Phagocytosis of polymorphonuclear leukocytes by guinea pig peritoneal macrophages: effects of serum and temperature in vitro

1992 ◽  
Vol 105 (4) ◽  
pp. 211-218
Author(s):  
Chiyuki Yamamoto
Keyword(s):  
Guinea Pig ◽  
Polymorphonuclear Leukocytes ◽  
Peritoneal Macrophages

scholarly journals MACROPHAGE-DIGESTED ANTIGEN AS INDUCER OF DELAYED HYPERSENSITIVITY

1971 ◽  
Vol 133 (3) ◽  
pp. 494-505 ◽  
Author(s):  
Margot N. Pearson ◽  
Sidney Raffel
Keyword(s):  
Immune Responses ◽  
Guinea Pig ◽  
Peritoneal Macrophages ◽  
Delayed Hypersensitivity ◽  
Sheep Erythrocytes

Sheep erythrocytes ingested by guinea pig peritoneal macrophages in vitro, and permitted to undergo digestion for various periods, were found after some hours to lose the capacity to induce antibodies while gaining the ability to invoke delayed hypersensitivity. These observations may be related to the known predilection of small molecular immunogens to act as good inducers of delayed reactivity and poor stimulators of antibody. They may be related also to the activity of mycobacterial adjuvant as a vehicle for the induction of delayed hypersensitivity on the basis that this melange activates macrophages to phagocytose and enzymatically degrade macromolecular antigens rapidly. The thesis that small fragments of antigenic molecules may preferentially invoke hypersensitivity can be interpreted on the basis of current concepts of multicellular involvements in immune responses.

In vitro killing of tumor cells by soluble products of activated guinea pig peritoneal macrophages

1980 ◽  
Vol 49 (2) ◽  
pp. 379-383 ◽  
Author(s):  
Somesh D. Sharma ◽  
Willy F. Piessens ◽  
Gardner Middlebrook
Keyword(s):  
Guinea Pig ◽  
Tumor Cells ◽  
Peritoneal Macrophages

scholarly journals Infectivity of amastigotes of Trypanosoma cruzi

1986 ◽  
Vol 28 (4) ◽  
pp. 205-212 ◽  
Author(s):  
Tecia Ulisses de Carvalho ◽  
Wanderley de Souza
Keyword(s):  
Trypanosoma Cruzi ◽  
Guinea Pig ◽  
Cell Line ◽  
Peritoneal Macrophages ◽  
Mouse Peritoneal Macrophages ◽  
Pig Serum

The infectivity amastigotes of Trypanosoma cruzi, isolated from the supernatant of the J774G8 macrophage-like cell line infected with trypomastigotes to normal macrophages in vitro was tested. After a period of 1 h of T. cruzi-macrophage interaction about 2% of the mouse peritoneal macrophages had ingested amastigotes. In contrast 12% of the macrophages had ingested epimastigotes. Treatment of the amastigotes with trypsin did not interfere with their ingestion by macrophages. Once inside the macrophages the amastigotes divided and after some days transformed into trypomastigotes. When i.p. inoculated into mice the amastigotes were highly infective, inducing high levels of parasitaemia and tissue parasitism. As previously described for trypomastigotes, amastigotes were not lysed when incubated in the presence of fresh guinea-pig serum. Contrasting with what has been described for trypomastigotes, the resistance of amastigotes to complement-mediated lysis persisted after treatment with trypsin.

scholarly journals Inhibition of In Vitro Synthesis of the Second (C2) and Fourth (C4) Components of Complement in Guinea Pig Peritoneal Macrophages by a Soybean Oil Emulsion

1979 ◽  
Vol 13 (3) ◽  
pp. 188-193 ◽  
Author(s):  
Robert C Strunk ◽  
Kathleen Kunke ◽  
Ray B Nagle ◽  
Claire M Payne ◽  
H Robert Harrison
Keyword(s):  
Guinea Pig ◽  
Soybean Oil ◽  
Peritoneal Macrophages ◽  
In Vitro Synthesis ◽  
Oil Emulsion

scholarly journals The release of an endogenous pyrogen from guinea pig leukocytes in vitro: a new model for investigating the role of lymphocytes in fevers induced by antigen in hosts with delayed hypersensitivity.

