Expression of insulin-like growth factor II (IGF-II) and IGF-II, IGF-I and insulin receptors mRNAs in isolated non-parenchymal rat liver cells

1992 ◽  
Vol 14 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Frédérique Zindy ◽  
Eugenia Lamas ◽  
Sylvie Schmidt ◽  
André Kirn ◽  
Christian Brechot
Keyword(s):  
Growth Factor ◽  
Rat Liver ◽  
Insulin Receptors ◽  
Liver Cells ◽  
Insulin Like Growth Factor ◽  
Factor Ii ◽  
Igf I ◽  
Rat Liver Cells

scholarly journals Extinction of insulin-like growth factor II gene expression in intratypic hybrids of rat liver cells.

1993 ◽  
Vol 7 (1) ◽  
pp. 131-141
Author(s):  
R Zarrilli ◽  
S Casola ◽  
A Conti ◽  
C B Bruni ◽  
V Colantuoni
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Rat Liver ◽  
Liver Cells ◽  
Insulin Like Growth Factor ◽  
Factor Ii ◽  
Rat Liver Cells

Regulation of insulin-like-growth-factor-II gene expression in rat liver cells

1992 ◽  
Vol 209 (1) ◽  
pp. 445-452 ◽  
Author(s):  
Raffaele ZARRILLI ◽  
Vittorio COLANTUONI ◽  
Carmelo Bruno BRUNI
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Rat Liver ◽  
Liver Cells ◽  
Insulin Like Growth Factor ◽  
Factor Ii ◽  
Rat Liver Cells

Measurement of Human Igf-II Using Sephacryl Extraction: A Rapid and Reliable Assay Method

1996 ◽  
Vol 33 (3) ◽  
pp. 201-208 ◽  
Author(s):  
Barbara H Mason ◽  
Michele A Tatnell ◽  
Ian M Holdaway
Keyword(s):  
Growth Factor ◽  
Binding Proteins ◽  
Complete Removal ◽  
Assay Method ◽  
Insulin Like Growth Factor ◽  
Serum Concentrations ◽  
Igf Binding Proteins ◽  
Factor Ii ◽  
Igf I ◽  
Igfbp 3

Measurement of insulin-like growth factor II (IGF-II) in human serum is complicated by the presence of IGF binding proteins and usually involves cumbersome extraction procedures followed by radioimmunoassay. We have utilized an extraction process developed for measuring insulin-like growth factor II in ovine serum using Sephacryl HR100, and have applied this to the extraction of human samples followed by radioimmunoassay for human IGF-II. The assay yielded 98% recovery of unlabelled IGF-II, parallelism between dilutions of eluate and the standard curve, complete removal of binding proteins and near-complete removal of IGF-I, and intra- and interassay coefficients of variation of 5% and 9%, respectively. The normal range for serum IGF-II in women was 490–1056 μg/L, and IGF-II levels were positively correlated with serum concentrations of insulin-like growth factor binding protein-3 (IGFBP-3) but not with IGF-I levels. Mean serum concentrations of IGF-II were reduced below normal in a number of hypopituitary patients and children with short stature and IGF-II concentrations in these subjects correlated positively with IGF-I and IGFBP-3. In acromegalic patients IGF-II levels were usually normal and were negatively correlated with IGF-I concentrations. From our experience with the above results the present assay appears particularly suitable for clinical measurements and research projects where high sample throughput is required.

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