Cloning and characterization of a novel sequence-specific DNA-binding protein recognizing the negative regulatory element (NRE) region of the HIV-1 long terminal repeat

Gene ◽  
1991 ◽  
Vol 101 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Ida Calvert ◽  
Ze-quo Peng ◽  
Hsiang-fu Kung ◽  
Raziuddin
Keyword(s):  
Dna Binding ◽  
Long Terminal Repeat ◽  
Binding Protein ◽  
Regulatory Element ◽  
Dna Binding Protein ◽  
Terminal Repeat ◽  
Negative Regulatory Element ◽  
Hiv 1

scholarly journals Identification and Characterization of anEntamoeba histolyticaUpstream Regulatory Element 3 Sequence-specific DNA-binding Protein Containing EF-hand Motifs

2001 ◽  
Vol 276 (15) ◽  
pp. 11838-11843 ◽  
Author(s):  
Carol A. Gilchrist ◽  
Chris F. Holm ◽  
Molly A. Hughes ◽  
Joanna M. Schaenman ◽  
Barbara J. Mann ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Regulatory Element ◽  
Dna Binding Protein ◽  
Predicted Amino Acid Sequence ◽  
Motif Analysis ◽  
Mobility Shift ◽  
Dna Motif ◽  
Ef Hand

Thehgl5 gene ofEntamoeba histolyticais negatively regulated through the upstream regulatory element 3 (URE3) DNA motif TATTCTATT. This motif is also present and significant in the function of theE. histolytica fdxgene promoter. A yeast one-hybrid screen was used to identify anE. histolyticacDNA encoding a protein (URE3-BP) that recognized this DNA motif. Analysis of the predicted amino acid sequence demonstrated the presence of two EF-hand motifs but identified no canonical DNA binding motifs. URE3-BP, expressed in bacteria, demonstrated Ca2+-dependent and sequence-specific recognition of the URE3 DNA sequence as assessed by electrophoretic mobility shift assays. Antibodies raised against URE3-BP blocked the formation of the URE3 DNA-protein complex by native nuclear extracts. The URE3-BP protein was present in theE. histolyticanucleus and cytoplasm with an apparent molecular mass of 22.6 kDa. Our results represent the first use of a yeast genetic screen to identify, on the basis of function, a DNA-binding protein of an early branching eukaryote. Since the URE3 DNA can modulate gene expression in both a positive and negative manner, this protein may have more than one mechanism of interaction with transcriptional machinery. Characterization of URE3-BP should provide insight into transcription regulation and virulence control in this parasite.

Avian retrovirus pp32 DNA binding protein. Preferential binding to the promoter region of long terminal repeat DNA

Biochemistry ◽  
1984 ◽  
Vol 23 (2) ◽  
pp. 350-359 ◽  
Author(s):  
Raymond J. Knaus ◽  
Paul J. Hippenmeyer ◽  
Tapan K. Misra ◽  
Duane P. Grandgenett ◽  
Uwe R. Muller ◽  
...  
Keyword(s):  
Dna Binding ◽  
Long Terminal Repeat ◽  
Promoter Region ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Terminal Repeat ◽  
Repeat Dna ◽  
Preferential Binding
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