Detection of epoxide hydrolase in rat hepatoma cell lines and primary rat liver cells by immunoblotting

1987 ◽  
Vol 37 (3) ◽  
pp. 285-291
Author(s):  
John M. Hunt ◽  
Chong-Jie Guo ◽  
Pratibha A. Desai
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Epoxide Hydrolase ◽  
Hepatoma Cell ◽  
Liver Cells ◽  
Hepatoma Cell Lines ◽  
Rat Liver Cells ◽  
Rat Hepatoma

Molecular cloning of cDNA for rat argininosuccinate lyase and its expression in rat hepatoma cell lines

1986 ◽  
Vol 6 (5) ◽  
pp. 1722-1728
Author(s):  
M A Lambert ◽  
L R Simard ◽  
P N Ray ◽  
R R McInnes
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Transcriptional Activity ◽  
Hepatoma Cell ◽  
Cdna Clones ◽  
Argininosuccinate Lyase ◽  
Hepatoma Cell Lines ◽  
Liver Cdna Library ◽  
Nuclear Processing ◽  
Rat Hepatoma

Using antibody and plaque hybridization screening, we isolated rat argininosuccinate lyase (AS lyase) cDNA clones from a liver cDNA library prepared in the phage expression vector lambda gt11. Five overlapping cDNAs covering 1.7 kilobases of the estimated 2.0-kilobase AS lyase mRNA were characterized and confirmed as AS lyase sequences by hybrid selection. We examined the differential expression of AS lyase in rat liver and four rat hepatoma cell lines (7800C1, H4, HTC, and MH1C1). These cells exhibited a 60-fold range of AS lyase enzyme activity, with a direct correlation between activity, amount of AS lyase immunoreactive protein, and quantity of specific AS lyase mRNA. These observations suggest that the differences in AS lyase expression between rat liver and the hepatoma cell lines result from variations in AS lyase transcriptional activity or alterations in nuclear processing of AS lyase RNA.

scholarly journals Molecular cloning of cDNA for rat argininosuccinate lyase and its expression in rat hepatoma cell lines.

1986 ◽  
Vol 6 (5) ◽  
pp. 1722-1728 ◽  
Author(s):  
M A Lambert ◽  
L R Simard ◽  
P N Ray ◽  
R R McInnes
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Transcriptional Activity ◽  
Hepatoma Cell ◽  
Cdna Clones ◽  
Argininosuccinate Lyase ◽  
Hepatoma Cell Lines ◽  
Liver Cdna Library ◽  
Nuclear Processing ◽  
Rat Hepatoma

Using antibody and plaque hybridization screening, we isolated rat argininosuccinate lyase (AS lyase) cDNA clones from a liver cDNA library prepared in the phage expression vector lambda gt11. Five overlapping cDNAs covering 1.7 kilobases of the estimated 2.0-kilobase AS lyase mRNA were characterized and confirmed as AS lyase sequences by hybrid selection. We examined the differential expression of AS lyase in rat liver and four rat hepatoma cell lines (7800C1, H4, HTC, and MH1C1). These cells exhibited a 60-fold range of AS lyase enzyme activity, with a direct correlation between activity, amount of AS lyase immunoreactive protein, and quantity of specific AS lyase mRNA. These observations suggest that the differences in AS lyase expression between rat liver and the hepatoma cell lines result from variations in AS lyase transcriptional activity or alterations in nuclear processing of AS lyase RNA.

Different Expression of Esterase Variants in Rat Hepatic and Hepatom a-Derived Cell Lines Detected by Electrophoresis

1995 ◽  
Vol 50 (9-10) ◽  
pp. 664-668 ◽  
Author(s):  
Adel S. Afify ◽  
Yoshimitsu Yamazaki ◽  
Yu-ichi Kageyama ◽  
Shiro Yusa ◽  
Yoshikatsu Ogawa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Hepatoma Cell ◽  
Polyacrylamide Gel Electrophoresis ◽  
Liver Homogenate ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Hepatoma Cell Lines ◽  
Normal Rat ◽  
Transformed Cell Lines

Abstract Esterases in nine rat hepatic and hepatoma-derived cell lines and normal rat liver homogenate were detected by polyacrylamide gel electrophoresis coupled with active staining with a-naphthyl acetate or butyrate as a substrate. The esterase band patterns of the non-cancerous and oncogene-transformed cell lines were alike, but different from those of hepatoma cell lines and normal rat liver homogenate. The former groups of cells might have completely lost the characteristics of rat liver parenchymal cells, or else they might have their origin at cells other than liver parenchyma. The esterase patterns of the hepatoma cell lines (e.g., McA-RH7777) and the normal rat liver highly resembled with each other, exemplifying the slight biochemical deviation of cancer from normal cells. However, two-dimensional electrophoretogram for the McA-RH7777 cell line showed a prominent esterase spot {p/ 6.0-Mr 110 kDa) that was lacking in the normal liver. This result indicates that there is invariably some change in esterase expression between the cancer cells and the normal liver cells

Trail and fasl induced apoptosis in hepatocytes, embryonal liver cells and hepatoma cell lines

2001 ◽  
Vol 34 ◽  
pp. 99
Author(s):  
M. Waltemathe ◽  
B. Mundt ◽  
F. Kuhnel ◽  
S. Bortlik ◽  
C. Trautwein ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Liver Cells ◽  
Hepatoma Cell Lines ◽  
Induced Apoptosis

Effects of hexamethylene bisacetamide onα-fetoprotein, albumin, and transferrin production by two rat hepatoma cell lines

In Vitro ◽  
1982 ◽  
Vol 18 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Everard H. Hughes ◽  
Herman A. J. Schut ◽  
Snorri S. Thorgeirsson
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Hexamethylene Bisacetamide ◽  
Hepatoma Cell Lines ◽  
Rat Hepatoma
Sign in / Sign up

Export Citation Format

Share Document