Thymic hormonal activity on human peripheral blood lymphocytes, in vitro. IV. Proliferative response to allogeneic tumor cells in healthy adults and cancer patients

1983 ◽  
Vol 5 (6) ◽  
pp. 515-522 ◽  
Author(s):  
Jacob Shoham ◽  
Ilana Eshel
Keyword(s):  
Cancer Patients ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Proliferative Response ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Hormonal Activity ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

scholarly journals In vitro cytogenetic toxicity of bezafibrate in human peripheral blood lymphocytes

2017 ◽  
Vol 69 (4) ◽  
pp. 579-589 ◽  
Author(s):  
M. Topaktas ◽  
N. E. Kafkas ◽  
S. Sadighazadi ◽  
E. S. Istifli
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

Intracellular free magnesium in human lymphocytes and the response to lectins

1991 ◽  
Vol 80 (6) ◽  
pp. 539-547 ◽  
Author(s):  
L. L. Ng ◽  
J. E. Davies ◽  
M. C. Garrido
Keyword(s):  
Phaseolus Vulgaris ◽  
Peripheral Blood ◽  
Proliferative Response ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Fluorimetric Method ◽  
Human Peripheral Blood Lymphocytes ◽  
Free Magnesium

1. Intracellular free [Mg2+] was measured in human peripheral blood lymphocytes using a fluorimetric method based on the dye furaptra. It was necessary to correct for the extracellular leakage of the dye by using either 10 mmol/l EDTA or 0.05 mmol/l Mn2+. 2. As the proliferative response of lymphocytes to mitogenic lectins has been linked to a dependence on extracellular Mg2+, the intracellular [Mg2+] was studied in lymphocytes stimulated with various mitogenic and non-mitogenic lectins. 3. Only lymphocytes treated with phytohaemagglutinin-L, a leucoagglutinin from Phaseolus vulgaris that binds to tri- and tetra-antennary complex glycoproteins, showed a marked increase in intracellular [Mg2+]. This effect was partially inhibited by N-acetylgalactosamine. The stimulation by different lectins of the incorporation of [3H]-thymidine into lymphocytes was not correlated to the changes in intracellular [Mg2+]. 4. The proliferative response of lymphocytes to lectins is therefore not wholly dependent on a rise in intracellular [Mg2+].

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