Quantification of whole virus particles (146S) of foot-and-mouth disease virus in the presence of virus subunits (12S), using monoclonal antibodies in a sandwich ELISA

Vaccine ◽  
1995 ◽  
Vol 13 (12) ◽  
pp. 1064-1075 ◽  
Author(s):  
J Crowther
Vaccine ◽  
2011 ◽  
Vol 29 (52) ◽  
pp. 9655-9662 ◽  
Author(s):  
Miguel A. Martín-Acebes ◽  
Ángela Vázquez-Calvo ◽  
Mónica González-Magaldi ◽  
Francisco Sobrino

2012 ◽  
Vol 27 (5) ◽  
pp. 316-319 ◽  
Author(s):  
Tong Lin ◽  
Junjun Shao ◽  
Huiyun Chang ◽  
Shandian Gao ◽  
Guozheng Cong ◽  
...  

Author(s):  
Sangeeta Baro ◽  
Krishna Sharma ◽  
Biswajyoti Sharma ◽  
Shantanu Tamuly ◽  
P. Deka ◽  
...  

The molecular epidemiological study of foot-and-mouth disease virus (FMDV) has been carried out from different outbreaks in Assam the present study is based on the nucleotide sequencingof circulating FMDV serotype. The samples were subjected to sandwich ELISA, multiplex-PCR and molecular phylogeny to identify the type species. The phylogenetic analysis of virus sequence revealed similarity with theBangladesh isolates in the major branching pattern. The serotype ‘O’has found to be dominant and responsible for most of the recentoutbreaks.Thepersistence of serotype ‘O’ and cytokines expression of IL-1á, IL-1â, IFN-á, TNF-á in blood of recovered animals were done by Real time PCR. The findings indicated that IL-1á, IFN-á and TNF-á genes were up-regulated upto 3 months post infection but IL-1â found to be down regulated with progression of recovery. The present study thus supports that real-time PCR is a powerful technique for reliable detection of persistent FMDV in recovered animals.


1993 ◽  
Vol 6 (3) ◽  
pp. 219-228 ◽  
Author(s):  
ALBINO ALONSO ◽  
MARIA DA PENHA D. GOMES ◽  
ALBERTO KNUST RAMALHO ◽  
ROSSANA ALLENDE ◽  
HORACIO BARAHONA ◽  
...  

2009 ◽  
Vol 24 (6) ◽  
pp. 566-572
Author(s):  
Shuai Song ◽  
Tong Lin ◽  
Jun-jun Shao ◽  
Shan-dian Gao ◽  
Guo-zheng Cong ◽  
...  

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