Diagnostic immunohistochemistry of cutaneous metastatic breast carcinoma: A statistical analysis of the utility of gross cystic disease fluid protein-15 and estrogen receptor protein

Abstract Context.—Breast carcinoma often metastasizes to the gastrointestinal tract, especially the stomach, where it is frequently difficult to distinguish from a primary gastric carcinoma. Objective.—To evaluate the utility of immunohistochemical stains in differentiating primary gastric carcinomas from metastatic breast carcinomas. Design.—Mucosal biopsy specimens from 47 adenocarcinomas involving the gastrointestinal tract (28 primary gastric carcinomas and 19 metastatic breast carcinomas) and 16 control cases of primary breast carcinomas without metastasis were immunohistochemically stained for estrogen receptor protein (ER), progesterone receptor protein (PR), gross cystic disease fluid protein (GCDFP), human epidermal growth factor receptor 2 protein, cytokeratin (CK) 5/6, CK/7, CK/20, a panel of mucin glycoprotein antigens (MUC2, MUC3, MUC5AC, and MUC6), monoclonal antibody DAS-1, and caudal-type homeobox transcription factor CDX2 and compared between primary and metastatic adenocarcinomas. Results.—Highly significant proportions of metastatic breast carcinomas were positive for ER (72%), PR (33%), GCDFP (78%), and CK5/6 (61%) compared with primary gastric carcinomas (ER, 0%; PR, 0%; GCDFP, 0%; and CK5/6, 14%) (P < .001, P = .002, P < .001, and P = .004, respectively). Of these immunostains, ER, PR, and GCDFP were 100% specific. Primary breast tumors and their metastases showed a similar phenotypic profile. In contrast, primary gastric carcinomas showed significantly higher proportions of cases that stained with CK20 (50%), MUC2 (54%), MUC5AC (71%), MUC6 (39%), DAS-1 (43%), and CDX2 (67%) compared with metastatic breast carcinomas (CK20, 0%; MUC2, 24%; MUC5AC, 6%; MUC6, 0%; DAS-1, 0%; and CDX2, 0%) (P = .001, P = .01, P < .001, P = .02, P = .009, and P < .001, respectively). No significant differences were observed with regard to any of the other immunostains (human epidermal growth factor receptor 2 protein, CK7, and MUC3) between the patient groups. Conclusions.—Estrogen receptor protein, PR, GCDFP, CK5/6, CK20, MUC5AC, MUC6, DAS-1, and CDX2 are helpful in distinguishing primary gastric carcinomas from metastatic breast carcinomas. Of these, ER, PR, and GCDFP are highly specific for metastatic breast carcinomas, whereas CK20, DAS-1, MUC2, MUC5AC, MUC6, and CDX2 are highly specific for primary gastric carcinomas.

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Correlation of estrogen receptor protein and carcinoembryonic antigen with lymphnodal metastasis in breast carcinoma

Journal of Surgical Oncology ◽

10.1002/jso.2930370406 ◽

1988 ◽

Vol 37 (4) ◽

pp. 239-241 ◽

Cited By ~ 1

Author(s):

Anil K. Khemani ◽

Kusum Kapila ◽

K. R. Sundaram ◽

Kusum Verma

Keyword(s):

Estrogen Receptor ◽

Breast Carcinoma ◽

Carcinoembryonic Antigen ◽

Receptor Protein ◽

Estrogen Receptor Protein

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Correlation between estrogen receptor protein and carcinoembryonic antigen in normal and carcinomatous human breast tissue.

Clinical Chemistry ◽

10.1093/clinchem/22.8.1366 ◽

1976 ◽

Vol 22 (8) ◽

pp. 1366-1371 ◽

Cited By ~ 46

Author(s):

C J Menendez-Botet ◽

J S Nisselbaum ◽

M Fleisher ◽

P P Rosen ◽

A Fracchia ◽

...

Keyword(s):

Estrogen Receptor ◽

Breast Carcinoma ◽

Carcinoembryonic Antigen ◽

Metastatic Carcinoma ◽

Normal Breast ◽

Receptor Protein ◽

Human Breast ◽

Estrogen Receptor Protein ◽

Low Concentrations ◽

Tissue Specimens

Abstract We determined estrogen receptor protein and carcinoembryonic antigen in cytosols prepared from 189 human breast carcinoma tissues, 85 benign or normal breast biopsies, and 101 tissue specimens metastatic from breast carcinoma. Carcinoembryonic antigen was observed in 70% of the primary carcinomatous tissues, 15% of the benign or normal specimens, and 51% of the metastases. Ninety-six of the 189 primary carcinomatous specimens with increased concentrations of carcinoembryonic antigen were also positive for estrogen receptor protein, whereas 67 of the 72 benign or normal biopsies with low concentrations of carcinoembryonic antigen were also negative for estrogen receptor protein. All five fenign specimens with positive estrogen receptor protein and normal carcinoembryonic antigen concentrations were from fibroadenomas. The concordance between estrogen receptor protein and carcinoembryonic antigen in the primary carcinomatous tissue was 66%, in metastatic carcinoma 51%, and in benign and normal tissue 85%.

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Estrogen-receptor protein in intracranial meningiomas

Journal of Neurosurgery ◽

10.3171/jns.1979.50.4.0499 ◽

1979 ◽

Vol 50 (4) ◽

pp. 499-502 ◽

Cited By ~ 163

Author(s):

Mark S. Donnell ◽

Glenn A. Meyer ◽

William L. Donegan

Keyword(s):

Estrogen Receptor ◽

Breast Carcinoma ◽

Tumor Tissue ◽

Receptor Protein ◽

Estrogen Receptor Protein ◽

Content Type ◽

High Concentrations ◽

Very High ◽

Progressive Course ◽

Fine Print

✓ The increased frequency of meningiomas in women compared with men, and the rapidly progressive course of these tumors in pregnant patients suggest that hormones may be involved in this disease. Tumor tissue from six patients with meningiomas was analyzed for estrogen-receptor protein. Two patients had tumors with very high concentrations of this protein, approaching that found in hormonally sensitive breast carcinoma. The biochemical and possible clinical significance of these findings is discussed.

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