Oral immunization of foxes with avirulent rabies virus mutants

METHOD OF DETERMINING THE IMMUNOGENIC ACTIVITY OF LIVE VACCINE AGAINST RABIES IN WILD ANIMALS IN MICE

Innovations and Food Safety ◽

10.31677/2311-0651-2019-24-2-53-57 ◽

2020 ◽

pp. 53-57

Author(s):

V. A. Babak ◽

A. A. Gusev ◽

I. A. Puntus ◽

A. S. Smailova

Keyword(s):

Infection Control ◽

Rabies Virus ◽

Experimental Infection ◽

Oral Immunization ◽

Live Vaccine ◽

Wild Animals ◽

Method Of Evaluation

The results of alternative studies on the immunogenic activity of live rabies vaccines for oral immunization of wild carnivorous animals are presented. The method of evaluation of immunogenicity using a model of oral immunization in mice with experimental infection control rabies virus CVS in the dose of 10–100 MLD50/0,03 ml. Once entered immunizing dose for white mice, weighing 12–14 g were 56.200 MLD50, the titers of VNA ranged from 1:6 to 1:16 (3,0–4,0 log2) and above.

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PREREQUISITES FOR ORAL IMMUNIZATION OF FREE-RANGING RACCOONS (PROCYON LOTOR) WITH A RECOMBINANT RABIES VIRUS VACCINE: STUDY SITE ECOLOGY AND BAIT SYSTEM DEVELOPMENT

Journal of Wildlife Diseases ◽

10.7589/0090-3558-28.1.64 ◽

1992 ◽

Vol 28 (1) ◽

pp. 64-79 ◽

Cited By ~ 17

Author(s):

C. P. Hable ◽

A. N. Hamir ◽

D. E. Snyder ◽

R. Joyner ◽

J. French ◽

...

Keyword(s):

Rabies Virus ◽

Virus Vaccine ◽

Procyon Lotor ◽

System Development ◽

Oral Immunization ◽

Vaccine Study ◽

Free Ranging ◽

Bait System ◽

Site Ecology

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Genetic characterisation of the rabies virus vaccine strains used for oral immunization of foxes in Poland to estimate the effectiveness of vaccination

Archives of Virology ◽

10.1007/s00705-014-2269-y ◽

2014 ◽

Vol 160 (2) ◽

pp. 509-515 ◽

Cited By ~ 3

Author(s):

Anna Orłowska ◽

Jan Franciszek Żmudziński

Keyword(s):

Rabies Virus ◽

Virus Vaccine ◽

Oral Immunization ◽

Genetic Characterisation

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Oral Vaccination of Mice with Adenoviral Vectors Is Not Impaired by Preexisting Immunity to the Vaccine Carrier

Journal of Virology ◽

10.1128/jvi.77.20.10780-10789.2003 ◽

2003 ◽

Vol 77 (20) ◽

pp. 10780-10789 ◽

Cited By ~ 101

Author(s):

Z. Q. Xiang ◽

G. P. Gao ◽

A. Reyes-Sandoval ◽

Y. Li ◽

J. M. Wilson ◽

...

Keyword(s):

Rabies Virus ◽

Neutralizing Antibody ◽

Oral Immunization ◽

Adenoviral Vectors ◽

Oral Vaccination ◽

Newborn Mice ◽

Serotype 5 ◽

Antibody Titers ◽

Vaccine Carrier ◽

Mucosal Secretions

ABSTRACT E1 deletion adenoviral vectors of the human serotype 5 (AdHu5) and the chimpanzee serotype 68 (AdC68) expressing the rabies virus glycoprotein (rab.gp) were tested for induction of transgene product-specific antibodies upon intranasal or oral immunization of newborn mice. Both vectors induced antibodies to rabies virus that could be detected in serum and mucosal secretions. Serum rabies virus-neutralizing antibody titers sufficed to protect neonatally vaccinated mice against a subsequent challenge with rabies virus. The efficacy of the AdHu5rab.gp vector given orally to newborn mice born to AdHu5-immune dams was not impaired by maternally transferred antibodies to the vaccine carrier.

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Monitoring of rabies in wild animals in the Kirov region after oral immunization

Voprosy Virusologii ◽

10.18821/0507-4088-2016-61-4-186-192 ◽

2016 ◽

Vol 61 (4) ◽

pp. 186-192 ◽

Cited By ~ 1

Author(s):

O. N. Zaykova ◽

T. V. Grebennikova ◽

A. L. Elakov ◽

K. S. Kochergin-Nikitsky ◽

T. I. Aliper ◽

...

Keyword(s):

Rabies Virus ◽

Vaccine Strain ◽

Oral Vaccine ◽

Molecular Genetic ◽

Genetic Research ◽

Oral Immunization ◽

Molecular Genetic Analysis ◽

Wild Isolate ◽

Field Isolates ◽

Pcr Techniques

This work presents the results of the molecular genetic research on genomes of field isolates of the rabies virus circulating in the territory of the Kirov region in order to analyze the phylogenetic relationship between the wild isolate genomes and to determine the possible reversion of the vaccine strain of the rabies virus used in the oral vaccine to virulent variant. We studied 24 brain samples from wild carnivores shot after oral immunization of the area with Rabivak-O/333. A bait with the vaccine provided by the Veterinary Service of the Kirov was also studied. All samples were found to be positive for the presence of the rabies virus as established by FAT and RT-PCR techniques. Phylogenetic analysis of N genome fragments of the rabies virus showed that the field isolates from the Kirov regions were genetically close to the field isolates from Buryatia 2012. Analysis of G genome fragments showed that the Kirov field isolates were close to the isolates from Lipetsk (2011), as well as to the Ukrainian isolates (2006 and 2010). Molecular genetic analysis of the gene fragments N and G for the field isolates and fragments of the genome of the rabies virus vaccine did not reveal any reversion to the virulent vaccine strain.

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