High frequency shoot regeneration and Agrobacterium-mediated transformation of chrysanthemum (Dendranthema grandiflora)

Plant Science ◽  
1994 ◽  
Vol 98 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Lori A. Urban ◽  
John M. Sherman ◽  
James W. Moyer ◽  
Margaret E. Daub
Keyword(s):  
Shoot Regeneration ◽  
High Frequency ◽  
Dendranthema Grandiflora ◽  
Agrobacterium Mediated Transformation

Shoot regeneration and Agrobacterium-mediated transformation of Fragaria vesca L.

1996 ◽  
Vol 15 (8) ◽  
pp. 642-646 ◽  
Author(s):  
Iman El Mansouri ◽  
José A. Mercado ◽  
Victoriano Valpuesta ◽  
José M. López-Aranda ◽  
Fernando Pliego-Alfaro ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Fragaria Vesca ◽  
Agrobacterium Mediated Transformation

scholarly journals A Regeneration and Agrobacterium-mediated Transformation System for Genetically Diverse Chrysanthemum Cultivars

1998 ◽  
Vol 123 (2) ◽  
pp. 189-194 ◽  
Author(s):  
John M. Sherman ◽  
James W. Moyer ◽  
Margaret E. Daub
Keyword(s):  
High Frequency ◽  
Callus Formation ◽  
Leaf Explants ◽  
Shoot Elongation ◽  
Neomycin Phosphotransferase ◽  
Cut Flower ◽  
Transformation System ◽  
High Concentration ◽  
Dendranthema Grandiflora ◽  
Rooting Medium

An efficient, high-frequency regeneration and Agrobacterium-mediated transformation system was developed allowing the genetic engineering of three chrysanthemum (Dendranthema grandiflora Tzvelev) cultivars: the formerly recalcitrant and economically important cut-flower mum `Polaris' and two potted mums, `Hekla' and `Iridon'. The regeneration protocol used leaf explants on a sequence of media with four hormone regimes. Explants were first cultured on an embryogenesis-type medium containing a high concentration of 2,4-D, which promoted callus formation. Shoot primordia were induced by culture on medium lacking 2,4-D, followed by shoot elongation on a high-cytokinin plus gibberellic acid medium. Finally, elongated shoots were rooted on a low-auxin rooting medium. Transformed plants of the three cultivars were obtained following co-culture of leaf explants with A. tumefaciens strain EHA 105 harboring the plasmid pBI121 containing genes for neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS). Stable transformation of the three cultivars was verified via GUS assays and Southern analysis.

scholarly journals Callus induction and regeneration of Alkanna orientalis var. Orientalis and A. sieheana

2019 ◽  
Vol 48 (3) ◽  
pp. 633-640 ◽  
Author(s):  
Cenney Yaman ◽  
Serkan Uranbey ◽  
Hussein Abdullah Ahmed ◽  
Sabahattin Özcan ◽  
Osman Tugay ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
High Frequency ◽  
Stem Segment ◽  
Leaf Base ◽  
Cotyledonary Leaf ◽  
Frequency Variations ◽  
Alkanna Orientalis ◽  
Stem Segments

Callus induction and proliferation of Alkanna orientalis var. orientalis and Alkanna sieheana containing valuable alkannin/shikonin (A/S) derivates were investigated using leaf base and stem segment explants. Stem segments and cotyledonary leaf base of both species were cultured on Murashige and Skoog medium fortified with different concentrations of BAP, Kn, NAA, IAA and IBA for callus induction and shoot regeneration. High frequency reproducible, prolific and compact calli formation was obtained from the stem segments of both species in all media tested. The frequency variations of callus induction and shoot regeneration were discussed in terms of different species, plant growth regulators and explant resources. A. orientalis and A. sieheana may be considered to be alternative plants for the A/S production in vitro.

scholarly journals Studies on High Frequency Shoot Regeneration in Sesame (Sesamum indicum L.)

2012 ◽  
Vol 21 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Bangaramma S. Wadeyar ◽  
R. Lokesha
Keyword(s):  
Key Words ◽  
Shoot Regeneration ◽  
Plant Tissue ◽  
High Frequency ◽  
Sesamum Indicum ◽  
Direct Seeding ◽  
The Other ◽  
Seeding Method ◽  
Regeneration Response ◽  
High Sucrose

High frequency shoot regeneration was attempted in sesame (Sesamum indicum L. Pedaliaceae), using five genotypes/varieties viz. Tumkur and Gulbarga Locals (land races), W-II, E-8 and DS-1 (varieties). The hypocotyl-derived callus obtained through direct seeding method was placed on MS with five different treatments viz. pre-culture of callus on high sucrose (6 - 9%) for two weeks and transferring on to plain MS with 3% sucrose, MS with variable concentrations of TDZ alone, MS with constant TDZ and variable concentrations of BA and IAA, MS with variable concentrations of BA and IAA without TDZ, MS with ABA and AgNO3 and MS with BAP, NAA and AgNO3.  Highest frequency of cent per cent shoot regeneration was initiated in variety DS-1on MS containing NAA 2.5 mg/l, BAP 3.5 mg/l and 20 µM AgNO3 with 2.50 mean shoots/callus followed by 91.6% in variety W-II on MS containing 25 µM TDZ with 2.20 mean shoots/callus. The other treatments had poor shoot regeneration response. The results have been discussed in the light of sesame improvement through biotechnological ways.   Key words: Sesamum indicum, hypocotyl, high frequency,  shoot regeneration   D. O. I. 10.3329/ptcb.v21i1.9562   Plant Tissue Cult. & Biotech. 21(1): 45-52, 2011 (June)

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