negative regulatory element
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2011 ◽  
Vol 29 ◽  
pp. e380
Author(s):  
T. Konoshita ◽  
S. Mizuno ◽  
Y. Makino ◽  
S. Wakahara ◽  
K. Arakawa ◽  
...  


2010 ◽  
Vol 39 (3) ◽  
pp. 902-912 ◽  
Author(s):  
Karine Gendron ◽  
Gerardo Ferbeyre ◽  
Nikolaus Heveker ◽  
Léa Brakier-Gingras


2010 ◽  
Vol 285 (48) ◽  
pp. 37884-37894 ◽  
Author(s):  
Tadasuke Komori ◽  
Asako Doi ◽  
Hiroto Furuta ◽  
Hiroshi Wakao ◽  
Naoyuki Nakao ◽  
...  


2009 ◽  
Vol 41 (10) ◽  
pp. 873-882 ◽  
Author(s):  
Y. Wu ◽  
W. Zhang ◽  
Y. Yang ◽  
B. Yu ◽  
A. Huang


2007 ◽  
Vol 102 (4) ◽  
pp. 1043-1050 ◽  
Author(s):  
Tadashi Konoshita ◽  
Sébastien Fuchs ◽  
Yasukazu Makino ◽  
Shigeyuki Wakahara ◽  
Isamu Miyamori


2006 ◽  
Vol 281 (38) ◽  
pp. 27753-27764 ◽  
Author(s):  
Makoto Hiromura ◽  
Futoshi Suizu ◽  
Masumi Narita ◽  
Keiichi Kinowaki ◽  
Masayuki Noguchi


2006 ◽  
Vol 188 (2) ◽  
pp. 515-524 ◽  
Author(s):  
Kartik Viswanathan ◽  
Poorna Viswanathan ◽  
Lee Kroos

ABSTRACT C signaling plays a key role in coordinating cell movement and differentiation during the multicellular developmental process of Myxococcus xanthus. C signaling regulates expression of genes induced after about 6 h into development, when cells are forming mounds. One gene whose expression depends absolutely on C signaling was identified by insertion of a transposable element at site Ω4406 which generated a transcriptional fusion between lacZ and an upstream promoter. We have investigated regulation of the Ω4406 promoter. A 5′ deletion revealed a negative regulatory element located between bp −533 and −100 relative to the transcriptional start site. In the absence of this element, the promoter was still developmentally regulated but about fourfold more active. Also, the truncated promoter region retained normal dependence on two developmental regulators, FruA and DevS, but lost its dependence on the C-signaling protein CsgA. We infer that C signaling partially overcomes the negative effect of the upstream element on activity of the Ω4406 promoter. Deletion of downstream DNA between bp 50 and 140 caused a threefold loss in expression, suggesting that a positive regulatory element lies in this region. Additional positive and negative regulatory elements are present in the region from bp −69 to −49, based on the effects of multiple-base-pair mutations. Within this region, a 5-bp element and a C-box-like sequence resemble sequences found in other developmentally regulated M. xanthus promoter regions, but the effects of single-base-pair changes in these sequences suggest that each functions uniquely. We conclude that regulation of the Ω4406 promoter involves multiple positive and negative regulatory elements located upstream and downstream of the region typically bound by RNA polymerase.



2005 ◽  
Vol 336 (1) ◽  
pp. 142-149 ◽  
Author(s):  
Yu Lu ◽  
De-Qiao Sheng ◽  
Zhi-Cheng Mo ◽  
Hong-Fan Li ◽  
Ning-Hua Wu ◽  
...  


2005 ◽  
Vol 25 (15) ◽  
pp. 6772-6788 ◽  
Author(s):  
Karen Rothfels ◽  
Jason C. Tanny ◽  
Enikö Molnar ◽  
Helena Friesen ◽  
Cosimo Commisso ◽  
...  

ABSTRACT The divergently transcribed DIT1 and DIT2 genes of Saccharomyces cerevisiae, which belong to the mid-late class of sporulation-specific genes, are subject to Ssn6-Tup1-mediated repression in mitotic cells. The Ssn6-Tup1 complex, which is required for repression of diverse sets of coordinately regulated genes, is known to be recruited to target genes by promoter-specific DNA-binding proteins. In this study, we show that a 42-bp negative regulatory element (NRE) present in the DIT1-DIT2 intergenic region consists of two distinct subsites and that a multimer of each subsite supports efficient Ssn6-Tup1-dependent repression of a CYC1-lacZ reporter gene. By genetic screening procedures, we identified DFG16, YGR122w, VPS36, and the DNA-binding proteins Rim101 and Nrg1 as potential mediators of NRE-directed repression. We show that Nrg1 and Rim101 bind simultaneously to adjacent target sites within the NRE in vitro and act as corepressors in vivo. We have found that the ability of Rim101 to be proteolytically processed to its active form and mediate NRE-directed repression not only depends on the previously characterized RIM signaling pathway but also requires Dfg16, Ygr122w, and components of the ESCRT trafficking pathway. Interestingly, Rim101 was processed in bro1 and doa4 strains but was unable to mediate efficient repression.



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