Simultaneous analysis of individual carotenoids, retinol, retinyl esters, and tocopherols in serum by isocratic non-aqueous reversed-phase HPLC

1993 ◽  
Vol 46 (4) ◽  
pp. 419-424 ◽  
Author(s):  
Arun B. Barua ◽  
Harold C. Furr ◽  
Diane Janick-Buckner ◽  
James A. Olson
1989 ◽  
Vol 12 (7) ◽  
pp. 1261-1280 ◽  
Author(s):  
Hubert E. May ◽  
Sung I. Koo

2017 ◽  
Vol 84 (1) ◽  
pp. 127-134 ◽  
Author(s):  
Toshihiro Nagasaki ◽  
Tomoko Koito ◽  
Suguru Nemoto ◽  
Hideki Ushio ◽  
Koji Inoue

1994 ◽  
Vol 40 (3) ◽  
pp. 411-416 ◽  
Author(s):  
A L Sowell ◽  
D L Huff ◽  
P R Yeager ◽  
S P Caudill ◽  
E W Gunter

Abstract We describe the use of HPLC with multiwavelength detection to measure retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, beta-carotene, and the linoleate, oleate, palmitate, and stearate esters of retinol in a single 200-microL serum sample. The method is sensitive enough to detect individual retinyl esters in fasting serum from a nonhyperlipidemic population and requires only 12 min for each sample. Serum concentration ranges and means are reported for retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and the sum of the retinyl esters from serum analyses of 3480 participants from several different studies.


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