Mitogenic and antimitogenic effects of pituitary adenylate cyclase-activating polypeptide (PACAP) in adult rat chromaffin cell cultures

1995 ◽  
Vol 189 (3) ◽  
pp. 135-138 ◽  
Author(s):  
Arthur S. Tischler ◽  
Jocelyn C. Riseberg ◽  
Robyn Gray
Keyword(s):  
Adenylate Cyclase ◽  
Cell Cultures ◽  
Chromaffin Cell ◽  
Adult Rat ◽  
Pituitary Adenylate Cyclase

Potentiation of mitogenesis in adult rat chromaffin cell cultures by immunosuppressive agent FK506

2004 ◽  
Vol 356 (1) ◽  
pp. 5-8 ◽  
Author(s):  
J.F. Powers ◽  
J.M. Brachold ◽  
K. Schelling ◽  
A.S. Tischler
Keyword(s):  
Cell Cultures ◽  
Chromaffin Cell ◽  
Immunosuppressive Agent ◽  
Adult Rat

scholarly journals Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Reduces Oxidative and Mechanical Stress-Evoked Matrix Degradation in Chondrifying Cell Cultures

2019 ◽  
Vol 20 (1) ◽  
pp. 168 ◽  
Author(s):  
Eszter Szentléleky ◽  
Vince Szegeczki ◽  
Edina Karanyicz ◽  
Tibor Hajdú ◽  
Andrea Tamás ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Mechanical Stress ◽  
Cell Cultures ◽  
Cellular Stress ◽  
Cartilage Destruction ◽  
Cartilage Matrix ◽  
Neural Cells ◽  
Protein Levels ◽  
Pituitary Adenylate Cyclase ◽  
Expression And Activity

Pituitary adenylate cyclase activating polypeptide (PACAP) is an endogenous neuropeptide also secreted by non-neural cells, including chondrocytes. PACAP signaling is involved in the regulation of chondrogenesis, but little is known about its connection to matrix turnover during cartilage formation and under cellular stress in developing cartilage. We found that the expression and activity of hyaluronidases (Hyals), matrix metalloproteinases (MMP), and aggrecanase were permanent during the course of chondrogenesis in primary chicken micromass cell cultures, although protein levels changed daily, along with moderate and relatively constant enzymatic activity. Next, we investigated whether PACAP influences matrix destructing enzyme activity during oxidative and mechanical stress in chondrogenic cells. Exogenous PACAP lowered Hyals and aggrecanase expression and activity during cellular stress. Expression and activation of the majority of cartilage matrix specific MMPs such as MMP1, MMP7, MMP8, and MMP13, were also decreased by PACAP addition upon oxidative and mechanical stress, while the activity of MMP9 seemed not to be influenced by the neuropeptide. These results suggest that application of PACAP can help to preserve the integrity of the newly synthetized cartilage matrix via signaling mechanisms, which ultimately inhibit the activity of matrix destroying enzymes under cellular stress. It implies the prospect that application of PACAP can ameliorate articular cartilage destruction in joint diseases.

scholarly journals Pituitary Adenylate Cyclase-Activating Polypeptide Receptors Mediating Insulin Secretion in Rodent Pancreatic Islets Are Coupled to Adenylate Cyclase But Not to PLC

Endocrinology ◽  
2002 ◽  
Vol 143 (4) ◽  
pp. 1253-1259 ◽  
Author(s):  
Francoise Jamen ◽  
Raymond Puech ◽  
Joel Bockaert ◽  
Philippe Brabet ◽  
Gyslaine Bertrand
Keyword(s):  
Insulin Secretion ◽  
Adenylate Cyclase ◽  
Pancreatic Islets ◽  
Pituitary Adenylate Cyclase
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