Sendai virus replication in Friend erythroleukemia cells. I. Acutely and persistently infected cells become resistant to virus-induced lysis

A gradual increase in the level of 2′,5′-oligoadenylate synthetase takes place in Friend erythroleukemia cells after a shiftdown in the rate of cell growth. The increase is about 5-fold after entry of cells into the stationary phase of growth, but much higher (25-fold) when reduction in growth accompanies cell differentiation. In the latter case, the enzyme increase is similar to that which can be induced in these cells by exogenous interferon (IFN). The increase in 2′,5′-oligoadenylate synthetase was shown to be due to a spontaneous secretion of IFN by the cells themselves: it is completely abolished if antiserum to murine type I IFN is added to the culture medium. In attempts to isolate some of this spontaneously secreted IFN, we show that it is stable at pH 2, not neutralized by antiserum to type II IFN, and that it also differs from the known IFN species induced by Sendai virus in Friend cells. The major component of this spontaneously secreted IFN is 20,000Mrand differs from the corresponding virus-induced 20,000-MrIFN by its lower affinity for antiserum to type I IFN and its antigenic characterization as β-murine IFN. The major component of the spontaneous IFN also exhibits a higher ratio of antigrowth to antiviral activity than the Sendai-induced IFNs. We suggest that Friend cells produce this specific type of IFN for the regulation of their growth and differentiation.

Download Full-text

The relationship between prostaglandins and virus replication: Endogenous prostaglandin synthesis during infection and the effect of exogenous PGA on virus production in different cell lines and in persistently infected cells

Prostaglandins ◽

10.1016/0090-6980(83)90038-2 ◽

1983 ◽

Vol 25 (3) ◽

pp. 353-364 ◽

Cited By ~ 11

Author(s):

M.G. Santoro ◽

A. Benedetto ◽

S. Zaniratti ◽

E. Garaci ◽

B.M. Jaffe

Keyword(s):

Cell Lines ◽

Virus Replication ◽

Virus Production ◽

Prostaglandin Synthesis ◽

Persistently Infected ◽

Endogenous Prostaglandin ◽

Infected Cells ◽

The Relationship

Download Full-text

Vaccinia virus DNA sequences in the nucleus of persistently infected Friend erythroleukemia cells

Virus Research ◽

10.1016/0168-1702(86)90020-1 ◽

1986 ◽

Vol 5 (2-3) ◽

pp. 221-234 ◽

Cited By ~ 7

Author(s):

Kristina M. Obom ◽

Steven W. Popple ◽

J.Gilbert Holland ◽

Beatriz G.-T. Pogo ◽

Charlotte Friend

Keyword(s):

Vaccinia Virus ◽

Dna Sequences ◽

Persistently Infected ◽

Erythroleukemia Cells ◽

Friend Erythroleukemia Cells

Download Full-text

Early Stage of Establishment of Persistent Sendai Virus Infection: Unstable Dynamic Phase and Then Selection of Viruses Which Are Tightly Cell Associated, Temperature Sensitive, and Capable of Establishing Persistent Infection

Journal of Virology ◽

10.1128/jvi.78.21.11939-11951.2004 ◽

2004 ◽

Vol 78 (21) ◽

pp. 11939-11951 ◽

Cited By ~ 6

Author(s):

Morihiro Ito ◽

Taijiro Takeuchi ◽

Machiko Nishio ◽

Mitsuo Kawano ◽

Hiroshi Komada ◽

...

Keyword(s):

Virus Infection ◽

Cell Lines ◽

Temperature Sensitivity ◽

Persistent Infection ◽

Sendai Virus ◽

Early Stage ◽

Persistently Infected ◽

Dynamic Phase ◽

M Proteins ◽

Infected Cells

ABSTRACT We obtained 157 cloned cell lines persistently infected with Sendai virus; these cell lines were generated independently of each other. Infectious viruses could be isolated from 123 of these cloned cell lines by inoculation of culture fluids or infected cells into embryonated eggs. The majority of the viruses carried by cells persistently infected with viruses showed high cytotoxicity and did not have the ability to establish persistent infection. The association of carried virus with cells became stronger and virus isolation correspondingly became more difficult as cells persistently infected with virus were subcultured. Viruses derived from virus-infected cells eventually acquired the ability to establish persistent infection, although the ways in which the viruses acquired this ability varied. The viruses also acquired temperature sensitivity as persistently infected cells were subcultured. First, the hemagglutinin-neuraminidase and M proteins acquired temperature sensitivity, and then the polymerase(s) did so. The M proteins were localized in the nuclei of cells infected with cloned viruses that had the ability to establish persistent infection. Cells infected with viruses capable of establishing persistent infection showed no or slight staining by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling. Specific amino acid substitutions accumulated in the M protein and the L protein as virus-infected cells were subcultured. This study shows that there is an unstable dynamic phase at an early stage of the establishment of persistent Sendai virus infection (steady state), and then viruses capable of establishing persistent infection are selected.

Download Full-text

Inhibition of Borna disease virus replication by ribavirin in persistently infected cells

Archives of Virology ◽

10.1007/s007050050440 ◽

1998 ◽

Vol 143 (10) ◽

pp. 2039-2044 ◽

Cited By ~ 25

Author(s):

T. Mizutani ◽

H. Inagaki ◽

K. Araki ◽

H. Kariwa ◽

J. Arikawa ◽

...

Keyword(s):

Disease Virus ◽

Virus Replication ◽

Borna Disease Virus ◽

Persistently Infected ◽

Infected Cells ◽

Borna Disease

Download Full-text

Initial Characterization of a Spontaneous Interferon Secreted During Growth and Differentiation of Friend Erythroleukemia Cells

Molecular and Cellular Biology ◽

10.1128/mcb.2.12.1472-1480.1982 ◽

1982 ◽

Vol 2 (12) ◽

pp. 1472-1480

Author(s):

Miriam Friedman-Einat Michel Revel ◽

Adi Kimchi

Keyword(s):

Sendai Virus ◽

Type I ◽

Type I Ifn ◽

Oligoadenylate Synthetase ◽

Erythroleukemia Cells ◽

Antigenic Characterization ◽

Friend Cells ◽

Friend Erythroleukemia Cells ◽

Spontaneous Secretion

A gradual increase in the level of 2′,5′-oligoadenylate synthetase takes place in Friend erythroleukemia cells after a shiftdown in the rate of cell growth. The increase is about 5-fold after entry of cells into the stationary phase of growth, but much higher (25-fold) when reduction in growth accompanies cell differentiation. In the latter case, the enzyme increase is similar to that which can be induced in these cells by exogenous interferon (IFN). The increase in 2′,5′-oligoadenylate synthetase was shown to be due to a spontaneous secretion of IFN by the cells themselves: it is completely abolished if antiserum to murine type I IFN is added to the culture medium. In attempts to isolate some of this spontaneously secreted IFN, we show that it is stable at pH 2, not neutralized by antiserum to type II IFN, and that it also differs from the known IFN species induced by Sendai virus in Friend cells. The major component of this spontaneously secreted IFN is 20,000 M r and differs from the corresponding virus-induced 20,000- M r IFN by its lower affinity for antiserum to type I IFN and its antigenic characterization as β-murine IFN. The major component of the spontaneous IFN also exhibits a higher ratio of antigrowth to antiviral activity than the Sendai-induced IFNs. We suggest that Friend cells produce this specific type of IFN for the regulation of their growth and differentiation.

Download Full-text