A two-step protocol for shoot regeneration from hypocotyl explants of oilseed rape and its application for Agrobacterium-mediated transformation

2011 ◽  
Vol 55 (1) ◽  
pp. 21-26 ◽  
Author(s):  
G. -X. Tang ◽  
K. Knecht ◽  
X. -F. Yang ◽  
Y. -B. Qin ◽  
W. -J. Zhou ◽  
...  
Keyword(s):  
Oilseed Rape ◽  
Shoot Regeneration ◽  
Hypocotyl Explants ◽  
Agrobacterium Mediated Transformation

Shoot regeneration and Agrobacterium-mediated transformation of Fragaria vesca L.

1996 ◽  
Vol 15 (8) ◽  
pp. 642-646 ◽  
Author(s):  
Iman El Mansouri ◽  
José A. Mercado ◽  
Victoriano Valpuesta ◽  
José M. López-Aranda ◽  
Fernando Pliego-Alfaro ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Fragaria Vesca ◽  
Agrobacterium Mediated Transformation

scholarly journals The effect of carbon source on plant regeneration in tomato

2011 ◽  
Vol 32 (No. 1) ◽  
pp. 6-8 ◽  
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
L. Klčová
Keyword(s):  
Plant Regeneration ◽  
Carbon Source ◽  
Shoot Regeneration ◽  
Regeneration Capacity ◽  
Hypocotyl Explants ◽  
Cotyledon Explants ◽  
Tomato Cultivars ◽  
Number Of Shoots

 The effect of different tomato cultivars and different sugar types (sucrose, glucose and maltose) and concentrations (1.0, 2.0 and 3.0%) on shoot regeneration from aseptically grown hypocotyl and cotyledon explants were studied. Among sugar types, sucrose at a concentration of 3.0% induced the highest number of shoots from both types of explants. In hypocotyl explants, cv. Premium showed the best regeneration capacity (0.23 shoots per explant), and in cotyledon explants, cv. Hana produced the maximal number of shoots (0.43 or 0.37 for media with 2.0% or 3.0% sucrose, respectively).  

Shoot regeneration and Agrobacterium -mediated transformation of Fragaria vesca L.

1996 ◽  
Vol 15 (8) ◽  
pp. 642-646 ◽  
Author(s):  
Iman El Mansouri ◽  
Jos� A. Mercado ◽  
Victoriano Valpuesta ◽  
Jos� M. L�pez-Aranda ◽  
Fernando Pliego-Alfaro ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Fragaria Vesca ◽  
Agrobacterium Mediated Transformation

scholarly journals Agrobacterium-mediated transformation of Amaranthus cruentus L. epicotyls by the ARGOS-LIKE transgene

2020 ◽  
Author(s):  
R. M. Taipova ◽  
B. R. Kuluev
Keyword(s):  
Arabidopsis Thaliana ◽  
Shoot Regeneration ◽  
35S Promoter ◽  
Hygromycin B ◽  
Ms Medium ◽  
Amaranthus Cruentus ◽  
Agrobacterium Mediated Transformation ◽  
Murashige Skoog ◽  
Naphthylacetic Acid ◽  
Selection Of

The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.

Agrobacterium ‐Mediated Transformation of Oilseed Rape ( Brassica napus )

2017 ◽  
Vol 2 (4) ◽  
pp. 287-298 ◽  
Author(s):  
Ruth Bates ◽  
Melanie Craze ◽  
Emma J. Wallington
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Agrobacterium Mediated Transformation
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