Correlation between estrogen receptor protein and carcinoembryonic antigen in normal and carcinomatous human breast tissue.

1976 ◽  
Vol 22 (8) ◽  
pp. 1366-1371 ◽  
Author(s):  
C J Menendez-Botet ◽  
J S Nisselbaum ◽  
M Fleisher ◽  
P P Rosen ◽  
A Fracchia ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Breast Carcinoma ◽  
Carcinoembryonic Antigen ◽  
Metastatic Carcinoma ◽  
Normal Breast ◽  
Receptor Protein ◽  
Human Breast ◽  
Estrogen Receptor Protein ◽  
Low Concentrations ◽  
Tissue Specimens

Abstract We determined estrogen receptor protein and carcinoembryonic antigen in cytosols prepared from 189 human breast carcinoma tissues, 85 benign or normal breast biopsies, and 101 tissue specimens metastatic from breast carcinoma. Carcinoembryonic antigen was observed in 70% of the primary carcinomatous tissues, 15% of the benign or normal specimens, and 51% of the metastases. Ninety-six of the 189 primary carcinomatous specimens with increased concentrations of carcinoembryonic antigen were also positive for estrogen receptor protein, whereas 67 of the 72 benign or normal biopsies with low concentrations of carcinoembryonic antigen were also negative for estrogen receptor protein. All five fenign specimens with positive estrogen receptor protein and normal carcinoembryonic antigen concentrations were from fibroadenomas. The concordance between estrogen receptor protein and carcinoembryonic antigen in the primary carcinomatous tissue was 66%, in metastatic carcinoma 51%, and in benign and normal tissue 85%.

Estrogen-receptor protein in intracranial meningiomas

1979 ◽  
Vol 50 (4) ◽  
pp. 499-502 ◽  
Author(s):  
Mark S. Donnell ◽  
Glenn A. Meyer ◽  
William L. Donegan
Keyword(s):  
Estrogen Receptor ◽  
Breast Carcinoma ◽  
Tumor Tissue ◽  
Receptor Protein ◽  
Estrogen Receptor Protein ◽  
Content Type ◽  
High Concentrations ◽  
Very High ◽  
Progressive Course ◽  
Fine Print

✓ The increased frequency of meningiomas in women compared with men, and the rapidly progressive course of these tumors in pregnant patients suggest that hormones may be involved in this disease. Tumor tissue from six patients with meningiomas was analyzed for estrogen-receptor protein. Two patients had tumors with very high concentrations of this protein, approaching that found in hormonally sensitive breast carcinoma. The biochemical and possible clinical significance of these findings is discussed.

scholarly journals Quantitation of the immunocytochemical assay for estrogen receptor protein (ER-ICA) in human breast cancer by television imaging.

1987 ◽  
Vol 35 (11) ◽  
pp. 1253-1259 ◽  
Author(s):  
R J Sklarew ◽  
L P Pertschuk
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Human Breast Cancer ◽  
Receptor Protein ◽  
Human Breast ◽  
Estrogen Receptor Protein ◽  
Er Positive ◽  
Immunocytochemical Assay ◽  
Nuclear Estrogen Receptor ◽  
Concentration Patterns

A Quantimet 720D Image Analysis System has been programmed for light microscopic evaluation of the nuclear estrogen receptor distribution in frozen sections of human breast cancer stained by the peroxidase-antiperoxidase method using monoclonal antibodies to estrogen receptor protein (ER). This method provides precise criteria for distinguishing ER-positive and -negative cells and a sensitive and reproducible means for densitometric quantification of the staining patterns. Although imaging sequence and graphic analysis are automated by computer programs, light pen interaction provides supervision of feature selection. Imaging of the immunocytochemical assay (ER-ICA) in 50 patients revealed marked heterogeneity of nuclear estrogen receptor concentration varying over a nearly 100 fold concentration range. Various ER concentration patterns were evident: (I) distributions with a single peak (CV = 5%) present at various concentration levels; (II) bimodal distributions, revealing co-existent ER-positive and ER-negative subpopulations; (III) multimodal distributions with a number of resolvable concentration peaks; and (IV) highly skewed distributions with or without discernible peaks, frequently extending over the entire concentration range. Statistical methods of de-convolution were applied to determine the frequency and ER concentration characteristics of component subpopulations in the mosaic cases and for resolving the proportion of ER-positive and -negative cells. An approach for evaluating nuclear ER content in conjunction with ER concentration patterns in individual patients revealed whether spread in the ER concentration distribution resulted from differences in nuclear ER content or from variability in nuclear volume distribution.

Estrogen receptor protein in visceral metastases from breast carcinoma

1981 ◽  
Vol 142 (2) ◽  
pp. 252-254 ◽  
Author(s):  
Frank E. Johnson ◽  
Paul Peter Rosen ◽  
Celia Menendez-Botet ◽  
Morton K. Schwartz ◽  
Roy Ashikari ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Breast Carcinoma ◽  
Receptor Protein ◽  
Estrogen Receptor Protein ◽  
Visceral Metastases

Activation of chromosomal vitellogenin genes in Xenopus oocytes by pure estrogen receptor and independent activation of albumin genes

1990 ◽  
Vol 10 (12) ◽  
pp. 6674-6682
Author(s):  
E A McKenzie ◽  
N A Cridland ◽  
J Knowland
Keyword(s):  
Estrogen Receptor ◽  
Xenopus Oocytes ◽  
Liver Cells ◽  
Receptor Protein ◽  
Estrogen Receptor Protein ◽  
Receptor Mrna ◽  
Estrogen Receptor Mrna ◽  
Vitellogenin Gene ◽  
Vitellogenin Genes

We generate pure estrogen receptor protein in Xenopus oocytes by injecting them with estrogen receptor mRNA synthesized in vitro. A chromosomal vitellogenin gene, which normally responds to estrogen only in liver cells, is activated. Primer extension shows that initiation is accurate, and ribonuclease mapping shows that the first exon is correctly spliced out of the initial transcript. Long transcripts are produced, one being equal in length to poly(A)- vitellogenin mRNA. Immunochemical estimates of receptor levels in the oocyte nuclei suggest that pure receptor, acting alone, cannot activate oocyte vitellogenin genes unless unusually large amounts are present. However, when a receptor-free extract from liver cells is also injected, the amount of receptor required is reduced. Such an extract, but not pure receptor, can also activate albumin genes in oocytes.

Estrogen receptor protein in breast cancer as a predictor of recurrence

Cancer ◽  
1981 ◽  
Vol 47 (10) ◽  
pp. 2364-2367 ◽  
Author(s):  
David W. Kinne ◽  
Roy Ashikari ◽  
Avital Butler ◽  
Celia Menendez-Botet ◽  
Paul Peter Rosen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Receptor Protein ◽  
Estrogen Receptor Protein

Estrogen receptor protein in adenomas of the large bowel

1982 ◽  
Vol 25 (4) ◽  
pp. 348-350 ◽  
Author(s):  
Michael V. Agrez ◽  
Robert J. Spencer
Keyword(s):  
Estrogen Receptor ◽  
Large Bowel ◽  
Receptor Protein ◽  
Estrogen Receptor Protein
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