Semiautomated multisample analysis of amniotic fluid lipids by high-performance thin-layer chromatography—reflectance spectrodensitometry

1993 ◽  
Vol 615 (1) ◽  
pp. 142-147 ◽  
Author(s):  
Juan G. Alvarez ◽  
Jack Ludmir
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Layer Chromatography

Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography.

1980 ◽  
Vol 26 (3) ◽  
pp. 403-405
Author(s):  
M D Kolins ◽  
E Epstein ◽  
W H Civin ◽  
S Weiner
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatographic System ◽  
Continuous Development ◽  
One Dimensional ◽  
Layer Chromatography

Abstract We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.

Radial "high-performance" thin-layer chromatography used to assess fetal lung maturity

1990 ◽  
Vol 36 (5) ◽  
pp. 728-731 ◽  
Author(s):  
J R Mackenzie ◽  
M Truesdale
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Fetal Lung ◽  
Additional Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Lung Maturity

Abstract A radial "high-performance" thin-layer chromatographic (HPTLC) method is described by which the percentages and ratios of phosphatidylinositol, sphingomyelin, lecithin, phosphatidylethanolamine, phosphatidylglycerol, and dimethyl phosphatidylethanolamine may be determined simultaneously. An additional method for radial HPTLC determination of saturated phosphatidylcholine is described. We report results of application of these methods to greater than 2000 specimens of amniotic fluid from both diabetic and nondiabetic cases.

Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography.

1980 ◽  
Vol 26 (3) ◽  
pp. 403-405 ◽  
Author(s):  
M D Kolins ◽  
E Epstein ◽  
W H Civin ◽  
S Weiner
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatographic System ◽  
Continuous Development ◽  
One Dimensional ◽  
Layer Chromatography

Abstract We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.

Sign in / Sign up

Export Citation Format

Share Document