A variable region of Anticarsia gemmatalis nuclear polyhedrosis virus contains tandemly repeated DNA sequences

1996 ◽  
Vol 41 (2) ◽  
pp. 123-132 ◽  
Author(s):  
Alejandra Garcia-Maruniak ◽  
Octavio H.O Pavan ◽  
James E Maruniak
1992 ◽  
Vol 27 (2) ◽  
pp. 126-134 ◽  
Author(s):  
S. Y. Young ◽  
W. C. Yearian

Nabis roseipennis Reuter nymphs that preyed on larvae of the nuclear polyhedrosis virus (NPV) infected velvetbean caterpillar, Anticarsia gemmatalis (Hübner), excreted the virus (AgNPV) for several days thereafter. Based on bioassays, fifth instar and second instar nymphs excreted 84.7 × 105 and 9.7 × 105 polyhedral inclusion bodies (PIB) per nymph, respectively. The AgNPV-contaminated nymphs effectively disseminated the virus via the feces over soybean plants where it served as inoculum to initiate disease in larval populations of A. gemmatalis caged in the field. Larval mortality from AgNPV ranged from 11.4 to 48.5% over treatments in two tests. Larval mortality in treatments where the source of virus inoculum was AgNPV-contaminated fifth instar nymphs was similar to that in treatments where the source of viral inoculum was diseased larvae. Larval mortality resulting from AgNPV dissemination by the nymphs was usually higher in treatments containing fifth instar nymphs than in those with second instar nymphs. Dissemination of NPV by fifth instar nymphs was higher in mixed-age than in uniformed-age A. gemmatalis larval populations. This was not the case with the smaller second instar nymphs.


Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Thomas Schmidt ◽  
Jörg Kudla

Monomers of a major family of tandemly repeated DNA sequences of Antirrhinum majus have been cloned and characterized. The repeats are 163–167 bp long, contain on average 60% A + T residues, and are organized in head-to-tail orientation. According to site-specific methylation differences two subsets of repeating units can be distinguished. Fluorescent in situ hybridization revealed that the repeats are localized at centromeric regions of six of the eight chromosome pairs of A. majus with substantial differences in array size. The monomeric unit shows no homologies to other plant satellite DNAs. The repeat exists in a similar copy number and conserved size in the genomes of six European species of the genus Antirrhinum. Tandemly repeated DNA sequences with homology to the cloned monomer were also found in the North American section Saerorhinum, indicating that this satellite DNA might be of ancient origin and was probably already present in the ancestral genome of both sections. Key words : Antirrhinum majus, satellite DNA, repetitive DNA, methylation, in situ hybridization.


Genomics ◽  
1989 ◽  
Vol 5 (1) ◽  
pp. 153-156 ◽  
Author(s):  
Ulrich Müller ◽  
Marc Lalande ◽  
Timothy A. Donlon ◽  
Michael W. Heartlein

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