1977 ◽  
Vol 145 (5) ◽  
pp. 1288-1298 ◽  
Author(s):  
P Chao ◽  
L Francis ◽  
E Atkins
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Polymorphonuclear Leukocytes ◽  
Delayed Hypersensitivity ◽  
Endogenous Pyrogen ◽  
Phagocytic Cells ◽  
Relationship Of ◽  
The Relationship

Guinea pig periotoneal exudate (PE) cells incubated overnight in vitro with heat-killed Staphylococci released an endogenous pyrogen (EP) that could be assayed by intravenous injection in rabbits. The febrile responses were linearly related to the dosage of EP over an eightfold range. PE cells derived from guinea pigs with delayed hypersensitivity (DH) to bovine gamma globulin (BGG), also released EP when incubated with antigen in vitro. This reaction was specific and did not occur withe PE cells from normal or complete Freund's adjuvant-sensitized guinea pigs. Studies indicated that monos and/or polymorphonuclear leukocytes rather than lymphocytes were the source of EP. However, when incubated with BGG and sufficient dosages of BGG-sensitized lymphocytes, normal PE cells released EP over a 42 h period. These results suggest that antigen stimulates specifically sensitized lymphocytes to release an agent (perhaps a lymphokine) that activates phagocytic cells to release EP. This model offers unique advantages for investigating in vitro the role of the lymphocyte in antigen-induced fever in DH as well as the relationship of this lymphocyte-induced activity to other known biologic activities mediated by antigen stimulated lymphocytes.

scholarly journals Fibrinogen/fibrin on the surface of macrophages: detection, distribution, binding requirements, and possible role in macrophage adherence phenomena.

1975 ◽  
Vol 142 (6) ◽  
pp. 1377-1390 ◽  
Author(s):  
R B Colvin ◽  
H F Dvorak
Keyword(s):  
Cell Surface ◽  
Guinea Pig ◽  
Significant Interaction ◽  
Mononuclear Cells ◽  
Peritoneal Macrophages ◽  
Unexpected Finding ◽  
Fluorescent Staining ◽  
Clotting System ◽  
Viable Cells

The peritoneal cavity of guinea pigs proved to be a rich source of mononuclear cells (34-52%) with fibrinogen or fibrin (Fib) on their surface. The Fib was readily detected on the surface of viable cells in suspension by fluorescence microscopy using antisera to guinea pig fibrinogen. The fluorescent staining occurred either in a speckled distribution, similar to that of cytophilic IgG, or in a distinctive net-like pattern that probably represented fibrin formation on the cell surface. The binding of Fib to the cell surface required calcium, but not magnesium, in the medium and could occur in vitro during incubation in heparinized plasma that contained fibrinogen concentrations comparable to that in normal peritoneal fluid (0.58 mg/ml). Cell surface Fib was more susceptible to plasmin and trypsin digestion than surface cytophilic IgG. By morphologic and physiologic criteria, cells exhibiting surface Fib were chiefly, if not exclusively, macrophages. Granulocytes, erythrocytes, and lymphocytes from lymph node and thymus had no sppreciable Fib. Cells with surface Fib were rarely observed among mononuclear cells prepared by Ficoll-Hypaque sedimentation of guinea pig and human blood (1.4 and 4.6%, respectively). Pulmonary alveolar macrophages, functionally distinct from peritoneal macrophages, lacked surface Fib (0.8%). Polymerization of Fib on the surface of macrophages might participate in certain cell interactions, such as the adherence of peritoneal macrophages during the antigen-induced macrophage disappearance reactions. The unexpected finding of Fib binding to the surfaces of peritoneal macrophages raises the possibility of a biologically significant interaction between these cells and the clotting system.

scholarly journals Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties

2004 ◽  
Vol 13 (4) ◽  
pp. 263-268 ◽  
Author(s):  
Ivo Lebrun ◽  
Valéria Cavallaro ◽  
Luiz Juliano ◽  
Maria A. Juliano ◽  
Maria C. C. de Sousa e Silva
Keyword(s):  
Guinea Pig ◽  
High Performance ◽  
Culture Media ◽  
Biological Activities ◽  
Direct Action ◽  
Toxin Production ◽  
Peritoneal Macrophages ◽  
Guinea Pig Ileum ◽  
Active Pool

CASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others.In this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. In the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay (guinea pig ileum, rat uterus), phagocytosisin vitroof opsonized sheep red blood cells, and hydrogen peroxide (H2O2) release from mouse peritoneal macrophages.Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2release from macrophages previously activated with bacille Calmette Guérin.Using mass spectra the primary structure of the main peptide from pool III was obtained – INKKI, which corresponds to β-casein fragment 26-30.The immunostimulating action is probably related to a direct action in macrophage cells.

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