative’ samples by testing 200 ml volumes by glass microfibre filtration and enrichment culture methods. The MPN method should prove to be a useful epidemiological tool particularly suited to the enumeration of campylobacters in particulate fluids.

scholarly journals Postantibiotic Effects of Antituberculosis Agents Alone and in Combination

2001 ◽  
Vol 45 (12) ◽  
pp. 3631-3634 ◽  
Author(s):  
Chiu-Yeung Chan ◽  
Carrie Au-Yeang ◽  
Wing-Wai Yew ◽  
Mamie Hui ◽  
Augustine F. B. Cheng
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Culture System ◽  
Scientific Basis ◽  
Viable Count ◽  
First Line ◽  
Count Method ◽  
Antimycobacterial Agents ◽  
Antituberculosis Chemotherapy

ABSTRACT The postantibiotic effects (PAEs) of seven antimycobacterial agents, tested at their respective peak concentrations in serum alone and in different combinations, against Mycobacterium tuberculosis ATCC 27294 were studied with a radiometric culture system in parallel with the viable count method. Rifampin gave the longest PAE (67.8 h) among the drugs used alone, and combinations of first-line drugs generally gave PAEs longer than 120 h. The data obtained might help provide a better understanding of the scientific basis of intermittently administered antituberculosis chemotherapy.

scholarly journals Enumeration and characterization of bacteria in mineral water by improved direct viable count method

1999 ◽  
Vol 86 (5) ◽  
pp. 841-850 ◽  
Author(s):  
S. Guyard ◽  
P. Mary ◽  
C. Defives ◽  
J. P. Hornez
Keyword(s):  
Mineral Water ◽  
Viable Count ◽  
Count Method
Sign in / Sign up

Export Citation Format

Share Document